Role of Local Immunoglobulin E Production in the Pathophysiology of Eosinophilic Chronic Rhinosinusitis
Project/Area Number |
15K20201
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Otorhinolaryngology
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Research Institution | Osaka University |
Principal Investigator |
Takeda Kazuya 大阪大学, 医学部附属病院, 医員 (90734054)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 好酸球性副鼻腔炎 / IgE / 鼻腔細菌 / Th2細胞 / クラススイッチ / 鼻茸 / IgE / アレルギー / モノクローナル抗体 / Th2 / 常在菌 / 体細胞超変異 / 鼻副鼻腔常在菌 |
Outline of Final Research Achievements |
We generated monoclonal antibodies from IgE+ plasmablasts of nasal polyps(NPs) of ECRS patients. In the reactivity tests, we found that ~20% of expressed antibodies reacted with nasal bacteria. In vitro T cell stimulation assay with identified antigens revealed that patients had more bacteria-reactive Th2 cells than healthy individuals. High-throughput sequencing analysis unraveled that local IgEs were originated from polyp-residing IgG+ and/or IgA+ B cells. These results suggest that protective mucosal immunity against nasal bacteria might convert into allergic inflammation through IgE class-switching in ECRS patients.
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Report
(4 results)
Research Products
(10 results)
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[Presentation] Identification of nasal resident bacteria as causative allergens in eosinophilic chronic rhinosinusitis2016
Author(s)
Kazuya Takeda, Shuhei Sakakibara, Daisuke Motooka, Shota Nakamura, Kazuo Yamashita, Daron Standley, Masaki Hayama, Takashi Shikina, Hidenori Inohara, Hitoshi Kikutani
Organizer
16th International Congress of Immunology
Place of Presentation
Melbourne, Australia
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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