| Project/Area Number |
15KK0308
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Research Collaborator |
Lotz Martin スクリプス研究所, 分子医学部門, 教授
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| Project Period (FY) |
2016 – 2017
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
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| Keywords | 変形性関節症 / エクソソーム / マイクロRNA / 軟骨細胞 / microRNA / 軟骨 |
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| Outline of Final Research Achievements |
This study has focused on novel communication factor exosomes including microRNA (miRNA) in osteoarthritis (OA). We classified OA grade and performed miRNA expression profiling in human chondrocytes and exosomes using normal- and OA-derived chondrocytes. Several miRNAs were highly expressed in normal chondrocytes and chondrocytes-derived exosomes. These miRNAs were decreased in OA chondrocytes and OA chondrocytes-derived exosomes. Thus, we newly generated cartilage-specific miRNA transgenic mice and have examined the effect of prevention by miRNA on OA. Furthermore, aging-related transcriptional factor and SOX9 regulated the miRNA and host gene, and miRNA regulated several target genes.
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