Development of adhesion inhibition method for Candida biofilm using competitive mechanism of adhesin-like polysaccharides

• Project/Area Number: 15K20461
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tsurumi University
• Principal Investigator: SATO Maki 鶴見大学, 歯学部, 非常勤講師 (10712094)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 義歯床用レジン / デンチャープラーク / バイオフィルム / Candida

Outline of Final Research Achievements

In this study, we examined the inhibitory effects of denture plate resin coatings on the adherence of Candida to the resin. Three adhesin-like polysaccharides (mannan, galactomannan, and β-glucan), which are structural components of the Candida cell wall, were used as coatings. A concentration-dependent inhibition of Candida adherence to the denture plate resin was observed in the groups coated with either 1 or 10 mg/ml mannan, but neither galactomannan nor β-glucan showed any inhibition effect. Similarly, chitosan, another structural component of the cell wall, which is known to have antimycotic properties, was applied to the denture plate resin, but no effect was observed over the period of this study.