in vivo applications of next-generation DDS carrier for achieving less-invasive radiation therapy

• Project/Area Number: 15K21156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics; Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kobe University
• Principal Investigator: Nishimura Yuya 神戸大学, 科学技術イノベーション研究科, 特命助教 (40728218)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ドラッグデリバリーシステム / バイオナノカプセル / 抗癌剤ドキソルビシン / 放射線増感剤 / ナノ粒子 / 放射線治療 / Affibody / in vivo / 無機ナノ粒子

Outline of Final Research Achievements

We have developed bio-nanocapsule derived from hepatitis B virus as a carrier for drug delivery system and achieved some progress in vitro. Therefore, to apply this carrier in a clinical application, we demonstrated availability in vivo. As the result, delivery of anticancer drug (doxorubicin) showed antitumor effect in mouse xenograft models. Next, we measured time course of biodistribution of PAATiOx with radiosensitization efficacy and showed accumulation within the tumor. Then, in combination with X-irradiation, antitumor effect was observed. Therefore, we indicated that this carrier is available in vivo.

Research Products

• [Journal Article] Affibody-displaying bio-nanocapsules effective in EGFR, typical biomarker, expressed in various cancer cells (2017)
  • Author(s): Yuya Nishimura, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 27(2) Issue: 2 Pages: 336-341
  • DOI: 10.1016/j.bmcl.2016.11.038
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of titanium dioxide nanoparticles modified with polyacrylic acid and H2O2 for use as a novel Radiosensitizer, (2016)
  • Author(s): Morita K, Miyazaki S, Numaki C, Ikeno S, Sasaki R, Nishimura Y, Ogino C, Kondo A,
  • Journal Title: Free Radical Research, Volume: 50(12) Issue: 12 Pages: 1319-1328
  • DOI: 10.1080/10715762.2016.1241879
  • Peer Reviewed
• [Presentation] Less-invasive anticancer efficacy by a combination of bio-nanocapsule/liposome complex and radiation therapy (2016)
  • Author(s): Yuya Nishimura, Takahiro Suzuki, Kenta Morita, Masao Nakayama, Sachiko Inubushi, Chiya Numako, Chiaki Ogino, Ryohei Sasaki, Akihiko Kondo
  • Organizer: International Biotechnology Symposium 2016
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] HER2受容体発現細胞を標的としたDDSキャリアの抗腫瘍効果の評価 (2016)
  • Author(s): 西村勇哉、鈴木貴弘、森田健太、荻野千秋、近藤昭彦
  • Organizer: 生物工学若手研究者の集い 夏のセミナー2016
  • Place of Presentation: ホテルコンチネンタル府中（東京都府中市）
  • Year and Date: 2016-07-16
• [Presentation] Affibody提示バイオナノカプセル/リポソーム融合粒子を用いたin vivoでの抗腫瘍効果 (2016)
  • Author(s): 西村勇哉、鈴木貴弘、森田健太、荻野千秋、近藤昭彦
  • Organizer: 化学工学会 第81年会（2016）
  • Place of Presentation: 関西大学 千里山キャンパス
  • Year and Date: 2016-03-13
• [Presentation] Specific drug delivery for target cancer tumor using affibody-displaying bio-nanocapsule/liposome complex (2015)
  • Author(s): Yuya Nishimura, Jun Ishii, Chiaki Ogino, Akihiko Kondo
  • Organizer: PACIFICHEM 2015 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research