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Micro-nano droplet handling for the measurement of biological samples

Research Project

Project/Area Number 16H02349
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Measurement engineering
Research InstitutionWaseda University

Principal Investigator

SHOJI Shuichi  早稲田大学, 理工学術院, 教授 (00171017)

Co-Investigator(Kenkyū-buntansha) 竹山 春子  早稲田大学, 理工学術院, 教授 (60262234)
水野 潤  早稲田大学, ナノ・ライフ創新研究機構, 上級研究員(研究院教授) (60386737)
関口 哲志  早稲田大学, ナノ・ライフ創新研究機構, 上級研究員(研究院教授) (70424819)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2018: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2017: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2016: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Keywords計測システム / 計測工学
Outline of Final Research Achievements

In this research project, we defined required specs and established fundamental technologies for “handling of micro samples” in micro scale channels. We developed following technologies through this research, 1) stable and reproducible micro droplets generation ranged from several microns to sub-micron in diameter, 2) sample collection into a micro droplet and/or control of chemical reaction in the micro droplets, 3) selective handling of the specific micro droplet. In addition to these achievements, we developed bendable ITO electrodes for flexible electronic devices as well as basic technologies to realize “Integrated Gene Logic-Chip”.

Academic Significance and Societal Importance of the Research Achievements

従来は、多数の同種のサンプルの中から特殊な機能を発現したサンプル(細胞・細菌・化学物質等)だけをピックアップし、さらにそれらを培養・観察してその機能解析を行う事や、分解・抽出してさらにこれを利用するためには、必要な作業を手動で行う以外方法は無かった。本研究の成果により可能となった、マイクロ流体デバイスを利用した微小液滴の作製と、その受動的な微小流れの制御を応用することにより、特定の微小サンプルをピックアップして取り扱う、システムとしての自動制御に道が開かれた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (42 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (15 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 15 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (20 results) (of which Int'l Joint Research: 17 results) Patent(Industrial Property Rights) (6 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] 釜山大学校(韓国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Construction of integrated gene logic-chip2018

    • Author(s)
      Takeya Masubuchi, Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama, Yoshie Harada, Takuya Ueda, Hisashi Tadakuma
    • Journal Title

      Nat. Nanotechnol.

      Volume: 13 Issue: 10 Pages: 933-940

    • DOI

      10.1038/s41565-018-0202-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Stochastic Expression of Lactate Dehydrogenase A Induces Escherichia Coli Persister Formation2018

    • Author(s)
      N. Yamamoto, R. Isshiki, Y. Kawai, D. Tanaka, T. Sekiguchi, S. Matsumoto, S. Tsuneda
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 126 Issue: 1 Pages: 30-37

    • DOI

      10.1016/j.jbiosc.2018.01.016

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Formation of Oil-in-Water (O/W) and Water-in-Oil-in-Water (W/O/W) Droplets in PDMS Device Using Protrusion Channel without Hydrophilic Surface Treatment2018

    • Author(s)
      D.H. Yoon, D. Tanaka, T. Sekiguchi, S. Shoji
    • Journal Title

      Micromachines

      Volume: 9 Issue: 9 Pages: 468-468

    • DOI

      10.3390/mi9090468

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Size-Dependent and Property-Independent Passive Microdroplet Sorting by Droplet Transfer on Dot Rails2018

    • Author(s)
      D.H. Yoon, D. Tanaka, T. Sekiguchi, S. Shoji
    • Journal Title

      Micromachines

      Volume: 9 Issue: 10 Pages: 513-513

    • DOI

      10.3390/mi9100513

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Color-tunable microfluidic electrogenerated chemiluminescence cells using Y-shaped micromixer2018

    • Author(s)
      Kasahara Takashi、Ishimatsu Ryoichi、Kuwae Hiroyuki、Shoji Shuichi、Mizuno Jun
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 57 Issue: 12 Pages: 128001-128001

    • DOI

      10.7567/jjap.57.128001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical Reinforcement of Low-Concentration Alginate Fibers by Microfluidic Embedding of Multiple Cores2018

    • Author(s)
      Yoon Dong Hyun、Tanaka Daiki、Sekiguchi Tetsushi、Shoji Shuichi
    • Journal Title

      Macromolecular Materials and Engineering

      Volume: 303 Issue: 3 Pages: 1700516-1700516

    • DOI

      10.1002/mame.201700516

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly flexible transparent electrodes based on mesh-patterned rigid indium tin oxide2018

    • Author(s)
      Sakamoto Kosuke、Kuwae Hiroyuki、Kobayashi Naofumi、Nobori Atsuki、Shoji Shuichi、Mizuno Jun
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-8

    • DOI

      10.1038/s41598-018-20978-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Low-cost Mass Production Process of Superrepellent Surface using Doubly Reentrant Structure Pillar Array2017

    • Author(s)
      中西完貴、簔城森幸、尹棟鉉、関口哲志、庄子習一
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 137 Issue: 6 Pages: 165-168

    • DOI

      10.1541/ieejsmas.137.165

    • NAID

      130005687853

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A High-Resolution Passive Droplet-Phase Sample Sorter Using Multi-Stage Droplet Transfer2017

    • Author(s)
      D.H. Yoon, Z, Xie, D. Tanaka, T-Sekiguchi, S. Shoji
    • Journal Title

      RSC Advances

      Volume: 58 Issue: 58 Pages: 36750-36754

    • DOI

      10.1039/c7ra05556k

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthesis of an Azo-Mn(II) Complex with Mild pH Control Using a Microfluidic Device2017

    • Author(s)
      D. Tanaka, S. Sawai, D.H. Yoon, T. Sekiguchi, T. Akitsu, S. Shoji
    • Journal Title

      RSC Advances

      Volume: 63 Issue: 63 Pages: 39576-39582

    • DOI

      10.1039/c7ra06089k

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an <i>ex vivo</i> Brain Lactic Acid Monitoring System2017

    • Author(s)
      S. Negishi, S. Kamada, R. Kajiwara, Y. Kaneko, D.H. Yoon, T. Sekiguchi, S. Shoji, H. Kudo
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 137 Issue: 1 Pages: 38-43

    • DOI

      10.1541/ieejsmas.137.38

    • NAID

      130005188402

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microfluidic Stamping on Sheath Flow2016

    • Author(s)
      2.D.H. Yoon, D. Tanaka, T. Sekiguchi, S. Shoji
    • Journal Title

      Small

      Volume: Vol. 12 Issue: 24 Pages: 32243228-32243228

    • DOI

      10.1002/smll.201600552

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column2016

    • Author(s)
      Y. Song, K. Takatsuki, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
    • Journal Title

      CHROMATOGRAPHY

      Volume: 37 Issue: 3 Pages: 111-116

    • DOI

      10.15583/jpchrom.2016.008

    • NAID

      130005277408

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array2016

    • Author(s)
      M. Isokawa, K. Takatsuki, Y.T. Song, K. Shih, K. Nakanishi, Z. Xie, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji,
    • Journal Title

      Analytical Chemistry

      Volume: 88 Issue: 12 Pages: 64856491-64856491

    • DOI

      10.1021/acs.analchem.6b01201

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Application of I-Structure Though-Glass Interconnect Filled with Submicron Gold Particles to a Hermetic Sealing Device2016

    • Author(s)
      K. Nomura, A. Okada, S. Shoji, T. Ogashiwa, J. Mizuno
    • Journal Title

      Journal of Micromechanics and Microengineering

      Volume: 26 Issue: 10 Pages: 17-17

    • DOI

      10.1088/0960-1317/26/10/105018

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Oil-In-Water Droplet Formation in Hydrophobic PDMS Device Using Three-Dimensional Protruded Taper Channel2019

    • Author(s)
      D.H. Yoon, T. Sekiguchi, S. Shoji
    • Organizer
      CIAiS (Research and Education Consortium for Innovation of Advanced Integrated Science) International Symposium 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ液滴を用いた効率的なアゾ化合物の合成2019

    • Author(s)
      澤井俊介、田中大器、尹棟鉉、関口哲志、秋津貴城、庄子習一
    • Organizer
      日本化学会 第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fundamental Research for Portable On-Demand Fluorescence Detection System2018

    • Author(s)
      A. Otsuka, H. Kuwae, K. Sakamoto, M. Suzuki, S. Shoji, J. Mizuno
    • Organizer
      2018 Japan-Taiwan Workshop on Electronic Interconnection II
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High Efficiency Synthesis of Br Addition Ligand by Microfluidic Device2018

    • Author(s)
      D. Tanaka, W. Kawakubo, K. Takahashi, D.H. Yoon,T. Sekiguchi, T. Akitsu, S. Shoji
    • Organizer
      43rd International Conference on Coordination Chemistry (ICCC 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Long Luminant Tissues in Three-Dimensional Gel Scaffolds with Oriented Cellulose Nanofibers2018

    • Author(s)
      T. Tanaka, K. Imato, K. Yoneda, D.H. Yoon, T. Sekiguchi, S. Shoji, N. Takeda
    • Organizer
      5th Tissue Engineering and Regenerative Medicine International Society World Congress 2018 (5th TERMIS World Congress 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Long Myotube Bundles in Three-Dimensional Gel Scaffolds with Oriented Cellulose Nanofibers2018

    • Author(s)
      K. Imato, K. Yoneda, T. Tanaka, D.H. Yoon, T. Sekiguchi, S. Shoji, N. Takeda
    • Organizer
      5th Tissue Engineering and Regenerative Medicine International Society World Congress 2018 (5th TERMIS World Congress 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Oil-In-Water Droplet Formation in Hydrophobic PDMS Device Using Three-Dimensional Protruded Taper Channel2018

    • Author(s)
      C. Tang, D.H. Yoon, T-Sekiguchi, S, Shoji
    • Organizer
      The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Measurement of the Cell Migration and Cytokine Secretion Activity with Real-Time Imaging System2018

    • Author(s)
      Y. Tanaka, N. Suzuki, K. Moro, J. Mizuno, S. Shoji, S. Uemura, Y. Shirasaki
    • Organizer
      29th 2018 International Symposium on Micro-NanoMechatronics and Human Science (MHS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 粘度の違いを利用したマイクロドロップレットソーティングデバイスの開発2018

    • Author(s)
      田中大器、菅谷拓央、川久保渉、尹棟鉉、関口哲志、庄子習一
    • Organizer
      第35回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] マイクロ流体デバイスと電界紡糸法によるマルチコンポーネントファイバーの作製2018

    • Author(s)
      菅谷拓央、深野愼介、尹棟鉉、関口哲志、庄子習一
    • Organizer
      第35回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Extremely Efficient and Non-Hazardous Bromo Group Addition Reaction Using Simple Microfluidic Devices2017

    • Author(s)
      W. Kawakubo, D. Tanaka, D.H. Yoon, T. Sekiguchi, K. Takahashi, T. Akitsu, S. Shoji,
    • Organizer
      The 3rd Conference on Microfluidic Handling Systems (MFHS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Crystallization of Zinc(II) Complex Containing Lysozyme by Super Water Repellent Doubly Reentrant Structure Umbrella Pillar Array2017

    • Author(s)
      D. Tanaka, W. Kawakubo, D.H. Yoon, T. Sekiguchi, T. Akitsu, S. Shoji
    • Organizer
      The 3rd Conference on Microfluidic Handling Systems (MFHS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A Micromachined Liquid Chromatography Chip with a Pillar Array Mixer for Post-Column Derivatization in the Analysis of Neurotransmitters2017

    • Author(s)
      M. Isokawa, K. Nakanishi, T. Kanamori, H. Zhuang, H. Yamazaki, T. Sugaya, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
    • Organizer
      The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Promoting Proliferation and Elongation of Bone Marrow-Derived Stem Cells via Nano-Grain Deposited Periodic Nanostructures2017

    • Author(s)
      M. Shiozawa, Y. Akiba, K. Uoshima, K. Eguchi, H. Kuwae, W. Fu, S. Shoji, J. Mizuno
    • Organizer
      2017 MRS (Materials Research Society) Fall Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Highly Bendable Transparent Electrode Using Mesh Patterned Indium Tin Oxide for Flexible Electronic Devices2017

    • Author(s)
      K. Sakamoto, H. Kuwae, N. Kobayashi, A. Nobori, S. Shoji, J. Mizuno
    • Organizer
      The 12th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fifty-Second Analysis of Oxidative Stress Markers in Human Plasma Using Amide-Modified Liquid Chromatography Chip2016

    • Author(s)
      M. Isokawa, K. Nakanishi, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In Vitro Selection of Novel Peptide Agonists for Human Somatostatin Receptor Subtype-2 Using a Water-In-Oil Microdroplet Platform2016

    • Author(s)
      T. Sakurai, R. Iizuka, Y. Nakamura, J. Ishii, A. Kondo, A. Iguchi, D.H. Yoon, T. Sekiguchi, S. Shoji, T. Funatsu
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Complex Check-Like Cross-Sectional Flow Formation for Fiber-Shaped Materials2016

    • Author(s)
      K. Kobayashi, D.H. Yoon, T. Sekiguchi, S. Shoji
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication Process of Fluidic Devices for Producing Fine Droplets2016

    • Author(s)
      Y. Nozaki, T. Kanai, A. Matsuo, D. Tanaka, I. Yuito, T. Takeuchi, T. Sekiguchi, S. Shoji
    • Organizer
      IEEE NANO 2016
    • Place of Presentation
      Sendai
    • Year and Date
      2016-08-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Low Temperature Bonding Between Polyether Ether Ketone (PEEK) and Pt Through Vapor Assisted VUV Surface Modification2016

    • Author(s)
      W. Fu, S. Shoji, J. Mizuno, A. Shigetou
    • Organizer
      2016 IEEE 66th Electronic Components and Technology Conference
    • Place of Presentation
      Las Vegas, USA
    • Year and Date
      2016-05-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] ピラーアレイミキサー及びこれを使用した試料処理装置2018

    • Inventor(s)
      角田誠、船津高志、関口哲志、庄子習一
    • Industrial Property Rights Holder
      角田誠、船津高志、関口哲志、庄子習一
    • Industrial Property Rights Type
      特許
    • Patent Publication Number
      2018-122295
    • Filing Date
      2018
    • Acquisition Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] フレキシブル透明電極及び有機エレクトロルミネッセンス素子2017

    • Inventor(s)
      水野潤、庄子習一、坂本暁祐、桑江博之
    • Industrial Property Rights Holder
      水野潤、庄子習一、坂本暁祐、桑江博之
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 積層構造体の製造方法2017

    • Inventor(s)
      庄子習一、水野潤、仁村将次、榎本智之、荻野浩司
    • Industrial Property Rights Holder
      庄子習一、水野潤、仁村将次、榎本智之、荻野浩司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Acquisition Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] ピラーアレイミキサー及びこれを使用した試料処理装置2017

    • Inventor(s)
      角田誠、磯川宗生、金森貴宏、船津高志、関口哲志、庄子習一
    • Industrial Property Rights Holder
      角田誠、磯川宗生、金森貴宏、船津高志、関口哲志、庄子習一
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-018648
    • Filing Date
      2017-02-03
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 接合方法2016

    • Inventor(s)
      重藤暁津、水野潤、庄子習一
    • Industrial Property Rights Holder
      重藤暁津、水野潤、庄子習一
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016
    • Acquisition Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 微細凹凸構造を表面に有する物品およびその製造方法2016

    • Inventor(s)
      高山公介、庄子習一、水野潤
    • Industrial Property Rights Holder
      高山公介、庄子習一、水野潤
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
    • Overseas

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Published: 2016-04-21   Modified: 2020-03-30  

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