Analysis of target molecules and their effective carbohydrates for Helicobacter pyrori
| Project/Area Number |
16570101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokai University |
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Principal Investigator |
KOJIMA Naoya Tokai University, Future Science and Technology Joint Research Center, Professor, 未来科学技術共同研究センター, 教授 (30183338)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Helicobacter pylori / Microdomain / Glycolipid |
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| Research Abstract |
Recently, the microdomain on cell surface is considered to be important for cell signaling. In order to investigate the receptor molecules for Helicobacter pylori, this study involved the analysis of adhesion of H.pylori tomicrodomain of a human gastric cancer cell line, KATO III. The microdomain was isolated as low density fractions from the homogenate of KATO III cells by means of sucrose density gradient centrifugation. In the direct adhesion experiment, Helicobacter pylori clearly adhered to the low density fraction, suggesting that the glycosphingolipids contained in the microdomain is involved in the adhesion of the bacteria. Then, we tried to separate the glycolipid. The glycolipids were extracted from the domain, and separated to neutral and acidic gllycolipids by DEAE-Sehadex. The bacteria adhered to the neutral glycolipid fraction strongly, indicating that a part of neutral glycolipids is a candidate for the receptor of Helicobacter pylori. Therefore, we tried to identify the receptor glycolipid.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Novel Role of the Ras Cascade in Memory B Cell Response2005
Author(s)
Takahasi, Y., Inamine, A., Hoshimoto, S., Haraguchi, S., Yoshizawa, E., Kojima, N., Abe, R., Takemori, T
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Journal Title
Immunity 23
Pages: 127-138
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[Journal Article] Histochemical Expression of Siloglycoconjugates in Carcinoma of the Papilla of Vater.2005
Author(s)
Tang W, Guo Q, Usuda M, Kokudo N, Seyama Y, Minagawa M, Sugawara Y, Nakata, M., Kojima, N., Makuuchi, M
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Journal Title
Hepato-Gastroenterology 52
Pages: 67-71
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[Patent(Industrial Property Rights)] 特許2005
Inventor(s)
小島 直也, 池原譲, 辻村邦夫
Industrial Property Rights Holder
東海大学, 愛知県
Industrial Property Number
2005-094380
Filing Date
2005-03-29
Description
「研究成果報告書概要(和文)」より
Related Report
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