Micro-nano droplet handling for the measurement of biological samples
| Project/Area Number |
16H02349
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Measurement engineering
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| Research Institution | Waseda University |
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Principal Investigator |
SHOJI Shuichi 早稲田大学, 理工学術院, 教授 (00171017)
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| Co-Investigator(Kenkyū-buntansha) |
竹山 春子 早稲田大学, 理工学術院, 教授 (60262234)
水野 潤 早稲田大学, ナノ・ライフ創新研究機構, 上級研究員(研究院教授) (60386737)
関口 哲志 早稲田大学, ナノ・ライフ創新研究機構, 上級研究員(研究院教授) (70424819)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2018: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2017: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2016: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
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| Keywords | 計測システム / 計測工学 |
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| Outline of Final Research Achievements |
In this research project, we defined required specs and established fundamental technologies for “handling of micro samples” in micro scale channels. We developed following technologies through this research, 1) stable and reproducible micro droplets generation ranged from several microns to sub-micron in diameter, 2) sample collection into a micro droplet and/or control of chemical reaction in the micro droplets, 3) selective handling of the specific micro droplet. In addition to these achievements, we developed bendable ITO electrodes for flexible electronic devices as well as basic technologies to realize “Integrated Gene Logic-Chip”.
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| Academic Significance and Societal Importance of the Research Achievements |
従来は、多数の同種のサンプルの中から特殊な機能を発現したサンプル（細胞・細菌・化学物質等）だけをピックアップし、さらにそれらを培養・観察してその機能解析を行う事や、分解・抽出してさらにこれを利用するためには、必要な作業を手動で行う以外方法は無かった。本研究の成果により可能となった、マイクロ流体デバイスを利用した微小液滴の作製と、その受動的な微小流れの制御を応用することにより、特定の微小サンプルをピックアップして取り扱う、システムとしての自動制御に道が開かれた。
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Construction of integrated gene logic-chip2018
Author(s)
Takeya Masubuchi, Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama, Yoshie Harada, Takuya Ueda, Hisashi Tadakuma
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Journal Title
Nat. Nanotechnol.
Volume: 13
Issue: 10
Pages: 933-940
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array2016
Author(s)
M. Isokawa, K. Takatsuki, Y.T. Song, K. Shih, K. Nakanishi, Z. Xie, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji,
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Journal Title
Analytical Chemistry
Volume: 88
Issue: 12
Pages: 64856491-64856491
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] A Micromachined Liquid Chromatography Chip with a Pillar Array Mixer for Post-Column Derivatization in the Analysis of Neurotransmitters2017
Author(s)
M. Isokawa, K. Nakanishi, T. Kanamori, H. Zhuang, H. Yamazaki, T. Sugaya, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
Organizer
The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2017)
Related Report
Int'l Joint Research
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[Presentation] In Vitro Selection of Novel Peptide Agonists for Human Somatostatin Receptor Subtype-2 Using a Water-In-Oil Microdroplet Platform2016
Author(s)
T. Sakurai, R. Iizuka, Y. Nakamura, J. Ishii, A. Kondo, A. Iguchi, D.H. Yoon, T. Sekiguchi, S. Shoji, T. Funatsu
Organizer
The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Presentation
Dublin, Ireland
Year and Date
2016-10-09
Related Report
Int'l Joint Research
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