Development of new predictive factors for malignant alteration of oral cancer by the functional analysis of the factors in regard to loss of anchorage dependence.

• Project/Area Number: 15K20534
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Yamaguchi University
• Principal Investigator: TAKENAWA Takanori 山口大学, 医学部, 特別医学研究員 (30711270)
• Research Collaborator: HARADA Koji 山口大学, 大学院医学系研究科, 助教 (60253217) ; FERDOUS Tarannum 山口大学, 大学院医学系研究科, 学術研究員 (70647932) ; KURAMITSU Yasuhiro 山口大学, 大学院医学系研究科, 准教授 (50281811)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Calreticulin / 口腔癌 / 足場依存性喪失 / プロテオミクス / calreticulin / 機能解析 / 癌幹細胞 / 口腔扁平上皮癌 / 悪性腫瘍 / 腫瘍生物学 / 腫瘍診断学 / プロテオーム / タンパク質修飾

Outline of Final Research Achievements

We used QR-32 cells in this study which is a regressive murine fibrosarcoma cell clone; and QRsP-11, a progressive malignant tumor cell clone originated from QR-32. CALR is a Ca2+-binding protein. This study was aimed to determine the usefulness of CALR as a prognostic factor in patients with oral cancers. Our findings suggest that elevated expression of CALR might play an important role in tumor progression in oral cancer, and could be considered as a useful prognostic factor in oral cancer patients. Next, we tried to investigate the functions of CALR in oral squamous cell carcinoma cell lines by using CALR-siRNA. We transfected CALR-siRNA into HSC2 and HSC3 cells. Down-regulation of CALR resulted in growth inhibition and lower cell migration on HSC2 and HSC3. These results suggest that down-regulation of CALR expressions might conductive to the reduction of OSCC malignancy.Our data suggested that CALR may be a new predictive factor for malignant alteration of oral cancer.

Research Products

• [Journal Article] Calreticulin is a novel independent prognostic factor for oral squamous cell carcinoma. (2017)
  • Author(s): Koji Harada, Takanori Takenawa, Tarannum Ferdous, Yasuhiro Kuramitsu, Yoshiya Ueyama
  • Journal Title: Oncology Letters Volume: 13 Issue: 6 Pages: 4857-4862
  • DOI: 10.3892/ol.2017.6062
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gimeracil enhances the antitumor effect of cisplatin against oral squamous cell carcinoma cells in vitro and in vivo (2016)
  • Author(s): Koji Harada, Tarannum Ferdous, Toyoko Harada, Takanori Takenawa, Yoshiya Ueyama
  • Journal Title: Oncology Letters Volume: in press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Expression of calreticulin is a novel independent prognostic factor for oral squamous cell carcinoma (2016)
  • Author(s): Takanori Takenawa, Koji Harada, Tarannum Ferdous, Yoshiya Ueyama
  • Organizer: ESMO 2016
  • Place of Presentation: The Bella Center, Copenhagen (Denmark)
  • Year and Date: 2016-10-07
  • Int'l Joint Research
• [Presentation] 重度肥満患者における気管切開施行経験 (2015)
  • Author(s): 河井 由衣、内田 堅一郎、加藤 芳明、竹縄 隆徳、真野 隆充、上山 吉哉
  • Organizer: 第 63 回 NPO 法人日本口腔科学会中国・四国地方部会
  • Place of Presentation: 岡山県 岡山市 岡山大学鹿田キャンパス Junko Fukutake Hall
  • Year and Date: 2015-09-25