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Analysis of ABA signal transduction system in algae

Research Project

Project/Area Number 15K20985
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Molecular biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Kobayashi Yuki  東京工業大学, 科学技術創成研究院, 助教 (80644616)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsアブシシン酸 / 植物ホルモン / 細胞周期 / 藻類 / 進化 / アブシジン酸 / テトラピロール
Outline of Final Research Achievements

ABA is a phytohormone, which acts the tolerance of abiotic stresses in land plants. In a recent study, we found that ABA is synthesized in primitive red alga Cyanidioschyzon merolae under salt stress condition and prevents the cell-cycle initiation. However, ABA signal transduction system in C. merolae is remain unclear. While land plants contain a specific ABA receptor protein, no counterpart has been found in algal species. Other ABA signal transduction components in the land plants, PP2C and SnRK2 proteins, exist on the C. merolae genome. In this study, we constructed over expression or antisense strain of CmPP2C and CmSnRK2. The CmPP2C antisense and CmSnRK2 over expression strains showed ABA response under non-ABA treatment condition. Covertly, the CmPP2C over expression and CmSnRK2 antisense strain did not show the ABA response under ABA treatment condition. These observations suggested that C. merolae has an ABA signal transduction system similar to that in land plants.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] 植物ホルモン「アブシシン酸」獲得のルーツ2017

    • Author(s)
      小林勇気、田中 寛
    • Journal Title

      化学と生物

      Volume: 55 Pages: 256-262

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Abscisic acid participates in the control of cell-cycle initiation through heme homeostasis in the unicellular red alga Cyanidioschyzon merolae.2016

    • Author(s)
      Yuki Kobayashi, Hiroyuki Ando, Mitsumasa Hanaoka, and Kan Tanaka
    • Journal Title

      Plant Cell Physiol.

      Volume: in press Issue: 5 Pages: 953-960

    • DOI

      10.1093/pcp/pcw054

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional regulation of tetrapyrrole biosynthetic genes explains abscisic acid-induced heme accumulation in the unicellular red alga Cyanidioschyzon merolae2016

    • Author(s)
      Yuki Kobayashi and Kan Tanaka
    • Journal Title

      Front. Plant Sci.

      Volume: -

    • DOI

      10.3389/fpls.2016.01300

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Extraction and Measurement of Abscisic Acid in a Unicellular Red Alga Cyanidioschyzon merolae2016

    • Author(s)
      Yuki Kobayashi and Kan Tanaka
    • Journal Title

      Bio-protocol

      Volume: 6 Issue: 23

    • DOI

      10.21769/bioprotoc.2033

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Construction of a <i>URA5.3</i> deletion strain of the unicellular red alga <i>Cyanidioschyzon merolae</i>: A backgroundless host strain for transformation experiments2015

    • Author(s)
      Keiko Taki, Toshiyuki Sone, Yuki Kobayashi, Satoru Watanabe, Sousuke Imamura, and Kan Tanaka
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 61 Issue: 5 Pages: 211-214

    • DOI

      10.2323/jgam.61.211

    • NAID

      130005110096

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 共生による細胞進化;別個のオシレーションは如何に共役したか?2017

    • Author(s)
      田中寛、小林勇気
    • Organizer
      真核細胞のオルガネラ研究最前線
    • Place of Presentation
      大阪大学 蛋白質研究所
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 紅藻Cyanidioschyzon merolaeにおけるABAシグナル伝達の解析2017

    • Author(s)
      田中寛、小林勇気
    • Organizer
      第58回植物生理学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of a URA marker recycling system for genome editing in unicelluar red alga Cyanidioschyzon merolae2017

    • Author(s)
      竹村時空、小林勇気、田中寛
    • Organizer
      第58回植物生理学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンにおける核遺伝子の光転写活性化機構の解析2017

    • Author(s)
      吉川瞳子、小林勇気、瀧景子、今村壮輔、田中寛
    • Organizer
      第11回 日本ゲノム微生物学会
    • Place of Presentation
      慶應義塾大学 湘南藤沢キャンパス
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] Functional analysis of ABA in Cyanidioschyzon merolae2016

    • Author(s)
      Yuki Kobayashi, Hiroyuki Ando, Mitsumasa Hanaoka and Kan Tanaka
    • Organizer
      第57回日本植物生理学年会
    • Place of Presentation
      盛岡 岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Functional analysis of nitrogen responsive small G protein CmRAB5 in a unicellular red alga Cyanidioschyzon merolae2016

    • Author(s)
      Tokiaki Takemura, Yuki Kobayashi, Keiko Taki, Sousuke Imamura, Kan Tanaka
    • Organizer
      第57回日本植物生理学年会
    • Place of Presentation
      盛岡 岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Abscisic acid response in Cyanidioschyzon merolae.2016

    • Author(s)
      Yuki Kobayashi、 Kan Tanaka
    • Organizer
      1st Cyanidioschyzon merolae Symposium
    • Place of Presentation
      東京大学
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 紅藻Cyanidioschyzon merolaeにおけるアブシジン酸の機能2015

    • Author(s)
      小林勇気、安藤洸幸、華岡光正、田中寛
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸 ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolae(シゾン)の遺伝子改変ホスト株の分離2015

    • Author(s)
      瀧景子、曽根俊之、小林勇気、渡辺智、今村壮輔、田中寛
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸 ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Cyanidioschyzon merolaeにおける細胞内小胞輸送タンパク質CmRab5の機能解析2015

    • Author(s)
      竹村時空、小林勇気、瀧景子、今村壮輔、田中寛
    • Organizer
      第14回微生物研究会
    • Place of Presentation
      川崎市 明治大学 生田キャンパス
    • Year and Date
      2015-10-31
    • Related Report
      2015 Research-status Report
  • [Remarks] 原始紅藻シゾンでアブシシン酸が機能―植物ホルモン獲得のルーツを解明―

    • URL

      http://www.titech.ac.jp/news/2016/034858.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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