Regulation of lineage fate in hematopoietic stem cells by Bach2(Fostering Joint International Research)
Project/Area Number |
15KK0328
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Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Tohoku University |
Principal Investigator |
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Research Collaborator |
McHeyzer-Williams Michael スクリプス研究所, Immunology and Microbiology, 教授
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Project Period (FY) |
2015 – 2017
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
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Keywords | 免疫記憶 / 獲得免疫 / 抗体産生 / 形質細胞 / メタロチオネイン / 亜鉛 / 長期生存細胞 / 1細胞RNA-Seq / 細胞長期生存 / 単一細胞遺伝子解析 / RNA-Seq / 長寿命 / 免疫学 / 単一細胞解析 |
Outline of Final Research Achievements |
Long-living plasma cells in bone marrow are key player of the memory of immunity. To elucidate the mechanism of long-living on plasma cells, we performed gene expression analysis using single cell RNA-sequencing (scRNA-seq). scRNA-seq analysis showed that MT1 and MT2 had high expression in bone marrow. These gene showed high correlation as expression level. We found that Hmox1 (HO-1) and Herped1, which are genes of anti-stress factors, highly expressing in MTs expressing cells in bone marrow. IL-6 stimulation increased MT1 and MT2 gene expression in spleen plasma cells. These result suggested that MT expressing cells have recieved survival factor stimulation in bone marrow.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] A Bach2-Cebp gene regulatory network for the commitment of multipotent hematopoietic progenitors.2017
Author(s)
Itoh-Nakadai, A., Matsumoto, M., Kato, H., Sasaki, J., Uehara, Y., Sato, Y., Ebina-Shibuya, R., Morooka, M., Funayama, R., Nakayama, K., Ochiai, K., Muto, A. and Igarashi, K.
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Journal Title
Cell Reports
Volume: 18
Issue: 10
Pages: 2401-2414
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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