Protective effects of lutein in tight junction disruption that promotes progression of age-related macular degeneration

Research Project

Project/Area Number	15K20283
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Ophthalmology
Research Institution	Keio University
Principal Investigator	Narimatsu Toshio 慶應義塾大学, 医学部(信濃町), 助教 (00570350)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	網膜 / 加齢黄斑変性 / 抗酸化酵素 / タイトジャンクション / ルテイン / 網膜色素上皮
Outline of Final Research Achievements	Photo-induced disruption of tight junction in the retinal pigment epithelium was recovered only slowly. However, lutein administration reduced tissue ROS levels and promoted the recovery. Lutein prolonged photo-induced mRNAs of antioxydative enzymes, SOD1 and SOD2, and SOD activity. Tissue mRNA levels of a photo-induced inflammatory cytokine, MCP-1, was suppressed by lutein. Similar effect was observed in ARPE19 cell line. Lutein promoted recovery of disrupted tight junction reducing oxidative stress most likely by both scavenging ROS and inducing antioxydative enzymes.