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Development of single particle electron microscopy

Research Project

Project/Area Number 16087210
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

SATO Chikara  National Institute of Advanced Industrial Science and Technology, 脳神経情報研究部門, 研究グループ長 (00357146)

Co-Investigator(Kenkyū-buntansha) KAWATA Masaaki  独立行政法人産業技術総合研究所, 情報技術研究部門, 主任研究員 (20356843)
OGURA Toshihiko  独立行政法人産業技術総合研究所, 脳神経情報研究部門, 主任研究員 (70371028)
MIO Kazuhiro  独立行政法人産業技術総合研究所, バイオメディシナル情報センター, 主任研究員 (40470041)
Project Period (FY) 2004 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥76,900,000 (Direct Cost: ¥76,900,000)
Fiscal Year 2009: ¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2008: ¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 2007: ¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2006: ¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2005: ¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 2004: ¥8,300,000 (Direct Cost: ¥8,300,000)
Keywordsタンパク質 / 3次元構造決定 / 電子顕微鏡 / ナノテクノロジー / 画像情報処理 / ニューラルネット / グリッドコンピューティング / クライオ電顕 / ニューラルネットワーク / グリッドコンピューティンク / 蛋白質 / ナノバイオ / 画像、文章、音声認識等 / 神経科学
Research Abstract

Single particle 3D reconstruction is a method produced bycombining biochemistry, electron microscopy and image analysis. To improve theresolution and automation of single particle reconstruction (SPR), we have introducedsimulated annealing (SA) to SPR, and developed it. These methods were first applied to theion channels, such as TRPC3, TRPM2 and Orai1, then to CFTR ion pump Prestin, carcinogenesis related keap1, and further to Alzheimer disease related γ-secretase.

Report

(8 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Products Report
  • Research Products

    (92 results)

All 2013 2012 2010 2009 2008 2007 2006 2005 2004 Other

All Journal Article (40 results) (of which Peer Reviewed: 29 results) Presentation (43 results) Remarks (1 results) Patent(Industrial Property Rights) (8 results) (of which Overseas: 2 results)

  • [Journal Article] Keap1 is a forked-stem dimer struct ure with two large spheres enclosin g the intervening, double glycine r epeat, and C-terminal domains2010

    • Author(s)
      T. Ogura, K.I. Tong, K. Mio, Y. Maruyam a, H. Kurokawa, C. Sato, M. Yamamoto.
    • Journal Title

      Proc. Natl. Acad. Sci. U S A 107

      Pages: 2842-2847

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Atmospheric scanning elect ron microscope observes cells and t issues in open medium through silic on nitride film2010

    • Author(s)
      H. Nishiyama, M. Suga, T. Ogura, Y. Mar uyama, M. Koizumi, K. Mio, S. Kitamura, C. Sato.
    • Journal Title

      J Struct. Biol. 169

      Pages: 438-449

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Keapl is a forked-stem dimer structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminaI domains.2010

    • Author(s)
      T.Ogura
    • Journal Title

      Proc. Natl.Acad.Sci.USA VOL.107

      Pages: 2842-2847

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.2010

    • Author(s)
      H.Nishiyama
    • Journal Title

      J.Struct.Biol. VOL.169

      Pages: 438-449

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetrameric orai1 is a teard rop-shaped molecule with a long, ta pered cytoplasmic domain2009

    • Author(s)
      Y. Maruyama, T. Ogura, K. Mio, K. Kato, T. Kaneko, S. Kiyonaka, Y. Mori, C. Sato
    • Journal Title

      J. Biol. Chem. 284(20)

      Pages: 13676-13685

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective and direct i nhibition of TRPC3 channels underli es biological activities of a pyraz ole compound2009

    • Author(s)
      S. Kiyonaka, K. Kato, M. Nishida, K. Mi o, T. Numaga, Y. Sawaguchi, T. Yoshida, M. Wakamori, E. Mori, T. Numata, M. Is hii, H. Takemoto, A. Ojida, K. Watanab e, A. Uemura, H. Kurose, T. Morii, T. K obayashi, Y. Sato, C. Sato, I. Hamachi, Y. Mori
    • Journal Title

      PNAS 106(13)

      Pages: 5400-5405

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 大気圧走査電子顕微鏡(ASEM)による溶液中での細胞観察2009

    • Author(s)
      西山英利
    • Journal Title

      顕微鏡 44巻

      Pages: 262-267

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atmospheric Electron Microscope : Limits of Observable Depth.2009

    • Author(s)
      M.Suga
    • Journal Title

      Microsc Microanal VOL.15

      Pages: 924-925

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New Scanning Electron Microscope Capable of Observing Cells in Solution.2009

    • Author(s)
      H.Nishiyama
    • Journal Title

      Microsc Microanal VOL.15

      Pages: 938-939

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 結晶を用いない電子顕微鏡画像からの3次元構造決定:単粒子解析法2009

    • Author(s)
      三尾和弘
    • Journal Title

      化学と生物 47巻

      Pages: 711-717

    • NAID

      10026436693

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reply to Thinnes : Is There competition in Trafficking of VDAC-cored VRAC and SOC in NE Differentiation of Cells.2009

    • Author(s)
      C.Sato
    • Journal Title

      J.Biol.Chem. VOL.284

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tetrameris orai1 is a teardrop-shaped molecule with a long, tapered cytoplasmic domain.2009

    • Author(s)
      Y.Maruyama
    • Journal Title

      J.Biol.Chem. VOL.284

      Pages: 13676-13685

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound.2009

    • Author(s)
      S.Kiyonaka
    • Journal Title

      PNAS VOL.106

      Pages: 5400-5405

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of the P2X_2 receptor revealed a vase-shaped structure with lateral tunnels above the membrane2009

    • Author(s)
      K. Mio
    • Journal Title

      Structure 117

      Pages: 266-275

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RECK forms cowbell-shaped dimers and inhibits MMP catalyzed cleavage of fibronectin2009

    • Author(s)
      A. Omura
    • Journal Title

      J. Biol. Chem. 284

      Pages: 3461-3469

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 単粒子解析法によるセンサーチャネルタンパク質の解析(MS36)2009

    • Author(s)
      三尾和弘
    • Journal Title

      日本結晶学会誌 51

      Pages: 71-72

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional reconstruction of Human CFTR chloride channel revea led an ellipsoidal structure with o rifices beneath the putative tran smembrane domain2008

    • Author(s)
      K. Mio, T. Ogura, M. Mio, H. Shimizu, TC. Hwang, C. Sato, Y. Sohma
    • Journal Title

      J. Biol. Chem. 283(44)

      Pages: 30300-30310

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The motor prote in prestin is a bullet-shaped molec ule with inner cavities2008

    • Author(s)
      K. Mio, Y. Kubo, T. Ogura, T. Yamamoto, F. Arisaka, C. Sato
    • Journal Title

      J. Biol. Chem. 283(2)

      Pages: 1137-1145

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional reconstruction of human CFTR chloride channel revealed an ellipsoidal structure with orifices beneath the putative trans membrane domain2008

    • Author(s)
      K. Mio
    • Journal Title

      J. Biol. Chem. 283

      Pages: 30300-30310

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 電顕画像からの自動3次元再構成法の開発とTRPチャネルの構造2008

    • Author(s)
      小椋俊彦
    • Journal Title

      顕微鏡 43

      Pages: 40-45

    • NAID

      10021151773

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The motor protein prestin is a bullet-shaped molecule with inner cavities2008

    • Author(s)
      K.Mio
    • Journal Title

      J.Biol.Chem 283

      Pages: 1137-1145

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of six-transmembrane cation channels revealed by single-paxticle analysis from EM images2008

    • Author(s)
      K.Mio
    • Journal Title

      J.Synchrotron Radiation 15

      Pages: 211-214

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Threedimensional reconstruction using tr ansmission EM reveals a swollen, be ll-shaped structure of TRPM2 cation channel2007

    • Author(s)
      Y. Maruyama, T. Ogura, K. Mio, S. Kiyon aka, K. Kato, Y. Mori, C. Sato
    • Journal Title

      J. Biol. Chem. 282(51)

      Pages: 36961-36970

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRIC channels are essential for Ca2+ handling in intracellular stores2007

    • Author(s)
      M. Yazawa, C. Ferrante, J. Feng, K. Mio, T. Ogura, M. Zhang, P-H. Lin, Z. Pan, S. Komazaki, K. Kato, M. Nishi, X. Zhao, N. Weisleder, C. Sato, J. Ma, H. Tak eshima
    • Journal Title

      Nature 448(7149)

      Pages: 78-82

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional reconstruction using transmission EM reveals a swollen,bell-shaped structure of TRPM2 cation channel2007

    • Author(s)
      Y.Maruyama
    • Journal Title

      J.Biol.Chem 282

      Pages: 36961-36970

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRIC channels are essential for Ca^2+handling in intracellular stores2007

    • Author(s)
      M.Yazawa
    • Journal Title

      Nature 448

      Pages: 78-82

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A statistically harmonized alignment-classification in image space enables accurate and robust alignment of noisy images in single particle analysis2007

    • Author(s)
      M.Kawata
    • Journal Title

      J.Electron Microscopy 56

      Pages: 83-92

    • NAID

      10028200131

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Subunit dissociation of TRPC3 ion channel under high-salt condition2007

    • Author(s)
      K.Mio
    • Journal Title

      J.Electron Microscopy 56

      Pages: 111-117

    • NAID

      10028200220

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas2007

    • Author(s)
      K.Mio
    • Journal Title

      J. Mol. Biol. 367・2

      Pages: 373-383

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A fully automat ic 3D reconstruction method using s imulated annealing enables accurat e posterioric angular assignment of protein projections2006

    • Author(s)
      T. Ogura, C. Sato
    • Journal Title

      J. Struct. Biol. 156

      Pages: 371-386

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A fully automatic 3D reconstruction method using simulated annealing enables accurate posterioric angular assignment of protein proiections2006

    • Author(s)
      T.Ogura
    • Journal Title

      J. Struct. Biol. 156・3

      Pages: 371-386

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Three-dimensional structure of the g-secretase complex2006

    • Author(s)
      T.Ogura
    • Journal Title

      Biochem.Biophys.Res.Comm. 343・2

      Pages: 525-534

    • Related Report
      2005 Annual Research Report
  • [Journal Article] イオンチャンネル・受容体の単粒子構造解析2006

    • Author(s)
      三尾和弘
    • Journal Title

      細胞工学 25・3

      Pages: 236-241

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Visualization of the trimeric P2X2 receptor with a crown-capped extracellular domain2005

    • Author(s)
      K.Mio
    • Journal Title

      Biochem.Biophys.Res.Comm. 337・3

      Pages: 998-1005

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end2005

    • Author(s)
      K.Mio
    • Journal Title

      Biochem.Biophys.Res.Comm. 333・3

      Pages: 768-777

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ナトリウムチャンネルの電子顕微鏡単粒子構造解析2005

    • Author(s)
      小椋俊彦
    • Journal Title

      蛋白質核酸酵素 5・10

      Pages: 1284-1291

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 結晶を用いない電子顕微鏡構造解析:単粒子解析法による構造解明2005

    • Author(s)
      小椋俊彦
    • Journal Title

      遺伝子医学MOOK別冊/分子生物学実験シリーズ 図・写真で観るタンパク構造・機能解析実験実践ガイド

      Pages: 101-108

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 電子顕微鏡を用いた単粒子解析法によるIP3受容体チャンネルの構造解明2004

    • Author(s)
      佐藤主税
    • Journal Title

      AIST Today 9

      Pages: 18-18

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A statistically harmonized alignment-classification in image space enables accurate and robust alignment of noisy images in single particle analysis

    • Author(s)
      M.Kawata
    • Journal Title

      J. Electron Microscopy. (in press)

    • NAID

      10028200131

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Subunit dissociation of TRPC3 ion channel under high-salt condition

    • Author(s)
      K.Mio
    • Journal Title

      J. Electron Microscopy. (in press)

    • NAID

      10028200220

    • Related Report
      2006 Annual Research Report
  • [Presentation] 新しい大気圧走査電子顕微鏡を用いた細胞の高速観察の可能性2010

    • Author(s)
      須賀三雄
    • Organizer
      第83回 日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 環境対応型SEMとその応用2010

    • Author(s)
      西山英利
    • Organizer
      日本顕微鏡学会 第34回関東支部講演会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] New Atmospheric Scanning Electron Microscope observes cells in solution as an optical-and electron-correlative microscope2010

    • Author(s)
      M.Suga
    • Organizer
      The 5th international Conference on Structural Analusis of Supramolecular Assenmblies by Hybrid Methods
    • Place of Presentation
      Lake Tahoe, USA
    • Year and Date
      2010-03-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] The Atmospheric Sem Observ es Nuclei in the Surface Level of T issue Without Thin-Sectioning2009

    • Author(s)
      C. Sato
    • Organizer
      The American Society for Cell Biology 4 9th Annual Meeting
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2009-12-08
    • Related Report
      2009 Final Research Report
  • [Presentation] The Atmospheric Sem Observes Nuclei in the Surface Level of Tissue Without Thin-Sectioning.2009

    • Author(s)
      C.Sato
    • Organizer
      The American Society for Cell Biology 49th Annual Meeting
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2009-12-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] The New Atmospheric Scanning Electron Microscope2009

    • Author(s)
      H.Nishiyama
    • Organizer
      The American Society for Cell Biology 49th Annual Meeting
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2009-12-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] The New Atomospheric Scanning Electron Microscope Observes Cells in Solution as Optical-and Electron Correlative Microscope2009

    • Author(s)
      M.Suga
    • Organizer
      The American Society for Cell Biology 49th AnnualMeeting
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2009-12-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 液中金属析出の大気圧走査電子顕微鏡観察2009

    • Author(s)
      小入羽祐治
    • Organizer
      第50回 電池討論会
    • Place of Presentation
      京都
    • Year and Date
      2009-12-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] TEMとSEMによるタンパク質複合体の構造解析2009

    • Author(s)
      佐藤主税
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      徳島市
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Three-dimensional structu res of ion channels revealed by ele ctron microscopy: sodium, TRP, CFTR and Orai1 channels2009

    • Author(s)
      C. Sato
    • Organizer
      Neuroscience 2 009
    • Place of Presentation
      シカゴ
    • Year and Date
      2009-10-21
    • Related Report
      2009 Final Research Report
  • [Presentation] Three-dimensional structures of ion channels revealed by electron microscopy : sodium, TRP, CFTR and Orail channels.2009

    • Author(s)
      Chikara Sato
    • Organizer
      Neuroscience 2009
    • Place of Presentation
      シカゴ
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] Atmospheric Scanning Electron Microscope (ASEM) : Observation of cellsin medium2009

    • Author(s)
      H.Nishiyama
    • Organizer
      Neuroscience 2009
    • Place of Presentation
      シカゴ
    • Year and Date
      2009-10-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Electron microscopy as a tool for determining protein 3D structure related to channel diseases and metastasis.2009

    • Author(s)
      Chikara Sato
    • Organizer
      14th World Congress on Advances in Oncology and 12th International Symposium on Molecular Medicine
    • Place of Presentation
      Loutraki Greese
    • Year and Date
      2009-10-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Biomedicinal Information Research Center Protein Structural Information Analysis Team.2009

    • Author(s)
      Kazuhiro Mio
    • Organizer
      14th World Congress on Advances in Oncology and 12th International Symposium on Molecular Medicine
    • Place of Presentation
      Loutraki Greese
    • Year and Date
      2009-10-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 結晶を用いない単粒子解析法によるイオンチャンネルの構造解析2009

    • Author(s)
      佐藤主税
    • Organizer
      第32回日本神経科学学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 単粒子電顕構造解析法とSEMによる分子・細胞レベルの観察.2009

    • Author(s)
      三尾和弘
    • Organizer
      生理学研究所研究会「作動中の膜機能分子の姿を捉える-静止画から動画ヘ-」
    • Place of Presentation
      岡崎
    • Year and Date
      2009-09-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Orailチャネル三次元構造の解明2009

    • Author(s)
      佐藤主税
    • Organizer
      第5回TRPチャネル研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 電子顕微鏡による膜蛋白質やその複合体等の構造解析.2009

    • Author(s)
      佐藤主税
    • Organizer
      日本顕微鏡学会第65回学術講演会
    • Place of Presentation
      仙台
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大気圧走査電子顕微鏡はどこまで深く見えているか.2009

    • Author(s)
      須賀三雄
    • Organizer
      日本顕微鏡学会第65回学術講演会
    • Place of Presentation
      仙台
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 溶液中における細胞の観察を可能にする大気圧走査電子顕微鏡.2009

    • Author(s)
      西山英利
    • Organizer
      日本顕微鏡学会第65回学術講演会
    • Place of Presentation
      仙台
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Three dimensional structures of ion channels revealed by electronmicroscopy : Na channel, IP_3 receptor, TRP channels and gamma-secretase2009

    • Author(s)
      佐藤主税
    • Organizer
      2009 IUPS International Conference of Physiological Sciences meeting
    • Place of Presentation
      Busan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] センサー膜タンパク質の構造解明12009

    • Author(s)
      三尾和弘
    • Organizer
      2008年度 日本顕微鏡学会 生体構造解析分科会 研究討論会VIII
    • Place of Presentation
      別府
    • Year and Date
      2009-01-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] センサー膜タンパク質の構造解明22009

    • Author(s)
      佐藤主税
    • Organizer
      2008年度 日本顕微鏡学会 生体構造解析分科会 研究討論会VIII
    • Place of Presentation
      別府
    • Year and Date
      2009-01-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 結晶を用いない単粒子電顕解析法によるイオンチャネルの構造解析 : Na・TRP・IP3Rチャネルの構造比較2008

    • Author(s)
      佐藤主税
    • Organizer
      BMB2008(第31回目本分子生物学会年会 第81回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 電圧感受型モータータンパク質プレスチンの電子顕微鏡を用いた構造解析2008

    • Author(s)
      三尾和弘
    • Organizer
      BMB2008(第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 培養液中における細胞の走査型電子顕微鏡観察2008

    • Author(s)
      西山英利
    • Organizer
      BMB2008(第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Maximum likelihood appr oach for picking-up process in sing le particle analysis2008

    • Author(s)
      M. Kawata
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Maximum likelihood approach for picking-up process in single particle analysis2008

    • Author(s)
      川田正晃
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 単粒子解析によるTRP channe l family間での構造比較. Structure of TRP channels revealed by single particle analysis using EM images2008

    • Author(s)
      M. Kawata
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-03
    • Related Report
      2009 Final Research Report
  • [Presentation] 単粒子解析によるTRP channel family間での構造比較(Structure of TRP channels revealed by single particle analysis using EM images)2008

    • Author(s)
      佐藤主税
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] 電圧感受型モータータンパク質プレスチンの電子顕微鏡を用いた構造解析2008

    • Author(s)
      三尾和弘
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] The 3D structure of ion c hannels, sensors and receptors reve aled by electron microscopy2008

    • Author(s)
      T. Ogura
    • Organizer
      Inter national Conference of Physiologica l Sciences and 11th International S ymposium on Molecular Medicine, Cr ete
    • Place of Presentation
      Greece
    • Year and Date
      2008-10-09
    • Related Report
      2009 Final Research Report
  • [Presentation] The 3D structure of ion channels, sensors and receptors revealed by electron microscopy2008

    • Author(s)
      佐藤主税
    • Organizer
      International Conference of Physiological Sciences and 11th International Symposium on Molecular Medicine
    • Place of Presentation
      Crete
    • Year and Date
      2008-10-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Ion channel structures by single particle analysis using EM : Sodium and TRP channels, IP3 receptor2008

    • Author(s)
      佐藤主税
    • Organizer
      IUCr2008
    • Place of Presentation
      大阪
    • Year and Date
      2008-08-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] TRPM2 channelの単粒子解析による構造解明2008

    • Author(s)
      佐藤主税
    • Organizer
      第4回TRPチャネル研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2008-06-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 単粒子解析法によるイオンチャンネル3次元構造の解明2008

    • Author(s)
      佐藤 主税
    • Organizer
      岡崎統合バイオサイエンスセンターシンポジウム
    • Place of Presentation
      岡崎
    • Year and Date
      2008-03-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] TRPC3の3次元構造2007

    • Author(s)
      佐藤 主税
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-12-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] New robust method to create finer two-dimensional average imares in single particle analysis2007

    • Author(s)
      川田 正晃
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-12-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] 単粒子解析によるイオンチャンネルの再構成2007

    • Author(s)
      佐藤 主税
    • Organizer
      日本顕微鏡学会生体構造解析分科会
    • Place of Presentation
      東京
    • Year and Date
      2007-12-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] Ion channel structure revealed by single particle analysis of EM image2007

    • Author(s)
      佐藤 主税
    • Organizer
      ISDSB2007
    • Place of Presentation
      東京
    • Year and Date
      2007-09-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] 単粒子解析法によるイオンチャネルの構造解明2007

    • Author(s)
      佐藤 主税
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2007-09-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] TRPC3 channelの単粒子解析による構造解明とそこから推測される機構2007

    • Author(s)
      佐藤 主税
    • Organizer
      第3回 TRPチャネル研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2007-07-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] 単粒子解析によるイ牙シチャンネルの構造解明2007

    • Author(s)
      佐藤 主税
    • Organizer
      日本顕微鏡学会第63回学術講演会
    • Place of Presentation
      新潟
    • Year and Date
      2007-05-21
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://unit.aist.go.jp/neurosci/japanese/group/kouzouseiri.html

    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] STRUCTURE DETERMINATION SYSTEM, STRUCTURE DETERMINATION METHOD, AND PROGRAM2013

    • Inventor(s)
      佐藤 主税
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2013-03-05
    • Related Report
      Products Report
    • Overseas
  • [Patent(Industrial Property Rights)] 画像処理システム、画像処理方法、プログラムおよび記録媒体2012

    • Inventor(s)
      佐藤 主税, 川田 正晃
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2012-01-13
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] 構造推定システム、構造推定方法およびプログラム2012

    • Inventor(s)
      佐藤 主税, 川田 正晃
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2012-01-06
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] 構造推定システム、構造推定方法およびプログラム2010

    • Inventor(s)
      川田正晃、佐藤主税
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Filing Date
      2010-03-18
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 画像処理システム、画像処理方法、プログラムおよび記録媒体2008

    • Inventor(s)
      川田正晃、佐藤主税
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Number
      2008-289005
    • Filing Date
      2008-11-11
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 構造推定システム、構造推定方法およびプログラム2008

    • Inventor(s)
      川田正晃、佐藤主税
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Filing Date
      2008-08-22
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 電子顕微鏡観察像の画像処理方法および画像処理プログラム並びに記録媒体2007

    • Inventor(s)
      小椋俊彦、佐藤主税、藤吉好則
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Acquisition Date
      2007-06-15
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 構造推定システム、構造推定方法およびプログラム2007

    • Inventor(s)
      川田 正晃, 佐藤 主税
    • Industrial Property Rights Holder
      独立行政法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2007-10-05
    • Related Report
      Products Report
    • Overseas

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Published: 2004-04-01   Modified: 2018-03-28  

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