| Project/Area Number |
15K21394
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Tumor biology
Pathological medical chemistry
|
|---|
| Research Institution | Tokyo Medical and Dental University (2016)
Tokyo University of Pharmacy and Life Science (2015) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 内皮細胞 / 間葉系細胞 / 内皮間葉分化転換 / EndMT / 線維芽細胞増殖因子 / 腫瘍壊死因子 / 骨形成因子 / TGF-βシグナル / TNF-αシグナル / 間葉系細胞マーカー / 内皮間葉移行(EndMT) / 内皮細胞マーカー / ALK-1シグナル / BMP-9 |
|---|
| Outline of Final Research Achievements |
In this study, we established a line of mice in which endothelial and mesenchymal cells become labeled by red and green fluorescent proteins, respectively. Using these mice, endothelial cells which have undergone endothelial-to-mesenchymal transition (EndMT) can be specifically detectable. In addition, we developed a method of isolating endothelial cells from the mice and the isolated cells can be subject to in vitro analyses of EndMT. We successfully identified multiple types of signals regulating EndMT, such as ones mediated by tumor necrosis factor alpha, fibroblast growth factor and bone morphogenetic protein. Furthermore, we clarified a molecular mechanism by which their downstream pathways are regulated during the EndMT.
|
