A role of amelotin in apoptosis and epithelial-mesenchymal transition of gingival junctional epithelium

• Project/Area Number: 15K20630
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Nihon University
• Principal Investigator: NAKAYANA Yohei 日本大学, 松戸歯学部, 講師 (30434088)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯肉接合上皮 / アメロチン / アポトーシス / 慢性歯周炎 / TGFβ1 / Smad3 / 付着上皮

Outline of Final Research Achievements

Amelotin (AMTN) is an enamel protein that localized at gingival junctional epithelium (JE), however its function remain unclear. It's important for the understanding of biological response against periodontitis to evaluate homeostasis and pathology of JE. In this study, we examined the change of AMTN gene expression in gingival epithelium induced apoptosis, and observed AMTN localization at JE in Smad3 knockout mice. AMTN mRNA levels were temporally increased at TGFβ1-induced apoptosis of gingival epithelium. Smad3 was mediated in the transcriptional upregulation. Thus, AMTN might play a role in apoptosis of gingival epithelium.

Research Products

• [Journal Article] Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva. (2016)
  • Author(s): Nakayama Y, Kobayashi R, Matsui S, Matsumura H, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T, Yoshimura A, Shinomura T, Ganss B, Ogata Y.
  • Journal Title: Odontology Volume: - Issue: 3 Pages: 329-337
  • DOI: 10.1007/s10266-016-0277-y
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFb1 in mouse gingival epithelial cells (2016)
  • Author(s): Yohei Nakayama, Sari Matsui, Keisuke Noda, Mizuho Yamazaki, Yasunobu Iwai, Hiroyoshi Matsumura, Takashi Izawa, Eiji Tanaka, Bernhard Ganss and Yorimasa Ogata
  • Journal Title: Apoptosis Volume: 21 Issue: 10 Pages: 1057-70
  • DOI: 10.1007/s10495-016-1279-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enamel Hypomineralization and Structural Defects in Amelotin-deficient Mice. (2015)
  • Author(s): Nakayama Y, Holcroft J, Ganss B.
  • Journal Title: J Dent Res. Volume: Feb.24 Issue: 5 Pages: 1-9
  • DOI: 10.1177/0022034514566214
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 歯肉上皮細胞におけるTGFβ1によるアメロチン遺伝子発現の調節 (2016)
  • Author(s): 中山洋平，松井沙莉，能田佳祐，山崎瑞穂，岩井泰伸，松村浩禎，小方頼昌
  • Organizer: 日本歯科保存学会2016年度春季学術大会
  • Place of Presentation: 栃木県総合文化センター(栃木県宇都宮市)
  • Year and Date: 2016-06-09
• [Presentation] 歯肉上皮細胞におけるTGFβ1によるアメロチン遺伝子発現の調節 (2016)
  • Author(s): 中山洋平，松井沙莉，能田佳祐，山崎瑞穂，岩井泰伸，松村浩禎，小方頼昌
  • Organizer: 第144回春季日本歯科保存学会学術大会
  • Place of Presentation: 「栃木県総合文化センター(栃木県宇都宮市)」
  • Year and Date: 2016-06-09