| Project/Area Number |
15K20497
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kurume University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 骨再生 / DDM / 象牙質 / FIB/SEM / 象牙細管 / 脱灰象牙質 |
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| Outline of Final Research Achievements |
The osteocytes of new bone tissue surrounding the transplanted DDM had the network through the intermediary of each cellular processes and formed the bone tissue.
Interestingly, their cellular processes of osteocytes also was extended into the dental tubule and the bone tissue with canaliculi was formed and filled in it of the DDM surface was revealed. Additionally, the energy-dispersive X-ray spectroscopy approach revealed higher levels of Ca and P in the dentinal tubules than in the matrix. The finding suggests that the existence of dentinal tubules is an important advantage of using the DDM.
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| Academic Significance and Societal Importance of the Research Achievements |
移植した脱灰象牙質(demineralized dentin matrix 以下；DDM)が骨形成を促すことを報告しin vivo研究は過去に多くあるが, 象牙細管が骨形成にどのように寄与しているかというメカニズムはDDM移植治療においていまだ不明である. 今回我々は, DDMがDDM周囲に形成された新生骨とどのようにコンタクトしているかFIB/SEM(focused ion-beam slicing and scanning electron microscopy)を用いてその境界面を三次元的に調査した. 本研究は骨再生医療の一助になると考える.
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