| Project/Area Number |
16590314
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National University Corporation Tottori University |
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Principal Investigator |
HAYASHI Kazuhiko Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (30180962)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Masao Okayama University, Dentisty and Pharmaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (40166731)
SAIRENJI Takeshi Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (10117351)
HORIE Yasushi Tottori University, University Hospital, Associate professor, 医学部附属病院, 助教授 (60229226)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | EBV / Herpesvirus papio / Rabbit / Hemophagocytic Syndrome / LPD / T-cell |
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| Research Abstract |
Epstein-Barr virus associated hemophagocytic syndrome (EBV-AHS) is often associated with fatal infectious mononucleosis or T -cell lymphoproliferative diseases (LPD). To elucidate the pathogenesis of fatal Herpesvirus papio (HVP)-infected LPD and subsequently developed HVP-negative lymphomas observed in HVP-induced rabbit hemophagocytic syndrome, we analyzed sequential examination by ultrasonography (US), CT,CBC, antibody titers, viral quantification, liver biopsy of HVP-induced rabbit LPD and lymphoma development. All of the nine Japanese White rabbits inoculated intravenously with HVP died of fatal LPD or lymphomas. LPD was often accompanied by hemophagocytic syndrome (HPS). Sequential laboratory examinations revealed the increase or decrease of anti-EBV-VCA-IgG titers and viral roads. US or CT examination showed the continuously enlarged splenomegaly. Liver biopsy revealed the transient HVP-EBER-1-positive atypical lymphocyte infiltration. Autopsy examination reconfirmed the development of HVP-EBER-1-negative lymphomas in 2 infected rabbits with long survival and HVP-infected LPD in all cases. Hemophagocytic histiocytosis was observed in the lymph nodes, spleen, bone marrow, or thymus. HVP-DNA was detected in the tissues and peripheral blood from the infected rabbits by PCR or Southern blot analysis. These data suggest that HVP-negative lymphomas developed among HVP-positive LPD may be the good model for "hit and run" oncogenesis theory by herpesvirus.
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