| Project/Area Number |
15K21449
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic bioproduction science
Environmental dynamic analysis
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| Research Institution | Soka University |
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Principal Investigator |
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| Research Collaborator |
SANO Tomoharu 国立環境研究所, 環境計測研究センター, 主任研究員 (10178808)
TOMIOKA Noriko 国立環境研究所, 地域環境研究センター, 主任研究員 (40168399)
SHIMODE Shinji 横浜国立大学, 環境情報研究院, 准教授 (70397090)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | バクテリア生産 / 安定同位体 / LC-MS / DNA合成 / デオキシリボヌクレオシド / DNA / デオキシアデノシン / デオキシグアノシン / チミジン / LC/MS/MS |
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| Outline of Final Research Achievements |
Incorporation characteristics of four deoxyribonucleosides (thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine) composing DNA in natural bacterial communities were examined to evaluate appropriate deoxyribonucleosides for measuring bacterial production from the viewpoint of DNA synthesis rate. The highest incorporation rate among the four deoxyribonucleosides was deoxyguanosine. The present study suggests that measurement of bacterial production by using deoxyguanosine possibly reflects larger numbers of bacterial species productions.
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