Diversity and functions of chytrids in lakes and oceans: Develoment of detection methods and quantifying material flows(Fostering Joint International Research)

• Project/Area Number: 15KK0026
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: Yokohama National University (2018); Toho University (2015-2017)
• Principal Investigator: KAGAMI Maiko 横浜国立大学, 大学院環境情報研究院, 教授 (20449250)
• Research Collaborator: GROSSART Hans-Peter Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB-Berlin), Professor ; VAN DONK Ellen Netherlands Institute of Ecology (NIOO-KNAW), Professor
• Project Period (FY): 2015 – 2018
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: 物質循環 / 湖沼 / 環境変動 / 生物多様性 / 菌類 / 微生物 / 遺伝子解析 / 操作実験 / ツボカビ

Outline of Final Research Achievements

We aim to reveal the hidden diversity of chytrids and functions in lakes and oceans under the collaboration with Germany (IGB-Berlin) and the Netherlands (NIOO-KNAW). Combining molecular techniques (High throughput sequencing and Single cell PCR) with isolation or microscopic observations are powerful tools, by which we indeed discovered novel chytrid linages infecting phytoplankton. Chytrids play important roles in material cycling, as a excellent foods for zooplankton. In addition, chytrids affect the bacteria community through modifying algal quality.

Academic Significance and Societal Importance of the Research Achievements

ツボカビはカエルツボカビ症の蔓延や有害・有用藻類への寄生が発見されたことで、近年その重要性への認識が世界的にも高まっている。しかし、方法的な制約から実際に研究を進めているのは国内外問わず限られていた。今回、ドイツとオランダとの共同研究を行ったことで、ヨーロッパ諸国やアメリカとの共同研究へと発展させることでき、ツボカビの国際的なデータベースの整備が進んだ。このデータベースがツボカビ感染症の制御や発生予測の現場においても活用され、水質や水産資源管理の上でも役立つ可能性が高い。また、温暖化や富栄養化に伴う湖沼・海洋生態系の変遷など、水圏生態系の保全にも役立つものである。

Research Products

• [Int'l Joint Research] Netherlands Institute of Ecology (NIOO)(オランダ) (2017)
  • Year and Date: 2017-07-10
• [Int'l Joint Research] Leibniz-Institute of Freshwater Ecology and Inland Fisheries(ドイツ) (2016)
  • Year and Date: 2016-04-01
• [Journal Article] Fungi in aquatic ecosystems (2019)
  • Author(s): Grossart Hans-Peter、Van den Wyngaert Silke、Kagami Maiko、Wurzbacher Christian、Cunliffe Michael、Rojas-Jimenez Keilor
  • Journal Title: Nature Reviews Microbiology Volume: ー Issue: 6 Pages: 339-354
  • DOI: 10.1038/s41579-019-0175-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Taming chlorophylls allowed eukaryotes to prosper on the oxygenated Earth. (2019)
  • Author(s): Kashiyama Y, Yokoyama A, Shiratori T, Bachy C, Gutierrez-Rodriguez A, Not F, Hess S, Wang M, Chen M, Gong Y, Seto K, Kagami M, Hamamoto Y, Honda D, Umetani T, Shihongi A, Kayama M, Matsuda T, Taira J, Yabuki A, Tsuchiya M, Hirakawa Y, Kawaguchi A, Nomura M, Nakamura A, Namba N, Matsumoto M, Tanaka T, Yoshino T et al.
  • Journal Title: The ISME journal Volume: - Issue: 8 Pages: 1-12
  • DOI: 10.1038/s41396-019-0377-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fungi in the Marine Environment: Open Questions and Unsolved Problems (2019)
  • Author(s): Amend Anthony et al.
  • Journal Title: mBio Volume: 10 Issue: 2
  • DOI: 10.1128/mbio.01189-18
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Introducing ribosomal tandem repeat barcoding for fungi (2018)
  • Author(s): Wurzbacher Christian、Larsson Ellen、Bengtsson‐Palme Johan、Van den Wyngaert Silke、Svantesson Sten、Kristiansson Erik、Kagami Maiko、Nilsson R. Henrik
  • Journal Title: Molecular Ecology Resources Volume: 19 Issue: 1 Pages: 118-127
  • DOI: 10.1111/1755-0998.12944
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Influence of parasitic chytrids on the quantity and quality of algal dissolved organic matter (AOM) (2018)
  • Author(s): Senga Yukiko、Yabe Shiori、Nakamura Takaki、Kagami Maiko
  • Journal Title: Water Research Volume: 145 Pages: 346-353
  • DOI: 10.1016/j.watres.2018.08.037
  • NAID: 120006540109
  • Peer Reviewed
• [Journal Article] Diversity and Hidden Host Specificity of Chytrids Infecting Colonial Volvocacean Algae (2018)
  • Author(s): Van den Wyngaert Silke、Rojas-Jimenez Keilor、Seto Kensuke、Kagami Maiko、Grossart Hans-Peter
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 65 Issue: 6 Pages: 870-881
  • DOI: 10.1111/jeu.12632
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Spatial heterogeneity in induced defense of Brachionus calyciflorus within a single lake caused by the bed of floating-leaved macrophyte Trapa species (2018)
  • Author(s): Yurie Otake; Maiko Kagami, Takeo Kuriyama, Takehito Yoshida
  • Journal Title: Limnology Volume: ー
  • Peer Reviewed
• [Journal Article] Determining spatio-temporal variation in Lake Biwa fungal communities using high-throughput sequencing (2018)
  • Author(s): Peixue Song; Shoko Tanabe; Rong Yi; Maiko Kagami; Syuhei Ban
  • Journal Title: Limnology Volume: 19 Pages: 241-251
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phytoplankton species abundance in Lake Inba (Japan) from 1986 to 2016. (2017)
  • Author(s): Akemi Iwayama, Hisako Ogura, Yukio Hirama, Chun-Wei Chang, Chih-hao Hsieh, Maiko Kagami
  • Journal Title: Ecological Research Volume: 32 Issue: 6 Pages: 783-783
  • DOI: 10.1007/s11284-017-1482-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Vertical distributions of Pu and radiocesium isotopes in sediments from Lake Inba after the Fukushima Daiichi Nuclear Power Plant accident: source identification and accumulation. (2017)
  • Author(s): L. Cao, N. Ishii, J. Zheng, M. Kagami, S. M. Pan, K. Tagami, S. Uchida
  • Journal Title: Applied Geochemistry Volume: 78 Pages: 287-294
  • DOI: 10.1016/j.apgeochem.2017.01.012
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A new parasitic chytrid, Staurastromyces oculus (Rhizophydiales, Staurastromycetaceae fam. nov.), infecting the freshwater desmid Staurastrum sp. (2017)
  • Author(s): Silke Van den Wyngaert, Kensuke Seto, Keilor Rojas-Jimenez, Maiko Kagami, Hans-Peter Grossart
  • Journal Title: Protists Volume: 印刷中 Issue: 4 Pages: 392-407
  • DOI: 10.1016/j.protis.2017.05.001
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Integrating chytrid fungal parasites into plankton ecology. Research gaps and needs. (2017)
  • Author(s): Frenken T, Alacid E, Berger SA, Bourne EC, Gerphagnon M, Grossart HP, Gsell AS, Ibelings BW, Kagami M, Kupper FC, Letcher PM, Loyau A, Miki T, Nejstgaard JC, Rasconi S, Rene A, Rohrlack T, Rojas‐Jimenez K, Schmeller DS, Scholz B, Seto K, (略）Agha R
  • Journal Title: Environmental Microbiology Volume: 19 Issue: 10 Pages: 3802-3822
  • DOI: 10.1111/1462-2920.13827
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phylogenetic Position of Parasitic Chytrids on Diatoms: Characterization of a Novel Clade in Chytridiomycota (2017)
  • Author(s): Kensuke Seto, Maiko Kagami, Yousuke Degawa
  • Journal Title: The journal of Eukaryotic Microbiology Volume: 64 Issue: 3 Pages: 383-393
  • DOI: 10.1111/jeu.12373
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Geographical distrubutions of rhytismataceaous fungi on Camellia japonica leaf litter in Japan. (2017)
  • Author(s): Kimiyo Matsukura, Dai Hirose, Maiko Kagami, Takashi Osono, Yuichi Yamaoka
  • Journal Title: Fungal Ecology Volume: 26 Pages: 37-44
  • DOI: 10.1016/j.funeco.2016.11.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Carbon and nutrients of indigestible pollen are transferred to zooplankton by chytrid fungi. (2017)
  • Author(s): Maiko Kagami, Yasumichi Motoki, Helene Masclaux, Alexandre Bec
  • Journal Title: Freshwater Biology Volume: 62 Issue: 5 Pages: 954-964
  • DOI: 10.1111/fwb.12916
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of Daphnid Grazing on Microscopic Zoosporic Fungi using Comparative CT qPCR. (2016)
  • Author(s): Michelle Maier, Kimiko Uchii, Tawnya Peterson, and Maiko Kagami
  • Journal Title: Applied and Environmental Microbiology Volume: 82 Issue: 13 Pages: 3868-3874
  • DOI: 10.1128/aem.00087-16
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Discovery of dark matter fungi in aquatic ecosystems demands a reappraisal of the phylogeny and ecology of zoosporic fungi. (2016)
  • Author(s): Hans-Peter Grossart, Christian Wurzbacher, Timothy James, Maiko Kagami
  • Journal Title: Fungal Ecology Volume: 19 Pages: 28-38
  • DOI: 10.1016/j.funeco.2015.06.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Dark matter fungi: 様々な時空間スケールで水生菌類の機能的多様性をとらえる (2019)
  • Author(s): 鏡味麻衣子
  • Organizer: 日本生態学会第66回全国大会
• [Presentation] Dark matter fungi: 水生菌類の多様性と機能 (2018)
  • Author(s): 鏡味麻衣子
  • Organizer: 北海道大学低温科学研究所研究集会「水環境の保全と再生に向けた環境微生物学・水環境学の最前線」
  • Invited
• [Presentation] Mycoloopの先へ、これから挑戦したいこと (2018)
  • Author(s): 鏡味麻衣子
  • Organizer: 日本陸水学会第83回大会
• [Presentation] Epidemic dynamics of chytrids in aquatic food webs (2018)
  • Author(s): Maiko Kagami
  • Organizer: 3rd plankton chytridiomycosis workshop
  • Invited
• [Presentation] Single spore analysis reveals diverse host-parasite relationships between phytoplankton and fungi (2018)
  • Author(s): Maiko Kagami, Silke van den Wyngaert, Kensuke Seto, Christian Wurzbacher, Hans-Peter Grossart
  • Organizer: 日本微生物生態学会第32回大会
• [Presentation] Diversity and functions of parasitic fungi in aquatic ecosystems (2018)
  • Author(s): Maiko Kagami
  • Organizer: Marine Fungi Workshop
  • Invited
• [Presentation] 感染症と生態系機能：生物多様性および食物網を考慮に入れたプランクトン感染症動態 (2018)
  • Author(s): 鏡味麻衣子、瀬戸健介、三木健
  • Organizer: 日本生態学会第65回全国大会
  • Invited
• [Presentation] 珪藻寄生性ツボカビの多様性と生態学的機能ー分類学的研究から動態解析へー (2018)
  • Author(s): 瀬戸健介, 出川洋介, 鏡味麻衣子
  • Organizer: 日本生態学会第65回全国大会
• [Presentation] 珪藻寄生性菌類がバクテリア群集構造およびDOM組成に与える影響 (2017)
  • Author(s): 鏡味麻衣子，千賀有希子，Jason Woodhouse, Christian Wurzbacher, Hans-Peter Grossart
  • Organizer: 日本陸水学会第８２回大会
• [Presentation] 印旛沼におけるMicrocystis aeruginosaの遺伝的多様性と分布パターン (2017)
  • Author(s): 岡崎一眞, 田辺雄彦, 鏡味麻衣子
  • Organizer: 日本陸水学会第８２回大会
• [Presentation] Aulacoseira属珪藻寄生性ツボカビの系統的位置および宿主特異性 (2017)
  • Author(s): 瀬戸健介，鏡味麻衣子, 出川洋介
  • Organizer: 日本陸水学会第８２回大会
• [Presentation] Seasonal variation of fungal community, especially zoosporic fungi, in Lake Biwa based on LSU region of rDNA (2017)
  • Author(s): Peixue Song, Rong Yi, Shoko Tababe, Syuhei Ban, Maiko Kagami
  • Organizer: 日本陸水学会第８２回大会
• [Presentation] Parasitic fungi modulate algal-bacterial interactions and DOM composition (2017)
  • Author(s): Maiko Kagami, Yukiko Senga, Jason Woodhouse, Christian Wurzbacher, Hans-Peter Grossart
  • Organizer: 15th Symposium on Aquatic Microbial Ecology
  • Int'l Joint Research
• [Presentation] Phylogenetic, morphological and host range diversity of three parasitic chytrids, infecting colonial volvocacean algae (2017)
  • Author(s): Silke Van den Wyngaert, Keilor Rojas-Jimenez, Maiko Kagami, Hans-Peter Grossart
  • Organizer: 15th International Congress of Prositology
  • Int'l Joint Research
• [Presentation] Generalist v.s. Specialist chytrids: Trade-off between host specificity and ecological traits (2016)
  • Author(s): Maiko Kagami, Kensuke Seto, Christian Wurzbacher, Takaki Nakamura, Daiki Nozaki, Silke van den Wyngaert, Keilor Rojas, Hans-Peter Grossart
  • Organizer: 2nd Plankton Chytridiomycosis Workshop
  • Place of Presentation: Skagastrond、Iceland
  • Year and Date: 2016-09-15
  • Int'l Joint Research / Invited
• [Presentation] Taxonomic study of parasitic chytrids on algae -Taxonomy of genus Zygorhizidium (2016)
  • Author(s): Kensuke Seto, Maiko Kagami, Yousuke Degawa.
  • Organizer: 2nd Plankton Chytridiomycosis Workshop
  • Place of Presentation: Skagastrond、Iceland
  • Year and Date: 2016-09-15
  • Int'l Joint Research / Invited
• [Presentation] Chytrids in aquatic ecosystems: ubiquitous distribution in aquatic ecosystems? (2016)
  • Author(s): Grossart, H.P., Van den Wyngaert, S., Rojas-Jimenez, K., Bourne, C.L., Wurzbacher, C., Kagami, M
  • Organizer: 2nd Plankton Chytridiomycosis Workshop
  • Place of Presentation: Skagastrond、Iceland
  • Year and Date: 2016-09-15
  • Int'l Joint Research / Invited
• [Presentation] A tale of three chytrids and three ways of infecting one(?) host (2016)
  • Author(s): Van den Wyngaert, S., Rojaz-Jimenez, K., Grossart, H.P.
  • Organizer: 2nd Plankton Chytridiomycosis Workshop
  • Place of Presentation: Skagastrond、Iceland
  • Year and Date: 2016-09-15
  • Int'l Joint Research / Invited
• [Presentation] Single spore analysis reveals diversity and host specificity of parasitic fungi infecting phytoplankton (2016)
  • Author(s): Maiko Kagami, Daiki Nozaki, Kensuke Seto, Takaki Nakamura, Christian Wurzbacher
  • Organizer: International Society of Limnology 2016
  • Place of Presentation: Torino, Italy
  • Year and Date: 2016-07-31
  • Int'l Joint Research
• [Presentation] 珪藻寄生性ツボカビの分類学的研究：環境配列のみで認識された新規クレードの実態解明 (2016)
  • Author(s): 瀬戸健介、鏡味麻衣子、出川洋介
  • Organizer: 第28回日本菌学会関東支部年次大会
  • Place of Presentation: 玉川大学（東京都町田市）
  • Year and Date: 2016-04-23
• [Book] ときめく微生物図鑑 (2016)
  • Author(s): 鏡味 麻衣子(監修), 塩野 正道(写真),塩野 暁子(写真
  • Publisher: 山と渓谷社
• [Remarks] 横浜国立大学水域生態学研究室
  • URL: https://makagami.wixsite.com/mycoloop
• [Remarks] 横浜国立大学環境情報研究院水域生態学研究室
  • URL: https://makagami.wixsite.com/mycoloop
• [Remarks] 東邦大学理学部生命圏環境科学科湖沼生態学研究室
  • URL: http://www.lab.toho-u.ac.jp/sci/env/kagami/index.html