| Project/Area Number |
15K21064
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細菌付着 / オートトランスポーター / 接着分子 / アドヘシン / HRV3Cプロテアーゼ / ＨＲＶ３Ｃプロテアーゼ / 三量体型オートトランスポーターアドへシン / 精製方法 |
|---|
| Outline of Final Research Achievements |
Identification of a functional domain on the highly adhesive fiber protein on a bacterium, AtaA, was attempted by protease digestion of the fiber on cell surface with specific protease. The recognition sites for HRV3C protease were introduced into the five FGG domains existing in the fiber (3CAtaA_FGG1~5). Production of these mutant fibers on bacterial cells, and appearance of the adhesive and auto-agglutinative characteristics of those cells were confirmed. The disappearance of these characteristics by treatment with HRV3C protease was then observed, and as a result, N-head domain existing at the tip of the fiber was suggested to be important for the function of AtaA fiber.
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