Elucidation and application development of the fruiting body generating compounds of higher fungi

• Project/Area Number: 16H02546
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Shizuoka University
• Principal Investigator: Kawagishi HIrokazu 静岡大学, グリーン科学技術研究所, 教授 (70183283)
• Co-Investigator(Kenkyū-buntansha): 菅 敏幸 静岡県立大学, 薬学部, 教授 (10221904) ; 鈴木 智大 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (10649601) ; 崔 宰熏 静岡大学, 農学部, 助教 (40731633) ; 平井 浩文 静岡大学, 農学部, 教授 (70322138)
• Research Collaborator: Kuwahara Shigefumi ; Nishikawa Toshio
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000); Fiscal Year 2018: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000); Fiscal Year 2017: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000); Fiscal Year 2016: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
• Keywords: 高等菌類 / 子実体発生物質 / 生活環 / 制御物質 / キノコ / 子実体形成 / 子実体誘導 / 子実体 / 発生 / 誘導物質

Outline of Final Research Achievements

We aimed to clarify fruiting body-forming compound(s) common to mushrooms from four issues.　1) The proof of the theory that one of the fruiting body-forming compound(s) is steroids: Endogenous existence of strophasterols and chaxines in many mushrooms have been proved.　2) Search for fruiting body-forming compound(s) from fruiting liquid: A fruiting body-forming compound was found as a novel substance from fruiting liquid of Yamabushitake (Hericium erinaceus).　3) Demonstration of fairy chemicals as fruiting body-forming compound(s) in mushrooms: Endogenous existence of fairy chemicals in mushrooms was found, and they stimulated the growth of Matsutake (Tricholoma matsutake).　4) Search for life-cycle regulating molecules from spores: New substances were obtained from the spores of Yamabushitake.

Academic Significance and Societal Importance of the Research Achievements

４つの課題は全て研究代表者の研究成果，発想，仮説に基づいており，独創性は際立っている。キノコ共通の子実体発生物質（発茸ホルモン候補分子）を明らかにできれば，天然物化学，基礎生物学等における学術的成果は極めて大きく（NatureやScienceにも掲載可能），加えて，これまで不可能であったトリュフやマツタケの人工栽培への道を開き，産業，社会に与えるインパクトも極めて大きい。

Research Products

• [Journal Article] The complete mitochondrial genome sequence of the edible mushroom Stropharia rugosoannulata (Strophariaceae, Basidiomycota) (2019)
  • Author(s): Suzuki Tomohiro、Ono Akiko、Choi Jae-Hoon、Wu Jing、Kawagishi Hirokazu、Dohra Hideo
  • Journal Title: Mitochondrial DNA Part B Volume: 4 Issue: 1 Pages: 570-572
  • DOI: 10.1080/23802359.2018.1558120
  • NAID: 120006603032
  • Peer Reviewed / Open Access
• [Journal Article] Erinachromanes A and B and Erinaphenol A from the Culture Broth of Hericium erinaceus (2019)
  • Author(s): Wu Jing、Uchida Kazuki、Ridwan Arif Yanuar、Kondo Mitsuru、Choi Jae-Hoon、Hirai Hirofumi、Kawagishi Hirokazu
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 67 Issue: 11 Pages: 3134-3139
  • DOI: 10.1021/acs.jafc.8b06050
  • Peer Reviewed
• [Journal Article] Genome sequence analysis of the fairy ring-forming fungus Lepista sordida and gene candidates for interaction with plants (2019)
  • Author(s): Takano Tomoyuki、Yamamoto Naoki、Suzuki Tomohiro、Dohra Hideo、Choi Jae-Hoon、Terashima Yurika、Yokoyama Koji、Kawagishi Hirokazu、Yano Kentaro
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 5888-5888
  • DOI: 10.1038/s41598-019-42231-9
  • NAID: 120006825988
  • Peer Reviewed / Open Access
• [Journal Article] Bioactive compounds from the edible mushroom Cortinarius caperatus (2018)
  • Author(s): Ridwan, Y. A., Wu, J., Choi, J-H., Hirai, H., and Kawagishi, H.
  • Journal Title: Mycoscience Volume: 59 Issue: 2 Pages: 172-175
  • DOI: 10.1016/j.myc.2017.08.015
  • NAID: 120006533592
  • Peer Reviewed
• [Journal Article] Plant growth regulators from the fruiting bodies of Tricholoma flavovirens (2017)
  • Author(s): Qiu, W., Kobori, H., Wu, J., Choi－H., Hira, H., and Kawagishi, H.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 81 Issue: 3 Pages: 441-444
  • DOI: 10.1080/09168451.2016.1249453
  • NAID: 120006380813
  • Peer Reviewed
• [Journal Article] Cytotoxic compounds against cancer cells from Bombyx mori inoculated with Cordyceps militaris (2017)
  • Author(s): 1.Qiu, W., Wu, J., Choi, J.-H., Hira, H., Nishida, H., and Kawagishi, H.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 印刷中 Issue: 6 Pages: 1224-1226
  • DOI: 10.1080/09168451.2017.1289075
  • NAID: 120006395368
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plant growth regulators from the edible mushroom Leccinum extremiorientale (2017)
  • Author(s): Ito, A., Wu, J., Ozawa, N., Choi, J-H., Hirai, H., and Kawagishi, H.
  • Journal Title: Mycoscience Volume: 58 Issue: 6 Pages: 383-386
  • DOI: 10.1016/j.myc.2017.04.008
  • NAID: 120006492731
  • Peer Reviewed
• [Journal Article] Plant growth inhibitors from the culture broth of fairy ring-forming fungus Lepista sordida (2017)
  • Author(s): Ito, A., Choi, J-H., Wu, J., Tanaka, H., Hirai, H., and Kawagishi, H.
  • Journal Title: Mycoscience Volume: 58 Issue: 6 Pages: 387-390
  • DOI: 10.1016/j.myc.2017.05.001
  • NAID: 120006492725
  • Peer Reviewed
• [Journal Article] High-quality draft genome sequence of Burkholderia contaminans CH-1, a Gram-negative bacterium that metabolizes 2-azahypoxanthine, a plant growth-regulating compound (2017)
  • Author(s): Choi, J-H., Moriuchia, R., Sugiurac, H., Kawagishi, H, and Dohra, H.
  • Journal Title: Genome Announc. Volume: 5 Issue: 41
  • DOI: 10.1128/genomea.01148-17
  • NAID: 120006376296
  • Peer Reviewed / Open Access
• [Presentation] Chaxine類とその類縁体の合成研究 (2019)
  • Author(s): 二木美咲, 平田裕嗣，中崎敦夫, 河岸 洋和，西川俊夫
  • Organizer: 日本農芸化学会2019年度大会
• [Presentation] ヤマブシタケ（Hericium erinaceus）の生活環各段階における生物活性物質の網羅的探索 (2019)
  • Author(s): 呉静，石岡逹郎，崔宰熏，平井浩文，河岸洋和
  • Organizer: 日本農芸化学会2019年度大会
• [Presentation] Chaxine類とその類縁体の合成研究 (2018)
  • Author(s): 二木美咲, 平田裕嗣，中崎敦夫, 河岸 洋和，西川俊夫
  • Organizer: 日本農芸化学会 中部支部第183回 例会
• [Presentation] ヤマブシタケ（Hericium erinaceus）の生活環各段階における生物活性物質の網羅的探索 (2018)
  • Author(s): 呉静，内田和輝，石岡達朗，崔宰熏，平井浩文，河岸洋和
  • Organizer: 第60回天然有機化合物討論会
• [Presentation] ヤマブシタケ(Hericium erinaceus)培養ろ液由来の生理活性物質の探索 (2017)
  • Author(s): 内田和輝、呉静、崔宰熏、徳山真治、西田浩志、平井浩文、河岸洋和
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 京都女子大学（京都府京都市）
  • Year and Date: 2017-03-19
• [Presentation] フェアリー化合物によるキノコ菌糸体の成長制御に関する研究 (2017)
  • Author(s): 呉静、内田和輝、崔宰熏、平井浩文、菅敏幸、河岸洋和
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 京都女子大学（京都府京都市）
  • Year and Date: 2017-03-19