Examination of the relationship between EGFR mutant allele frequency and efficacy of EGFR inhibitor of tissue samples and blood samples in patient with lung cancer

• Project/Area Number: 15K21688
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics; Tumor therapeutics
• Research Institution: Shizuoka Cancer Center Research Institute
• Principal Investigator: Akira Ono 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (50441027)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肺癌 / リキッドバイオプシー / allele frequency / 末梢血循環腫瘍DNA / 末梢血中循環腫瘍DNA / 体細胞遺伝子変異 / クローン / サブクローン / 腫瘍内不均一性 / 腫瘍診断学 / 分子標的治療 / デジタルPCR / EGFR遺伝子変異

Outline of Final Research Achievements

Ultrasensitive EGFR gene mutation measuring system was constructed using tissue samples and blood samples in non-small cell lung cancer patients.This result is significant in realizing quantification of EGFR gene mutation with non-invasive methoed. With development future genome medicine, we considered important in evaluating choice of treatment policy and efficacy of treatment based on the evaluation of allele frequency in EGFR gene mutation and other somatic gene mutataion.

Academic Significance and Societal Importance of the Research Achievements

侵襲性の低い血液検体での遺伝子検査方法の確立が、今後のがんゲノム医療の発展に寄与し得ると考える。

Research Products

• [Journal Article] Plasme epidermal growth factor recepter mutation testing with a chip-based digital PCR system in patients with advanced non-small cell lung cancer (2017)
  • Author(s): Kasahara N, Kenmotsu H, Serizawa M, et al.
  • Journal Title: Lung Cancer Volume: 106 Pages: 138-144
  • DOI: 10.1016/j.lungcan.2017.02.001
  • Peer Reviewed / Open Access
• [Journal Article] Plasma epidermal growth factor receptor mutation testing with achip-based digital PCR system in patients with advanced non-smallcell lung cancer (2017)
  • Author(s): Kasahara N, Kenmotsu H, Serizawa M, Umehara R, Ono A, Hisamatsu Y, Wakuda K, Omori S, Nakashima K, Taira T, Naito T, Murakami H, Koh Y, Mori K, Endo M, Nakajima T, Yamada M, Kusuhara M, Takahashi T.
  • Journal Title: Lung Cancer Volume: 106 Pages: 138-144
  • DOI: 10.1016/j.lungcan.2017.02.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Plasma epidermal growth factor recepter mutation (EGFR) testing in advanced non-small cell lung cancer patients harboring EGFR mutations by chip-based digital PCR system (2016)
  • Author(s): Norimitsu Kasahara, Hirotsugu Kenmotsu, Masakuni Serizawa, Rina Umehara, Akira Ono, Kazushige Wakuda, Shota Omori, Kazuhisa Nakashima, Tetsuhiko Taira, Tateaki Naito, Haruyasu Murakami, Noriaki Sunaga, Yasuhiro Koh, Keita Mori, Masahiro Endo, Takashi Nakajima, Masanobu Yamada, Masatoshi Kusuhara, Toshiaki Takahashi
  • Organizer: American Association for Cancer Research Annual Meeting 2016
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 2016-04-16 – 2016-04-20
  • Int'l Joint Research