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Development of control system for periodontopathic biofilm using SspB peptide

Research Project

Project/Area Number 15K20610
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionNihon University

Principal Investigator

ITO Tatsuro  日本大学, 松戸歯学部, 講師 (60635126)

Research Collaborator SENPUKU Hidenobu  国立感染症研究所, 細菌第一部第六室, 室長 (20250186)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords小児歯科学 / バイオフィルム / SspBペプチド / 小児歯科
Outline of Final Research Achievements

To understand the three-way interaction among S. gordonii, P. gingivalis and salivary gp340 as a unit, we established a peptide binding assay using SspB (390-T400K-402). P. gingivalis 33277 showed the highest binding activity of the tested bacteria, whereas P. gingivalis W83, which was deficient in Mfa1 fimbriae, exhibited poor binding activity, as did S. gordonii. The binding of SspB (390-T400K-402) peptide in saliva- or gp340-treated P. gingivalis was significantly higher than that in non-treated cells. The SspB (390-T400K-402) peptide binding assay revealed that initial attachment of P. gingivalis to the substrata of S. gordonii may require gp340-mediated SspB-Mfa1 interactions. The assay is available to assess the relationships among SspB, Mfa1 and salivary gp340 as a unit. Taken together, a peptide binding assay using SspB (390-T400K-402) presented herein may provide important insights into the development of control system for periodontopathic biofilm.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Streptococcal Adhesin SspA/B Analogue Peptide Inhibits Adherence and Impacts Biofilm Formation of Streptococcus mutans.2017

    • Author(s)
      Ito T, Ichinosawa T, Shimizu T
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 4 Pages: e0175483-e0175483

    • DOI

      10.1371/journal.pone.0175483

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] <b>Molecular Interaction of the Analogous Peptide SspB (390-T400K-402) derived from </b><b><i>Streptococcus gordonii </i></b><b>Surface Protein Peptide with Periodontal Bacteria </b>2017

    • Author(s)
      Ichinosawa T, Ito T, Yonezawa H, Senpuku H, Shimizu T
    • Journal Title

      International Journal of Oral-Medical Sciences

      Volume: 15 Issue: 3-4 Pages: 160-167

    • DOI

      10.5466/ijoms.15.160

    • NAID

      130006894938

    • ISSN
      1347-9733, 2185-4254
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Streptococcal SspB Peptide Analog Inhibits Saliva-Promoted Adhesion and Biofilm Formation of Streptococcus mutans2016

    • Author(s)
      Tatsuro Ito, Takahiro Ichinosawa, Nana Ikematsu-Ito, Chihiro Watanabe, Takehiko Shimizu
    • Journal Title

      Open Journal of Stomatology

      Volume: 6 Issue: 03 Pages: 81-89

    • DOI

      10.4236/ojst.2016.63010

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] SspB Peptide Assay Reveals Saliva-Mediated Porphyromonas gingivalis Attachment2015

    • Author(s)
      Tatsuro Ito, Hidenobu Senpuku, Takahiro Ichinosawa, Nana Ikematsu-Ito, Nao Kimura, Takehiko Shimizu
    • Journal Title

      Open Journal of Stomatology

      Volume: 5 Issue: 11 Pages: 259-267

    • DOI

      10.4236/ojst.2015.511032

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] SspA/B ペプチドによる Streptococcus mutans のバイオフィルム抑制効果の検討2017

    • Author(s)
      伊藤龍朗, 市野澤隆宏, 清水武彦
    • Organizer
      第55回日本小児歯科学会大会
    • Place of Presentation
      西日本総合展示場新館・AIM展示場(福岡県北九州市)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Research-status Report
  • [Presentation] SspA/BペプチドによるStreptococcus mutansのバイオフィルム抑制効果の検討2017

    • Author(s)
      伊藤 龍朗、市野澤 隆宏、清水 武彦
    • Organizer
      第55回日本小児歯科学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 初期付着阻害による齲蝕原性バイオフィルム抑制効果の検討2017

    • Author(s)
      伊藤 龍朗, 市野澤 隆宏,清水 武彦
    • Organizer
      日大口腔科学会 第17回学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Streptococcal SspB peptide analog inhibits biofilm formation of Streptococcus mutans.2016

    • Author(s)
      Tatsuro Ito, Kazuhisa Okamoto, Masanobu Hara, Takahiro Ichinosawa, Takehiko Shimizu
    • Organizer
      10th Biennial Conference of PDAA 2016
    • Place of Presentation
      東京ドームホテル(東京都文京区)
    • Year and Date
      2016-05-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Streptococcal SspB peptide analog inhibits biofilm formation of Streptococcus mutans2016

    • Author(s)
      Tatsuro Ito, Kazuhisa Okamoto, Masanobu Hara, Takahiro Ichinosawa, Takehiko Shimizu
    • Organizer
      10th Biennial Conference of PDAA 2016
    • Place of Presentation
      東京ドームホテル(東京都文京区)
    • Year and Date
      2016-05-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新規バイオフィルム実験モデルの確立へ向けて2015

    • Author(s)
      伊藤 龍朗, 市野澤 隆宏, 清水 武彦
    • Organizer
      日本小児歯科学会関東地方会 第30回記念大会
    • Place of Presentation
      シェーンバッハ・サボー(東京都千代田区)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] SspBペプチドによるStreptococcus mutansのバイオフィルム制御法の確立2015

    • Author(s)
      市野澤 隆宏, 伊藤 龍朗, 清水 武彦
    • Organizer
      日大口腔科学会 第15回学術大会
    • Place of Presentation
      日本大学松戸歯学部(千葉県松戸市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] Treatment of eruption disturbance of permanent successor after trauma to primary tooth: 2 cases reports2015

    • Author(s)
      Nana Ikematsu-Ito, Tatsuro Ito, Kanako Sawamoto, Rina Shimada, Takehiko Shimizu
    • Organizer
      IAPD 2015 Conference
    • Place of Presentation
      Glasgow, United Kingdom
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Establishment of SspB peptide binding assay for Porphyromonas gingivalis colonization processes2015

    • Author(s)
      Tatsuro Ito, Takahiro Ichinosawa, Nana Ikematsu-Ito, Takehiko Shimizu
    • Organizer
      IAPD 2015 Conference
    • Place of Presentation
      Glasgow, United Kingdom
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] SspBペプチドによるStreptococcus mutans初期付着阻害効果の検討2015

    • Author(s)
      市野澤 隆宏, 倉田 香, 倉田 康弘, 伊藤 龍朗, 清水 武彦
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯周病リスク診断用ペプチド結合アッセイの確立2015

    • Author(s)
      伊藤 龍朗, 市野澤 隆宏, 荒井 延子, 伊藤(池松)奈々, 清水 武彦
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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