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Development of cell discrimination method by using Raman spectroscopy and multivariate curve resolution

Research Project

Project/Area Number 15K21441
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Biological physics/Chemical physics/Soft matter physics
Research InstitutionWaseda University

Principal Investigator

ANDO MASAHIRO  早稲田大学, ナノ・ライフ創新研究機構, その他(招聘研究員) (50620803)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsラマン分光法 / 細胞 / 多変量解析 / 分子解析 / 非破壊分析 / 生細胞 / 分子レベル解析
Outline of Final Research Achievements

Raman microspectroscopy enables non-destructive and label-free molecular analysis of living cells. We have developed a new technique for discriminating cell types and different conditions through identifying molecular marker by using Raman spectroscopy. By applying multivariate spectral decomposition with multi-point measured Raman spectra that reflects the cellular internal structure, it has become possible to extract several molecular spectra that contributes to cell discrimination. It is characterized in that even the information on molecular structures can be considered by comparison with the authentic spectra of molecular markers.

Academic Significance and Societal Importance of the Research Achievements

生命現象の理解において、細胞を分子レベルで統合的に解析することは大変重要な課題である。蛍光顕微鏡に代表されるような、分子生物学や生化学に基づいた手法は、各種分子の高選択、高感度検出が可能であり、極めて高精度なレベルに達している。一方で、細胞内の構成分子を統合的に、非破壊・無染色で解析することは、幹細胞の分化制御や、医学臨床における細胞識別・状態解析など、幅広い応用研究において、今後必要性が増すものと考えられる。本研究では、ラマン分光法と多変量解析の手法を用いることで、分子科学的知見と共に、無染色で細胞を識別する新規技術を開発した。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2019 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results)

  • [Journal Article] Studying anti-oxidative properties of inclusion complexes of α-lipoic acid with γ-cyclodextrin in single living fission yeast by confocal Raman microspectroscopy2018

    • Author(s)
      Noothalapati Hemanth、Ikarashi Ryo、Iwasaki Keita、Nishida Tatsuro、Kaino Tomohiro、Yoshikiyo Keisuke、Terao Keiji、Nakata Daisuke、Ikuta Naoko、Ando Masahiro、Hamaguchi Hiro-o、Kawamukai Makoto、Yamamoto Tatsuyuki
    • Journal Title

      Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

      Volume: 197 Pages: 237-243

    • DOI

      10.1016/j.saa.2018.02.011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy2017

    • Author(s)
      Noothalapati Hemanth、Iwasaki Keita、Yoshimoto Chikako、Yoshikiyo Keisuke、Nishikawa Tomoe、Ando Masahiro、Hamaguchi Hiro-o、Yamamoto Tatsuyuki
    • Journal Title

      Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

      Volume: 187 Pages: 186-190

    • DOI

      10.1016/j.saa.2017.06.060

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Label-free Chemical Imaging of Fungal Spore Walls by Raman Microscopy and Multivariate Curve Resolution Analysis2016

    • Author(s)
      Hemanth Noothalapati, Takahiro Sasaki, Tomohiro Kaino, Makoto Kawamukai, Masahiro Ando, Hiro-o Hamaguchi, and Tatsuyuki Yamamoto
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 27789-27789

    • DOI

      10.1038/srep27789

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy2016

    • Author(s)
      Hemanth Noothalapati, Suguru Uemura, Naoki Ohshima, Yoshikazu Kinoshita, Masahiro, Ando, Hiro-o Hamaguchi, and Tatsuyuki Yamamoto
    • Journal Title

      Vibrational Spectroscopy

      Volume: 85 Pages: 7-10

    • DOI

      10.1016/j.vibspec.2016.03.016

    • NAID

      120005898770

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Simultaneous imaging of fat crystallinity and crystal polymorphic types by Raman microspectroscopy.2016

    • Author(s)
      Michiyo Motoyama, Masahiro Ando, Keisuke Sasaki, Ikuyo Nakajima, Koichi Chikuni, Katsuhiro Aikawa, Hiro-o Hamaguchi
    • Journal Title

      Food chemistry

      Volume: 196 Pages: 411-417

    • DOI

      10.1016/j.foodchem.2015.09.043

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Automatic and objective oral cancer diagnosis by Raman spectroscopic detection of keratin with multivariate curve resolution analysis2016

    • Author(s)
      P.-H. Chen, R. Shimada, S. Yabumoto, H. Okajima, M. Ando, C.-T. Chang, L.-T. Lee, Y.-K. Wong, A. Chiou, H. Hamaguchi
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 20097-20097

    • DOI

      10.1038/srep20097

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ラマン分光多変量スペクトル分解による生細胞の分子イメージング2019

    • Author(s)
      安藤正浩
    • Organizer
      レーザー学会学術講演会 第38回年次大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Label-free molecular distribution imaging of single-cells by Raman hyperspectral analysis2019

    • Author(s)
      Masahiro Ando
    • Organizer
      12th International workshop on approaches to single cell analysis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生細胞ラマン分光測定から非標識で 多数の分子分布画像を抽出2018

    • Author(s)
      安藤正浩
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 多変量ラマンスペクトル分解による脂質・タンパク質解析2017

    • Author(s)
      安藤正浩、浜口宏夫
    • Organizer
      医用分光学研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Lipid Composition Analysis of Cells and Tissues by Raman spectroscopy coupled with Multivariate Curve Resolution Analysis2016

    • Author(s)
      Masahiro Ando, Hiro-o Hamaguchi
    • Organizer
      The Fourth Taiwan International Symposium on Raman Spectroscopy
    • Place of Presentation
      台北
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Quantitative Raman Spectroscopy of Complex Systems by Hypothetical Addition Multivariate Analysis with Numerical Differentiation2015

    • Author(s)
      Masahiro Ando, Soshi Yabumoto, Hiro-o Hamaguchi
    • Organizer
      11th Australian Conference on Vibrational Spectroscopy/5th Asian Spectroscopy Conference
    • Place of Presentation
      Sydney
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新しいケモトリクス法「仮想添加多変量微分スペクトル解析」による複雑系のラマンスペクトルの定量解析2015

    • Author(s)
      安藤正浩, 浜口宏夫
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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