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Molecular basis of the Turing model without diffusion

Research Project

Project/Area Number 15KT0079
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Constructive Systems Biology
Research InstitutionOsaka University

Principal Investigator

WATANABE Masakatsu  大阪大学, 生命機能研究科, 准教授 (90323807)

Research Collaborator USUI yuu  
KONDO shigeru  
Project Period (FY) 2015-07-10 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords反応拡散 / 色素細胞 / パターン形成 / ギャップ結合 / 形態形成 / 細胞間相互作用 / 細胞仮足 / Cytonemes / ゼブラフィッシュ
Outline of Final Research Achievements

The body shape of organisms depends on the arrangement of its constituent cells, and the arrangement sometimes is determined depending on the interaction between cells by cell autonomous manner. In order to elucidate the molecular mechanism of the cell-autonomous morphogenesis, we have analyzed the zebrafish skin pattern formation mechanism. The stripe on fish skin mainly consists of 2 types of cells; xanthophore and melanophore, and cell-cell interactions exist between these cells. In this study, as factors involved in this intercellular interaction, we analyzed the cell projections that black pigment cells extend, as well as connexins and Notch as molecular factors.

Academic Significance and Societal Importance of the Research Achievements

本研究は生物の形を決める分子メカニズムの解明を目指した基礎研究である。ゼブラフィッシュの体表模様を研究のモデルケースとし、黒色素細胞の集団サイズを規定するメカニズムの解明を目指している。今回の研究により、細胞集団サイズを決めるメカニズムの一つとして、ギャップ結合ネットワークの存在を示した。ギャップ結合は、、細胞間の低分子化合物の通過を制御し、細胞間相互作用に直接的に作用するチャネル分子である。更には、このネットワークには細胞間の接触が必要であり、これを仲介する細胞仮足の上に成り立つこととNotchシグナルが相補的に作用していることを示した。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (3 results)

  • [Journal Article] KLF4-Induced Connexin40 Expression Contributes to Arterial Endothelial Quiescence.2019

    • Author(s)
      Denis, J. F., Diagbouga, M. R., Molica, F., Hautefort, A., Linnerz, T., Watanabe, M., Lemeille, S., Bertrand, J. Y., and Kwak, B. R
    • Journal Title

      Front Physiol

      Volume: 10 Pages: 80-80

    • DOI

      10.3389/fphys.2019.00080

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Melanophore multinucleation pathways in zebrafish2018

    • Author(s)
      Usui Y, Kondo S, Watanabe M
    • Journal Title

      Dev Growth Differ.

      Volume: 2018 Aug 7 Issue: 7 Pages: 454-459

    • DOI

      10.1111/dgd.12564

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Connexin Communication Compartments and Wound Repair in Epithelial Tissue2018

    • Author(s)
      Chanson, M., Watanabe, M., O'Shaughnessy, E. M., Zoso, A., and Martin, P. E
    • Journal Title

      Int J Mol Sci

      Volume: 19(5) Issue: 5 Pages: 1354-1354

    • DOI

      10.3390/ijms19051354

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gap Junction in the Teleost Fish Lineage: Duplicated Connexins May Contribute to Skin Pattern Formation and Body Shape Determination2017

    • Author(s)
      Watanabe, M
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 21 February

    • DOI

      10.3389/fcell.2017.00013

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ゼブラフィッシュのチューリングパターン形成メカニズムの解析2017

    • Author(s)
      渡邉正勝
    • Journal Title

      実験医学増刊号

      Volume: 3 Pages: 192-194

    • Related Report
      2016 Research-status Report
  • [Journal Article] Two Different Functions of Connexin43 Confer Two Different Bone Phenotypes in Zebrafish2016

    • Author(s)
      Misu, A., Yamanaka, H., Aramaki, T., Kondo, S., Skerrett, I. M., Iovine, M. K. & Watanabe, M
    • Journal Title

      J Biol Chem

      Volume: 291 Issue: 24 Pages: 12601-12611

    • DOI

      10.1074/jbc.m116.720110

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Genetics of body shape: Connexin43 is the key to two zebrafish mutants with shorter backbones and fins2016

    • Author(s)
      Masakatsu Watanabe and Shigeru Kondo
    • Journal Title

      Atlas of Science

      Volume: 26 Sep

    • Related Report
      2016 Research-status Report
    • Open Access
  • [Presentation] Melanophore multinucleation pathways in zebrafish2018

    • Author(s)
      Yuu Usui, Shigeru Kondo, and 〇Masakatsu Watanabe
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 黒色素細胞の多核化について2018

    • Author(s)
      臼居優、近藤滋、〇渡邉正勝
    • Organizer
      日本動物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Study of gap junction circuits in pigment cells that regulate sizes of melanophore population in zebrafish pattern formation2017

    • Author(s)
      Usui, Y, Aramaki, T, Kondo, S, Watanabe,M
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Research-status Report

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Published: 2015-07-14   Modified: 2021-01-27  

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