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Search for anti-leukemic drugs targeting the cancer-related transcription factor Runx1/CBFbeta

Research Project

Project/Area Number 16H03293
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical biology
Research InstitutionYokohama City University

Principal Investigator

Ogata Kazuhiro  横浜市立大学, 医学研究科, 教授 (90260330)

Co-Investigator(Kenkyū-buntansha) 浜田 恵輔  横浜市立大学, 医学部, 助教 (00344052)
仙石 徹  横浜市立大学, 医学部, 講師 (60576312)
椎名 政昭  横浜市立大学, 医学部, 助教 (30347299)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords転写因子 / 転写制御 / がん / 創薬 / 癌 / 医薬品探索 / 分子標的薬
Outline of Final Research Achievements

The molecule targeted therapy has been shown to be effective against various cancers. One of the potential targets is a transcription factor because its mutation is known to be frequently involved in cancer development. However, few drugs targeting transcription factors have been available. We have aimed to develop anti-leukemic drugs targeting a transcription factor, Runx1. Runx1 is a master regulator of the blood cell development, and various mutations of Runx1 gene have frequently been found in acute myelogenous leukemia (AML) patients.
We have searched for compounds that could destabilize the regulatory region of Runx1-DNA binding based on the molecular structure, and verified that some compounds have an ability to bind to Runx1 and inhibit DNA binding of Runx1. Furthermore, we have shown that these compounds could inhibit the transcriptional activation ability of Runx1 and decrease the cell-survival rate for the Kasumi-1 AML cells with the Runx1 mutation.

Academic Significance and Societal Importance of the Research Achievements

変異型の転写因子は、ドミナント・ネガティブな様式で転写制御異常を誘起し、細胞の分化異常やがん化の原因となり得る。そのため、変異分子を含んだ異常な複合体の形成要因となる分子間相互作用を標的とした阻害薬は有望な治療薬候補となる。我々は急性骨髄性白血病の発症に関わる主要な転写因子の一つであるRunx1のDNA結合活性における制御部位を分子構造解析と機能解析の結果から明らかにし、その領域を介してアロステリックな様式で作用し得るDNA結合阻害薬を複数見出した。今後、これら阻害薬の構造の最適化を目指す。これまでに転写因子を標的とした阻害薬の報告例は極めて少ないため、大きな波及効果を生むことを期待している。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (34 results)

All 2019 2018 2017 Other

All Journal Article (22 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 22 results,  Acknowledgement Compliant: 5 results,  Open Access: 7 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Germline-Derived Gain-of-Function Variants of Gsα-Coding GNAS Gene Identified in Nephrogenic Syndrome of Inappropriate Antidiuresis2019

    • Author(s)
      Miyado Mami、Fukami Maki、Takada Shuji、Terao Miho、Nakabayashi Kazuhiko、Hata Kenichiro、Matsubara Yoichi、Tanaka Yoko、Sasaki Goro、Nagasaki Keisuke、Shiina Masaaki、Ogata Kazuhiro、Masunaga Youhei、Saitsu Hirotomo、Ogata Tsutomu
    • Journal Title

      Journal of the American Society of Nephrology

      Volume: 30 Issue: 5 Pages: 877-889

    • DOI

      10.1681/asn.2018121268

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphorylation of an intrinsically disordered region of Ets1 shifts a multi-modal interaction ensemble to an out-inhibitory state.2018

    • Author(s)
      Kota Kasahara, Masaaki Shiina, Junichi Higo, Kazuhiro Ogata, Haruki Nakamura.
    • Journal Title

      Nucleic Acids Research

      Volume: 46 Issue: 5 Pages: 2243-2251

    • DOI

      10.1093/nar/gkx1297

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo hotspot variants in CYFIP2 cause early-onset epileptic encephalopathy2018

    • Author(s)
      Nakashima Mitsuko、Kato Mitsuhiro、Aoto Kazushi、Shiina Masaaki、Belal Hazrat、Mukaida Souichi、Kumada Satoko、Sato Atsushi、Zerem Ayelet、Lerman-Sagie Tally、Lev Dorit、Leong Huey Yin、Tsurusaki Yoshinori、Mizuguchi Takeshi、Miyatake Satoko、Miyake Noriko、Ogata Kazuhiro、Saitsu Hirotomo、Matsumoto Naomichi
    • Journal Title

      Annals of Neurology

      Volume: 83 Issue: 4 Pages: 794-806

    • DOI

      10.1002/ana.25208

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Loss-of-function and gain-of-function mutations in PPP3CA cause two distinct disorders2018

    • Author(s)
      Mizuguchi Takeshi、Kurahashi Hirokazu、et al.
    • Journal Title

      Human Molecular Genetics

      Volume: 27 Issue: 8 Pages: 1421-1433

    • DOI

      10.1093/hmg/ddy052

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A homozygous NOP14 variant is likely to cause recurrent pregnancy loss2018

    • Author(s)
      Suzuki Toshifumi、Behnam Mahdiyeh、Ronasian Firooze、Salehi Mansoor、Shiina Masaaki、Koshimizu Eriko、Fujita Atsushi、Sekiguchi Futoshi、Miyatake Satoko、Mizuguchi Takeshi、Nakashima Mitsuko、Ogata Kazuhiro、Takeda Satoru、Matsumoto Naomichi、Miyake Noriko
    • Journal Title

      Journal of Human Genetics

      Volume: 63 Issue: 4 Pages: 425-430

    • DOI

      10.1038/s10038-018-0410-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Heterozygous Mutations in OAS1 Cause Infantile-Onset Pulmonary Alveolar Proteinosis with Hypogammaglobulinemia.2018

    • Author(s)
      Cho K, Yamada M, Agematsu K, Kanegane H, Miyake N, Ueki M, Akimoto T, Kobayashi N, Ikemoto S, Tanino M, Fujita A, Hayasaka I, Miyamoto S, Tanaka-Kubota M, Nakata K, Shiina M, Ogata K, Minakami H, Matsumoto N, Ariga T.
    • Journal Title

      Am J Hum Genet

      Volume: 102 Issue: 3 Pages: 480-486

    • DOI

      10.1016/j.ajhg.2018.01.019

    • NAID

      120006502505

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo variants in CAMK2A and CAMK2B cause neurodevelopmental disorders2018

    • Author(s)
      Akita Tenpei、Aoto Kazushi、Kato Mitsuhiro、Shiina Masaaki、Mutoh Hiroki、Nakashima Mitsuko、Kuki Ichiro、Okazaki Shin、Magara Shinichi、Shiihara Takashi、Yokochi Kenji、Aiba Kaori、Tohyama Jun、Ohba Chihiro、Miyatake Satoko、Miyake Noriko、Ogata Kazuhiro、Fukuda Atsuo、Matsumoto Naomichi、Saitsu Hirotomo
    • Journal Title

      Annals of Clinical and Translational Neurology

      Volume: 5 Issue: 3 Pages: 280-296

    • DOI

      10.1002/acn3.528

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy2018

    • Author(s)
      Fassio Anna、Esposito Alessandro、Kato Mitsuhiro、Saitsu Hirotomo、et al.
    • Journal Title

      Brain

      Volume: 141 Issue: 6 Pages: 1703-1718

    • DOI

      10.1093/brain/awy092

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A novel CYCS mutation in the α‐helix of the CYCS C‐terminal domain causes non‐syndromic thrombocytopenia2018

    • Author(s)
      Uchiyama Yuri、Yanagisawa Kunio、Kunishima Shinji、Shiina Masaaki、Ogawa Yoshiyuki、Nakashima Mitsuko、Hirato Junko、Imagawa Eri、Fujita Atsushi、Hamanaka Kohei、Miyatake Satoko、Mitsuhashi Satomi、Takata Atsushi、Miyake Noriko、Ogata Kazuhiro、Handa Hiroshi、Matsumoto Naomichi、Mizuguchi Takeshi
    • Journal Title

      Clinical Genetics

      Volume: 94 Issue: 6 Pages: 548-553

    • DOI

      10.1111/cge.13423

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biallelic COLGALT1 variants are associated with cerebral small vessel disease2018

    • Author(s)
      Miyatake Satoko、Schneeberger Sacha、Koyama Norihisa、Yokochi Kenji、Ohmura Kayo、、Hennet Thierry、Matsumoto Naomichi、et al
    • Journal Title

      Annals of Neurology

      Volume: 84 Issue: 6 Pages: 843-853

    • DOI

      10.1002/ana.25367

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Homozygous splicing mutation in NUP133 causes Galloway-Mowat syndrome2018

    • Author(s)
      Fujita Atsushi、Tsukaguchi Hiroyasu、Koshimizu Eriko、Nakazato Hitoshi、Itoh Kyoko、Kuraoka Shohei、Komohara Yoshihiro、Shiina Masaaki、Nakamura Shohei、Kitajima Mika、Tsurusaki Yoshinori、Miyatake Satoko、Ogata Kazuhiro、Iijima Kazumoto、Matsumoto Naomichi、Miyake Noriko
    • Journal Title

      Annals of Neurology

      Volume: 84 Issue: 6 Pages: 814-828

    • DOI

      10.1002/ana.25370

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GRIN2D variants in three cases of developmental and epileptic encephalopathy2018

    • Author(s)
      Tsuchida Naomi、Hamada Keisuke、Shiina Masaaki、Kato Mitsuhiro、Kobayashi Yu、Tohyama Jun、Kimura Kazue、Hoshino Kyoko、Ganesan Vigneswari、Teik Keng W.、Nakashima Mitsuko、Mitsuhashi Satomi、Mizuguchi Takeshi、Takata Atsushi、Miyake Noriko、Saitsu Hirotomo、Ogata Kazuhiro、Miyatake Satoko、Matsumoto Naomichi
    • Journal Title

      Clinical Genetics

      Volume: 94 Issue: 6 Pages: 538-547

    • DOI

      10.1111/cge.13454

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Thioether Macrocyclic Peptides Selected against TET1 Compact Catalytic Domain Inhibit TET1 Catalytic Activity2018

    • Author(s)
      Nishio Kosuke、Belle Roman、Katoh Takayuki、Kawamura Akane、Sengoku Toru、Hanada Kazuharu、Ohsawa Noboru、Shirouzu Mikako、Yokoyama Shigeyuki、Suga Hiroaki
    • Journal Title

      ChemBioChem

      Volume: 19 Issue: 9 Pages: 979-985

    • DOI

      10.1002/cbic.201800047

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural basis of protein arginine rhamnosylation by glycosyltransferase EarP2018

    • Author(s)
      Sengoku Toru、Suzuki Takehiro、Dohmae Naoshi、Watanabe Chiduru、Honma Teruki、Hikida Yasushi、Yamaguchi Yoshiki、Takahashi Hideyuki、Yokoyama Shigeyuki、Yanagisawa Tatsuo
    • Journal Title

      Nature Chemical Biology

      Volume: 14 Issue: 4 Pages: 368-374

    • DOI

      10.1038/s41589-018-0002-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel GFI1B mutation at the first zinc finger domain causes congenital macrothrombocytopenia2017

    • Author(s)
      Uchiyama Y, Ogawa Y, Kunishima S, Shiina M, Nakashima M, Yanagisawa K, Yokohama A, Imagawa E, Miyatake S, Mizuguchi T, Takata A, Miyake N, Ogata K, Handa H, Matsumoto N
    • Journal Title

      British Journal of Haematology

      Volume: 印刷中 Issue: 6 Pages: 843-847

    • DOI

      10.1111/bjh.14710

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A novel missense mutation affecting the same amino acid as the recurrent PACS1 mutation in Schuurs-Hoeijmakers syndrome2017

    • Author(s)
      Miyake N, Ozasa S, Mabe H, Kimura S, Shiina M, Imagawa E, Miyatake S, Nakashima M, Mizuguchi T, Takata A, Ogata K, Matsumoto N
    • Journal Title

      Clinical Genetics

      Volume: 93 Issue: 4 Pages: 929-930

    • DOI

      10.1111/cge.13105

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A novel mutation in SLC1A3 causes episodic ataxia2017

    • Author(s)
      Iwama Kazuhiro、Iwata Aya、Shiina Masaaki、Mitsuhashi Satomi、Miyatake Satoko、Takata Atsushi、Miyake Noriko、Ogata Kazuhiro、Ito Shuichi、Mizuguchi Takeshi、Matsumoto Naomichi
    • Journal Title

      Journal of Human Genetics

      Volume: 63 Issue: 2 Pages: 207-211

    • DOI

      10.1038/s10038-017-0365-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biallelic mutations in MYPN, encoding Myopalladin, are associated with childhood-onset, slowly progressive nemaline myopathy2017

    • Author(s)
      Miyatake S, Mitsuhashi S, Hayashi YK, Purevjav E, Nishikawa A, Koshimizu E, Suzuki M, Yatabe K, Tanaka Y, Ogata K, Kuru S, Shiina M, Tsurusaki Y, Nakashima M, Mizuguchi T, Miyake N, Saitsu H, Ogata K, Kawai M, Towbin J, Nonaka I, Nishino I, Matsumoto N
    • Journal Title

      American Journal of Human Genetics

      Volume: 100 Issue: 1 Pages: 169-178

    • DOI

      10.1016/j.ajhg.2016.11.017

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The first report of Japanese patients with asparagine synthetase deficiency2017

    • Author(s)
      Yamamoto T, Endo W, Ohnishi H, Kubota K, Kawamoto N, Inui T, Imamura A, Takanashi JI, Shiina M, Saitsu H, Ogata K, Matsumoto N, Haginoya K, Fukao T
    • Journal Title

      Brain Development

      Volume: 39 Issue: 3 Pages: 236-242

    • DOI

      10.1016/j.braindev.2016.09.010

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PARS2 and NARS2 mutations in infantile-onset neurodegenerative disorder2017

    • Author(s)
      Mizuguchi T, Nakashima M, Kato M, Yamada K, Okanishi T, Ekhilevitch N, Mandel H, Eran A, Toyono M, Sawaishi Y, Motoi H, Shiina M, Ogata K, Miyatake S, Miyake N, Saitsu H, Matsumoto N
    • Journal Title

      Journal of Human Genetics

      Volume: 62 Issue: 5 Pages: 525-529

    • DOI

      10.1038/jhg.2016.163

    • NAID

      40021210259

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular mechanisms of cooperative binding of transcription factors Runx1-CBFβ-Ets1 on the TCRα gene enhancer2017

    • Author(s)
      Kasahara K, Shiina M, Fukuda I, Ogata K, Nakamura H
    • Journal Title

      PloS One

      Volume: 12 Issue: 2 Pages: e0172654-e0172654

    • DOI

      10.1371/journal.pone.0172654

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Biallelic mutations in the 3' exonuclease TOE1 cause pontocerebellar hypoplasia and uncover a role in snRNA processing2017

    • Author(s)
      Lardelli RM, Schaffer AE, Eggens VR, ... Matsumoto N, Muramatsu K, Saitsu H, Shiina M, Ogata K, Foulds N, Dobyns WB, Chi NC, Traver D, Spaccini L, Bova SM, Gabriel SB, Gunel M, Valente EM, Nassogne MC, Bennett EJ, Yeo GW, Baas F, Lykke-Andersen J, Gleeson JG
    • Journal Title

      Nature Genetics

      Volume: 49 Issue: 3 Pages: 457-464

    • DOI

      10.1038/ng.3762

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] メチル基を数える: ヒストンH4K20のメチル化状態を特異的に認識する抗体の構造2018

    • Author(s)
      仙石 徹,渡邉千鶴,馬場しほ,本間光貴,緒方一博,木村 宏,横山茂之
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural basis for the recognition of the antioxidant response element by the Nrf2-MafG heterodimer2018

    • Author(s)
      Sengoku T, Shiina M, Uchiyama A, Baba S, Okada C, Sato K, Hamada K, Ogata K
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Search for anti-leukemic drugs targeting the transcription factor Runx1 by INTENDD2017

    • Author(s)
      Shiina M, Baba S, Uchiyama A, Okada C, Tanaka T, Ikeda K, Kawakita S, Matsuzaki T, Komatsu H, Hosoda M, Ogata K
    • Organizer
      The 137th Annual Meeting of the Pharmaceutical Society of Japan, 3rd International Symposium for Medicinal Sciences
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 転写因子Ets1の天然変性領域のリン酸化によるDNA結合制御機構の速度論的解析2017

    • Author(s)
      内山晃子, 椎名政昭, 浜田恵輔, 馬場しほ, 岡田千佳子, 鈴木香絵, 佐藤 光, 緒方一博
    • Organizer
      新学術領域研究「転写サイクル」冬の若手ワークショップ2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分子構造に基づく転写因子Nrf2の抗酸化剤応答配列認識機構の解析2017

    • Author(s)
      椎名政昭, 鈴木香絵, 浜田恵輔, 内山晃子, 岡田千佳子, 馬場しほ, 太田 力, 緒方一博
    • Organizer
      新学術領域研究「転写サイクル」冬の若手ワークショップ2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] パートナー転写因子存在下における Ets1のコンフォーメーション分布の変化と活性制御機構2017

    • Author(s)
      内山晃子,椎名政昭,浜田恵輔,馬場しほ,岡田千佳子,鈴木香絵,佐藤 光,緒方一博
    • Organizer
      2017年度生命科学系学会合同年次大会(第40回日本分子生物学会年会・第90回日本生化学会大会)
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] 転写因子 Nrf2による酸化ストレス応答配列 (ARE) の認識機構の解析2017

    • Author(s)
      椎名政昭,鈴木香絵,内山晃子,浜田恵輔,馬場しほ,岡田千佳子,太田 力,緒方一博
    • Organizer
      2017年度生命科学系学会合同年次大会(第40回日本分子生物学会年会・第90回日本生化学会大会)
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Search for anti-leukemic drugs targeting the transcription factor Runx1 by INTENDD.2017

    • Author(s)
      Shiina M, Baba S, Uchiyama A, Okada C, Tanaka T, Ikeda K, Kawakita S, Matsuzaki T, Komatsu H, Hosoda M, Ogata K
    • Organizer
      The 137th Annual Meeting of the Pharmaceutical Society of Japan, 3rd International Symposium for Medicinal Sciences
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] パートナー転写因子存在下におけるEts1のコンフォーメーション分布の変化と活性制御機構2017

    • Author(s)
      内山晃子, 椎名政昭, 浜田恵輔, 馬場しほ, 岡田千佳子, 鈴木香絵, 佐藤 光, 緒方一博
    • Organizer
      新学術領域研究「転写サイクル」冬の若手ワークショップ2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] 転写因子Ets1の天然変性領域のリン酸化によるDNA結合制御機構の速度論的解析2017

    • Author(s)
      椎名政昭, 笠原浩太, 肥後順一, 浜田恵輔, 馬場しほ, 内山晃子, 佐藤 光, 岡田千佳子, 中村春木, 緒方一博
    • Organizer
      新学術領域研究「転写サイクル」冬の若手ワークショップ2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] 分子構造に基づく転写因子Nrf2の抗酸化剤応答配列認識機構の解析2017

    • Author(s)
      椎名政昭, 鈴木香絵, 浜田恵輔, 内山晃子, 岡田千佳子, 馬場しほ, 太田 力, 緒方一博
    • Organizer
      新学術領域研究「転写サイクル」冬の若手ワークショップ2017
    • Related Report
      2016 Annual Research Report
  • [Remarks] 横浜市立大学大学院医学研究科生化学教室

    • URL

      http://www-user.yokohama-cu.ac.jp/~seika/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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