A study for the development of minimally invasive diagnosis system in oral cancer with TGFbeta-signal regulatory molecules

• Project/Area Number: 15K20513
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: HAGIWARA Sumitaka 愛知県がんセンター(研究所), 共通機器室, 研究員 (40547551)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 頭頸部扁平上皮癌 / 血液腫瘍マーカー / 血清CD109 / 血清CD44 / TGFβシグナル / エクソソーム / 細胞外分泌 / 腫瘍マーカー / 細胞外分泌タンパク質 / CD44 / CD109 / エキソソーム / 扁平上皮癌 / ＴＧＦβシグナル / 前癌病変

Outline of Final Research Achievements

We investigate the concentration of serum CD109 (sCD109) and serum CD44-variant 6 (sCD44v6) with ELISA from 56 head and neck squamous cell carcinoma patients before and after surgery. Upregulation of sCD109 concentration was found in the patients with T3 or T4 tumor size, with positive lymph node, and with stage IV, in comparison to the patients with T1 or T2 tumor size, with negative lymph node, and with stage I~ III respectively. Multiple liner regression analysis about preoperative sCD109 indicated the status of lymph node metastasis as the significantly related clinical factor. No significant relationship was detected between sCD44v6 concentration and each clinical factor, except for pathological stage comparison in preoperative period.

Academic Significance and Societal Importance of the Research Achievements

本研究の結果、術前血清CD109値を測定することにより頸部リンパ節転移の有無を診断したり、治療前後での相対的変化を解析することにより予後不良症例を予測することなどが可能になると思われた。頭頸部扁平上皮癌患者において血清CD109値を解析する方法は、一臨床検査として悪性度や予後予測の客観的評価のための新たな診断ツールになり得ると期待される。

Research Products

• [Journal Article] CD109 is a component of exosome secreted from cultured cells (2016)
  • Author(s): Sakakura H, Mii S, Hagiwara S, Kato T, Yamamoto N, Hibi H, Takahashi M, Murakumo Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 469 Issue: 4 Pages: 816-822
  • DOI: 10.1016/j.bbrc.2015.12.063
  • Peer Reviewed / Open Access
• [Presentation] 口腔扁平上皮癌患者における血清CD109の定量解析と臨床的意義に関する検討 (2019)
  • Author(s): 萩原純孝，花井信広，鈴木秀典，西川大輔，小出悠介，別府慎太郎，日比英晴
  • Organizer: 第37回日本口腔腫瘍学会総会・学術大会
• [Remarks] TGFβシグナル関連分子の発現を応用した新規腫瘍マーカーの開発
  • URL: https://www.pref.aichi.jp/cancer-center/hosp/02shinryo/kakuka/toukei.html