Development of cell discrimination method by using Raman spectroscopy and multivariate curve resolution

• Project/Area Number: 15K21441
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry; Biological physics/Chemical physics/Soft matter physics
• Research Institution: Waseda University
• Principal Investigator: ANDO MASAHIRO 早稲田大学, ナノ・ライフ創新研究機構, その他(招聘研究員) (50620803)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ラマン分光法 / 細胞 / 多変量解析 / 分子解析 / 非破壊分析 / 生細胞 / 分子レベル解析

Outline of Final Research Achievements

Raman microspectroscopy enables non-destructive and label-free molecular analysis of living cells. We have developed a new technique for discriminating cell types and different conditions through identifying molecular marker by using Raman spectroscopy. By applying multivariate spectral decomposition with multi-point measured Raman spectra that reflects the cellular internal structure, it has become possible to extract several molecular spectra that contributes to cell discrimination. It is characterized in that even the information on molecular structures can be considered by comparison with the authentic spectra of molecular markers.

Academic Significance and Societal Importance of the Research Achievements

生命現象の理解において、細胞を分子レベルで統合的に解析することは大変重要な課題である。蛍光顕微鏡に代表されるような、分子生物学や生化学に基づいた手法は、各種分子の高選択、高感度検出が可能であり、極めて高精度なレベルに達している。一方で、細胞内の構成分子を統合的に、非破壊・無染色で解析することは、幹細胞の分化制御や、医学臨床における細胞識別・状態解析など、幅広い応用研究において、今後必要性が増すものと考えられる。本研究では、ラマン分光法と多変量解析の手法を用いることで、分子科学的知見と共に、無染色で細胞を識別する新規技術を開発した。

Research Products

• [Journal Article] Studying anti-oxidative properties of inclusion complexes of α-lipoic acid with γ-cyclodextrin in single living fission yeast by confocal Raman microspectroscopy (2018)
  • Author(s): Noothalapati Hemanth、Ikarashi Ryo、Iwasaki Keita、Nishida Tatsuro、Kaino Tomohiro、Yoshikiyo Keisuke、Terao Keiji、Nakata Daisuke、Ikuta Naoko、Ando Masahiro、Hamaguchi Hiro-o、Kawamukai Makoto、Yamamoto Tatsuyuki
  • Journal Title: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Volume: 197 Pages: 237-243
  • DOI: 10.1016/j.saa.2018.02.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy (2017)
  • Author(s): Noothalapati Hemanth、Iwasaki Keita、Yoshimoto Chikako、Yoshikiyo Keisuke、Nishikawa Tomoe、Ando Masahiro、Hamaguchi Hiro-o、Yamamoto Tatsuyuki
  • Journal Title: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Volume: 187 Pages: 186-190
  • DOI: 10.1016/j.saa.2017.06.060
  • Peer Reviewed
• [Journal Article] Label-free Chemical Imaging of Fungal Spore Walls by Raman Microscopy and Multivariate Curve Resolution Analysis (2016)
  • Author(s): Hemanth Noothalapati, Takahiro Sasaki, Tomohiro Kaino, Makoto Kawamukai, Masahiro Ando, Hiro-o Hamaguchi, and Tatsuyuki Yamamoto
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 27789-27789
  • DOI: 10.1038/srep27789
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy (2016)
  • Author(s): Hemanth Noothalapati, Suguru Uemura, Naoki Ohshima, Yoshikazu Kinoshita, Masahiro, Ando, Hiro-o Hamaguchi, and Tatsuyuki Yamamoto
  • Journal Title: Vibrational Spectroscopy Volume: 85 Pages: 7-10
  • DOI: 10.1016/j.vibspec.2016.03.016
  • NAID: 120005898770
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Simultaneous imaging of fat crystallinity and crystal polymorphic types by Raman microspectroscopy. (2016)
  • Author(s): Michiyo Motoyama, Masahiro Ando, Keisuke Sasaki, Ikuyo Nakajima, Koichi Chikuni, Katsuhiro Aikawa, Hiro-o Hamaguchi
  • Journal Title: Food chemistry Volume: 196 Pages: 411-417
  • DOI: 10.1016/j.foodchem.2015.09.043
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Automatic and objective oral cancer diagnosis by Raman spectroscopic detection of keratin with multivariate curve resolution analysis (2016)
  • Author(s): P.-H. Chen, R. Shimada, S. Yabumoto, H. Okajima, M. Ando, C.-T. Chang, L.-T. Lee, Y.-K. Wong, A. Chiou, H. Hamaguchi
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 20097-20097
  • DOI: 10.1038/srep20097
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ラマン分光多変量スペクトル分解による生細胞の分子イメージング (2019)
  • Author(s): 安藤正浩
  • Organizer: レーザー学会学術講演会 第38回年次大会
  • Invited
• [Presentation] Label-free molecular distribution imaging of single-cells by Raman hyperspectral analysis (2019)
  • Author(s): Masahiro Ando
  • Organizer: 12th International workshop on approaches to single cell analysis
  • Int'l Joint Research
• [Presentation] 生細胞ラマン分光測定から非標識で 多数の分子分布画像を抽出 (2018)
  • Author(s): 安藤正浩
  • Organizer: 日本分析化学会第67年会
  • Invited
• [Presentation] 多変量ラマンスペクトル分解による脂質・タンパク質解析 (2017)
  • Author(s): 安藤正浩、浜口宏夫
  • Organizer: 医用分光学研究会
  • Invited
• [Presentation] Lipid Composition Analysis of Cells and Tissues by Raman spectroscopy coupled with Multivariate Curve Resolution Analysis (2016)
  • Author(s): Masahiro Ando, Hiro-o Hamaguchi
  • Organizer: The Fourth Taiwan International Symposium on Raman Spectroscopy
  • Place of Presentation: 台北
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Quantitative Raman Spectroscopy of Complex Systems by Hypothetical Addition Multivariate Analysis with Numerical Differentiation (2015)
  • Author(s): Masahiro Ando, Soshi Yabumoto, Hiro-o Hamaguchi
  • Organizer: 11th Australian Conference on Vibrational Spectroscopy/5th Asian Spectroscopy Conference
  • Place of Presentation: Sydney
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] 新しいケモトリクス法「仮想添加多変量微分スペクトル解析」による複雑系のラマンスペクトルの定量解析 (2015)
  • Author(s): 安藤正浩, 浜口宏夫
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京工業大学
  • Year and Date: 2015-09-16