Deciphering the structural diversification mechanism by terpene synthase
| Project/Area Number |
16H03277
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Minami Atsushi 北海道大学, 理学研究院, 准教授 (40507191)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Keywords | 天然物 / セスタテルペン環化酵素 / 分子系統樹解析 / 生合成 / 酵素 / 天然物化学 |
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| Outline of Final Research Achievements |
Sesterterpene synthase identified from fungi are a class-I terpene synthase, which catalyzes the formation of macrocyclic rings fused with a cyclopentane moiety. The key cyclization mechanism has been extensively studied through heterologous expression, mutational analysis, and DFT calculations. The difference between cyclization mechanisms is consistent with phylogenetic analysis of those sesterterpene synthases, suggesting that phylogenetic analysis provides a useful guideline for the prediction of cyclization products. The generality of this guideline was demonstrated by functional analysis of fungal sesquiterpene synthases.
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| Academic Significance and Societal Importance of the Research Achievements |
テルペン環化酵素によって構築される天然有機化合物の中には、医薬品や香料などとして利用されているものが多い。抗マラリア剤として利用されているアルテミシニンが代表的な例である。本研究により、分子系統樹解析を用いて標的遺伝子を選択することで構造類縁体の網羅的かつ集中的な生産が可能であることが明らかになったため、将来的には、指数関数的に増加する遺伝子情報の中から新たな医薬品シードとなる環化体を与える酵素遺伝子を論理的に探索できるようになると考えている。
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Focused genome mining of structurally related sesterterpenes: enzymatic formation of enantiomeric and diastereomeric products2017
Author(s)
Narita, K., Sato, H., Minami, A., Kudo, K., Gao, L., Liu, C., Ozaki, T., Kodama, M.., Lei, X., Taniguchi, T., Monde, K., Yamazaki, M., Uchiyama, M., Oikawa, H.
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Journal Title
Organic letters
Volume: 19
Issue: 24
Pages: 6696-6699
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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