Oncolytic activity of HF10 for head and neck squamous cell carcinomas

• Project/Area Number: 15K20219
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Nagoya City University
• Principal Investigator: Esaki Shinichi 名古屋市立大学, 大学院医学研究科, 助教 (20620983)
• Co-Investigator(Renkei-kenkyūsha): Goshima Fumi 名古屋大学, 大学院・医学系研究科・ウイルス学, 講師 (70201499)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腫瘍溶解ウイルス療法 / 単純ヘルペスウイルス / 頭頸部扁平上皮癌 / 抗腫瘍ウイルス療法 / 抗腫瘍ウイルス / 扁平上皮癌

Outline of Final Research Achievements

In this study, we investigated the therapeutic potential of HF10 against head and neck squamous cell carcinoma (HNSCC). Two human HNSCC cell lines, a mouse SCC cell line were prepared. HF10 replicated well, and killed them. HF10 injection decreased ear tumor in vivo and prolonged survival. Pathologically, HF10 infection induced necrosis, where infiltrating CD8 T cells were observed. Granulocytes and CD8 T cells increased in the spleen, and several antitumor cytokines increased in the supernatant of splenic cells after stimulation with the tumor cells. The mice that had survived from tumor rejected re-challenge of the tumor cells. These result suggested that HF10 killed the HNSCC cells and induced antitumoral immunity, and that HF10 can be a promising agent for the treatment of HNSCC patients.

Research Products

• [Journal Article] Facial nerve palsy after reactivation of herpes simplex virus type 1 in diabetic mice (2015)
  • Author(s): Esaki S, Yamano K, Katsumi S, Minakata T, Murakami S
  • Journal Title: Laringoscope Volume: 125 Issue: 4
  • DOI: 10.1002/lary.24994
  • Peer Reviewed
• [Journal Article] Targeting HIF-1apha in a new orthotopic model of glioblastoma recapitulating the hypoxic tumor microenvironment. (2015)
  • Author(s): Nigim F, Cavanaugh J, Patel AP, Curry WT Jr, Esaki S, Kasper EM, Chi AS, Louis DN, Martuza RL, Rabkin SD, Wakimoto H
  • Journal Title: J Neuropathol Exp Neurol. Volume: 74 Issue: 7 Pages: 710-722
  • DOI: 10.1097/nen.0000000000000210
  • Peer Reviewed
• [Presentation] Transient fasting enhances replication and therapeutic activity of oncolytic HSV in glioblastoma therapy (2015)
  • Author(s): Esaki S, Rabkin SD, Martuza RL, Wakimoto H
  • Organizer: 9th international conference on oncolytic virus therapeutics
  • Place of Presentation: Boston(MA)
  • Year and Date: 2015-06-14
  • Int'l Joint Research