| Budget Amount *help |
¥41,210,000 (Direct Cost: ¥31,700,000、Indirect Cost: ¥9,510,000)
Fiscal Year 2018: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2017: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2016: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
|
|---|
| Outline of Final Research Achievements |
Exosomal microRNA (miRNA) expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable microdevices for multiple RNA expression analysis by miRNA purification and qRT-PCR for point-of-care testing. These microdevices are composed of several types of modules termed ‘chemical IC chips’. The developed microdevice was the palm size, and improved the portability of all systems. For the practical use, the microdevice also improved the disposability. The microdevice could perform reactions for samples of small volume. We purified miRNA from the liver cancer cell line and confirmed the expression level of miRNA, which is a potential biomarker for liver cancer, by the microdeivces. We observed an accurate reaction in the microdevice. We also demonstrated that our microdevice can detect miRNA cancer biomarkers at the early stage.
|
