| Project/Area Number |
15K20933
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
Medical systems
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kaneda Shohei 東京大学, 生産技術研究所, 助教 (10542467)
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| Research Collaborator |
FUJII Teruo
COLLARD Dominique
LAGADEC Chann
MEIGNAN Samuel
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | バイオ関連機器 / がん / マイクロ・ナノデバイス / マイクロ流体デバイス / 癌 |
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| Outline of Final Research Achievements |
In this project, a microfluidic well plate to separate circulating tumor cell (CTC) from peripheral blood were developed. The microfluidic well plate has 96 wells for CTC separation and each well connected to a single outlet via a microfluidic network. Different kinds of cell analysis against separated CTCs can be independently performed depending on each well. In evaluation tests, separation of PC-3 cells (prostate cancer cell line) spiked into peripheral blood obtained from healthy donor, and following viability assay using fluorescent stain (Calcein AM and Propidium Iodide) and immunofluorescence (FITC labeled anti-EpCAM antibody) had been successfully performed on the plate.
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