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Porphyromonas gingivalis is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm.

Research Project

Project/Area Number 15K20623
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontology
Research InstitutionKyushu University

Principal Investigator

Haraguchi Akira  九州大学, 大学病院, 助教 (00734998)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯周病細菌 / P. gingivalis / A. actinomycetemcomitans / 歯周病学 / 口腔内細菌 / Porphyromonas gingivalis
Outline of Final Research Achievements

P. gingivalis and A. actinomycetemcomitans is one of the most etiologically important microorganisms in periodontal disease. The aim of this study was investigated the protein of P. gingivalis supernatant about biofilm detachment of A. actinomycetemcomitans, and inhibition of aggregation of A. actinomycetemcomitans and clarified the factor of mechanism of detachment.
P. gingivalis culture supernatant which fractionated by ion exchange chromatography was not shown significant difference by a detachment of A. actinomycetemcomitans biofilm. In the two-dimensional electrophoresis of A. actinomycetemcomitans bacteria treated with the culture supernatant of P. gingivalis, decomposition of an unspecified number of proteins was observed even in the whole bacterial bodies and those extracted from membrane fractions. It was suggested that membrane protein of A. actinomycetemcomitans was dismantled nonselectively by proteases of P. gingivalis supernatant.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (1 results)

  • [Journal Article] Antibiotic effects against periodontal bacteria in organ cultured tissue.2017

    • Author(s)
      Takeshita M, Haraguchi A, Miura M, Hamachi T, Fukuda T, Sanui T, Takano A, Nishimura F.
    • Journal Title

      Clinical and Experimental Dental Research

      Volume: 3(1) Issue: 1 Pages: 5-12

    • DOI

      10.1002/cre2.48

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Innate immune-stimulatory activity of Porphyromonas gingivalis fimbriae is eliminated by phase separation using Triton X-114.2017

    • Author(s)
      Nozoe K, Sanui T, Takeshita M, Fukuda T, Haraguchi A, Aida Y, Nishimura F.
    • Journal Title

      Journal of Immunological Methods

      Volume: 441 Pages: 31-38

    • DOI

      10.1016/j.jim.2016.11.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Anti-CD14 antibody-treated neutrophils respond to LPS: Possible involvement of CD14 upregulated by anti-CD14 antibody binding.2017

    • Author(s)
      Sanui T, Takeshita M, Fukuda T, Haraguchi A, Aida Y, Nishimura F.
    • Journal Title

      Immunological Investigations

      Volume: 46(2) Issue: 2 Pages: 190-200

    • DOI

      10.1080/08820139.2016.1238925

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 紫外線照射が歯内疾患関連細菌および歯髄細胞に及ぼす影響2016

    • Author(s)
      原口 晃、吉田晋一郎、竹下正章、角 保徳、西村英紀、前田英史、和田尚久
    • Organizer
      日本歯科保存学会 2016年度秋季学術大会(第145回)
    • Place of Presentation
      キッセイ文化ホール
    • Year and Date
      2016-10-27
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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