| Project/Area Number |
15K21147
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
Experimental pathology
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| Research Institution | Kobe University |
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Principal Investigator |
Ito Keisuke 神戸大学, 医学研究科, 助教 (10575468)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | RET / GDNF / Rab11 / Dynein / 細胞外基質 / Integrin / 変異型RET / 腸管神経系 / 細胞内輸送 / CRISPR/Cas9 / β1-integrin / 末梢神経系 |
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| Outline of Final Research Achievements |
Appropriate intracellular receptor trafficking underlie normal physiological activities of cells. RET, a Glial cell line neurotrophic factor receptor plays various roles in organogenesis, but its intracellular trafficking is largely unknown. In this project, I generated a knockin mouse line that express RET-GFP fusion proteins, and analyzed intracellular trafficking of RET in living cells. I found that RET exhibited polarized trafficking pattern and this pattern largely depends on types of extracellular matrices. I also found that Rab11 and Dynein pathways regulate RET trafficking. Finally, a mutant RET that can induces multiple human diseases showed aberrant localization. Therefore, it is suggested that appropriate RET trafficking is essential for normal physiological activities, and disruption of localization pattern of mutant RET leads to pathogenesis.
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