Application to root resorption prevention and elucidation of periodontal tissue metabolic activity mechanism by drug discovery using amelogenin peptide and Platelet Rich Plasma

• Project/Area Number: 15K20593
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Yoshimi Yuki 広島大学, 病院(歯), 病院助教 (50707081)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アメロゲニン / 歯根吸収 / セメント芽細胞

Outline of Final Research Achievements

In this study, we focused on functional amelogenin peptides and PRP which is considered effective for wound healing. We clarified the influence of these on the metabolic regulation mechanism of periodontal tissues and studied as the ultimate goal of establishing the root absorption prevention method using these.It was suggested that the combination of functional amelogenin peptide and PRP is effective for cell activity of cementoblasts. Root absorption is a condition caused by excessive absorption of cementum. This study suggested the possibility of developing a novel root absorption prevention method using functional amelogenin peptide which improves the cell activity of cementoblasts.

Research Products

• [Journal Article] Effects of C-terminal amelogenin peptides on the metabolism of osteoblasts. (2017)
  • Author(s): Tetsuya Awada, Ryo Kunimatsu* , Yuki Yoshimi, Naoto Hirose, Tomomi Mitsuyoshi, Keisuke Sumi, Kotaro Tanimoto
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 482(2) Issue: 4 Pages: 1154-1159
  • DOI: 10.1016/j.bbrc.2016.12.003
  • Peer Reviewed / Open Access
• [Journal Article] Effects of C-Terminal Amelogenin Peptide on the Proliferation of Human Cementoblast Lineage Cells. (2016)
  • Author(s): Yuki Yoshimi, Ryo Kunimatsu, Naoto Hirose, Tetsuya Awada, Mutsumi Miyauchi, Takashi Takata, Li Wu, Li Zhu, Pamela Denbesten, Kazuo Tanne, Kotaro Tanimoto
  • Journal Title: Journal of periodontology Volume: 4 Issue: 7 Pages: 1-20
  • DOI: 10.1902/jop.2016.150507
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The C-terminus of amelogenin enhances osteogenic differentiaton of human cementoblast lineage cells (2016)
  • Author(s): Ryo Kunimatsu, Yuki Yoshimi, Naoto Hirose, Tetsuya Awada, Mutsumi Miyauchi, Takashi Takata, Li Wu, Zhu Li, Denbesten PK, Kotaro Tanimoto
  • Journal Title: Journal of periodontal research Volume: 4 Issue: 2 Pages: 1-7
  • DOI: 10.1111/jre.12384
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] ヒト培養セメント芽細胞の代謝に対するフラボノイド類化合物バイカリンの影響 (2016)
  • Author(s): 木村 綾、國松 亮、吉見 友希、柄 優至、粟田 哲也、郡司 秀美、中島 健吾、宮内 睦美、高田 隆、谷本 幸太郎
  • Organizer: 第75回日本矯正歯科学会大会
  • Place of Presentation: 徳島県徳島市
  • Year and Date: 2016-11-07
• [Presentation] アメロゲニンペプチドが由来の異なる間葉系幹細胞に及ぼす影響について (2016)
  • Author(s): 安藤 和代、鷲見 圭輔、國松 亮、吉見 友希、粟田 哲也、中島 健吾、阿部 崇晴、谷本 幸太郎
  • Organizer: 第75回日本矯正歯科学会大会
  • Place of Presentation: 徳島県徳島市
  • Year and Date: 2016-11-07
• [Presentation] Application of a new amelogenin-peptide to regeneration of cementum (2015)
  • Author(s): Yuki Yoshimi, Ryo Kunimatsu, Tetsuya Awada, Naoto Hirose, Tomomi Mitsuyoshi, Keisuke Sumi, Hidemi Gunji, Kengo Nakajima, Aya Kimura, Mutsumi Miyauchi, Takashi Takata, Kotaro Tanimoto
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2015-11-18
• [Presentation] Amelogenin isoforms effect the proliferation in human periodontal tissue cells (2015)
  • Author(s): Yuki Yoshimi, Ryo Kunimatsu, Naoto Hirose, Tetsuya Awada, Tomomi Mitsuyoshi, Hidemi Gunji, Takashi Takata, Kazuo Tanne, Kotaro Tanimoto
  • Organizer: 8th International orthodontic congress
  • Place of Presentation: EXCEL London, London（UK）
  • Year and Date: 2015-09-27
  • Int'l Joint Research