Development of a microfluidic well plate for CTC detection
Project/Area Number |
15K20933
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
Medical systems
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Research Institution | The University of Tokyo |
Principal Investigator |
Kaneda Shohei 東京大学, 生産技術研究所, 助教 (10542467)
|
Research Collaborator |
FUJII Teruo
COLLARD Dominique
LAGADEC Chann
MEIGNAN Samuel
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | バイオ関連機器 / がん / マイクロ・ナノデバイス / マイクロ流体デバイス / 癌 |
Outline of Final Research Achievements |
In this project, a microfluidic well plate to separate circulating tumor cell (CTC) from peripheral blood were developed. The microfluidic well plate has 96 wells for CTC separation and each well connected to a single outlet via a microfluidic network. Different kinds of cell analysis against separated CTCs can be independently performed depending on each well. In evaluation tests, separation of PC-3 cells (prostate cancer cell line) spiked into peripheral blood obtained from healthy donor, and following viability assay using fluorescent stain (Calcein AM and Propidium Iodide) and immunofluorescence (FITC labeled anti-EpCAM antibody) had been successfully performed on the plate.
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Report
(3 results)
Research Products
(7 results)
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[Book] “Microfluidic Approach to Cell Handling and Measurement,” Sone, J. eds, Intelligent Nanosystems for Innovations in Energy, Environment, and Biotechnologies2016
Author(s)
Kawada, J., Kaneda, S., Kim, SH., Fujii, T.
Total Pages
21
Publisher
Springer-Verlag
Related Report
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