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Development of cell culture device utilising light response of ceramic semiconductor

Research Project

Project/Area Number 16H04521
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural/Functional materials
Research InstitutionKansai University

Principal Investigator

Ueda Masato  関西大学, 化学生命工学部, 教授 (40362660)

Co-Investigator(Kenkyū-buntansha) 池田 勝彦  関西大学, 化学生命工学部, 教授 (20184434)
松垣 あいら  大阪大学, 工学研究科, 助教 (10592529)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords生体材料 / セラミックス / 光化学反応
Outline of Final Research Achievements

Attachment/detachment of cells to/from substrates was tried to control without enzyme treatments by using photo-responsible ceramic films and light irradiation. TiO2 and SrTiO3 are n-type semiconductors with an energy band gap of 3.0-3.2 eV, which display photocatalytic reactions under ultraviolet (UV). Such films were prepared on SiO2 by a RF sputtering. Basically the number of cells monotonically increased with incubation periods under darkness. Previous UV irradiation promoted the cell adhesion. In contrast, the cells decreased under continuous UV irradiation. The cells were not exposed to UV in the vessels since it was completely absorbed by the ceramics layer. These results indicate that the adhesion/proliferation/detachment behaviours of cells can be controlled by the photocatalytic reactions of ceramic films and the irradiation patterns. Furthermore, two-dimensional patterning of attached cells by light irradiation could be performed in the vessels with simple structure.

Academic Significance and Societal Importance of the Research Achievements

紫外線(UV)に応答するTiO2とSrTiO3をSiO2基盤上に成膜したシンプルな構造を有する細胞培養器を開発し,光照射によって細胞接着の促進や抑制を制御できることを実験的に示した。なお,UV照射は培養器背面から行い,細胞はUVに曝されない構造とした。これは,細胞にダメージを与えてしまう従来の酵素処理を用いた細胞制御に代わる有効な手法である。さらに,細胞の2次元配置も可能となった。本技術を利用すれば,複数の細胞を任意に配置した細胞シートを作製することが可能となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (34 results)

All 2019 2018 2017 2016 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (28 results) (of which Int'l Joint Research: 13 results,  Invited: 4 results) Remarks (1 results)

  • [Journal Article] Evaluation of Microstructure by Precise Measurement of Resistivity in Pure Ti2018

    • Author(s)
      UEDA Masato、SAKAMOTO Takanori、IKEDA Masahiko
    • Journal Title

      Resources Processing

      Volume: 65 Issue: 3 Pages: 74-76

    • DOI

      10.4144/rpsj.65.74

    • NAID

      130007618811

    • ISSN
      1348-6012, 1349-9262
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flux growth of β-NaGaO2 single crystals2018

    • Author(s)
      Suzuki Issei、Kakinuma Ayako、Ueda Masato、Omata Takahisa
    • Journal Title

      Journal of Crystal Growth

      Volume: 504 Pages: 26-30

    • DOI

      10.1016/j.jcrysgro.2018.09.034

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of Cell Adhesion on Titanium Dioxide by Light Irradiation2018

    • Author(s)
      Ueda Masato、Yamaguchi Rika、Fujita Chika、Ikeda Masahiko
    • Journal Title

      Materials Science Forum

      Volume: 941 Pages: 2507-2512

    • DOI

      10.4028/www.scientific.net/msf.941.2507

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical Properties of Additively Manufactured Porous Titanium with Sub-Millimetre Structural Units2018

    • Author(s)
      Masato Ueda, Masahiko Ikeda, Shigeo Mori, Kenji Doi, Hisashi Kitagaki, Shuntaro Terauchi
    • Journal Title

      Mater. Trans.

      Volume: -

    • NAID

      130007695407

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of Porous Titanium-Polyglycolide-Calcium Carbonate Composite as Bone Replacement Material2018

    • Author(s)
      75.上田 正人, 池田 勝彦, 土井 研児, 森 重雄, 北垣 壽, 寺内 俊太郎, 関 あずさ
    • Journal Title

      KOBUNSHI RONBUNSHU

      Volume: 75 Issue: 1 Pages: 69-74

    • DOI

      10.1295/koron.2017-0056

    • NAID

      130006320112

    • ISSN
      0386-2186, 1881-5685
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] サブミリメートルの構造部位を有するポーラスチタンの機械的性質2019

    • Author(s)
      上田正人,足立まりあ,池田勝彦,森重雄,土井研児,北垣壽,寺内俊太郎
    • Organizer
      日本金属学会2019年春期(第164回)講演大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Detection of Lattice Defects in Pure Titanium by Resistivity Measurement2018

    • Author(s)
      M. Ueda, T. Sakamoto, M. Ikeda
    • Organizer
      The 16th Korea/Japan International Symposium on Recourses Recycling and Materials Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Preparation of SrTiO3 Thin Film for Cell Culture Vessel by RF Magnetron Sputtering2018

    • Author(s)
      S. Matsumura, M. Ueda, M. Ikeda
    • Organizer
      The 16th Korea/Japan International Symposium on Recourses Recycling and Materials Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 2D Patterning of Cells by Light Irradiation2018

    • Author(s)
      M. Ueda
    • Organizer
      Finland-Japan Workshop: The next generation medical engineering in biomaterials, Joint with 3rd Bone and Biomaterials Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Control of Cell Adhesion on Titanium Dioxide by Light Irradiation2018

    • Author(s)
      M. Ueda, R. Yamaguchi, C. Fujita, M. Ikeda
    • Organizer
      THERMEC’2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Elastic-modulus enhancement during room-temperature aging in β-Ti alloys2018

    • Author(s)
      M. Tane, A. Umeda, K. Hagihara, M. Ueda, T. Nakano, T. Sekino, T. Ichitsubo
    • Organizer
      THERMEC’2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Low Cost Titanium-Manganese Shape Memory Alloys2018

    • Author(s)
      M. Ikeda, M. Ueda
    • Organizer
      THERMEC’2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Influence of Oxygen on Phase Constitution and Heat Treatment in Ti-V Alloys2018

    • Author(s)
      H. Nagasawa, M. Ikeda, M. Ueda
    • Organizer
      THERMEC’2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ラット脛骨に埋植したポーラスチタン-ポリグリコール酸-炭酸カルシウム複合体周囲の骨組織2018

    • Author(s)
      上田正人,池田勝彦,関あずさ
    • Organizer
      第38回日本骨形態計測学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] セラミック半導体を利用した光応答型細胞培養器の試作2018

    • Author(s)
      永澤 瞳,山口莉嘉,上田正人,池田勝彦
    • Organizer
      日本バイオマテリアル学会関西ブロック 第13回若手研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] RFスパッタリングによるSrTiO3の成膜と光応答性2018

    • Author(s)
      松村漱,山口莉嘉,上田正人,池田勝彦
    • Organizer
      日本金属学会2018年秋期(第163回)講演大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Ti-20mass%V-酸素合金の熱処理挙動に及ぼす酸素添加量の影響2018

    • Author(s)
      永澤瞳,池田勝彦,上田正人
    • Organizer
      日本金属学会2018年秋期(第163回)講演大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光応答型細胞培養器における局所的な電位制御2018

    • Author(s)
      上田正人,山口莉嘉,藤田智香,池田勝彦
    • Organizer
      日本金属学会2018年春期(第162回)講演大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光応答細胞培養器における光応答性の鋭敏化2017

    • Author(s)
      上田正人,藤田智香,池田勝彦
    • Organizer
      日本金属学会
    • Place of Presentation
      首都大学東京 南大沢キャンパス
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 背面照射型のTi/TiO2/水溶液/SiO2光応答細胞培養器の試作2017

    • Author(s)
      藤田智香,上田正人,池田勝彦
    • Organizer
      日本金属学会
    • Place of Presentation
      首都大学東京 南大沢キャンパス
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fabrication of Photo-Responsible Cell Culture Vessels using Titanium Dioxide2017

    • Author(s)
      C. Fujita, M. Ueda, M. Ikeda
    • Organizer
      The 15th Japan/Korea International Symposium on Recourses Recycling and Materials Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sensitization of Photo-Response in Cell Culture Vessels2017

    • Author(s)
      M. Ueda, C. Fujita, M. Ikeda
    • Organizer
      The 15th Japan/Korea International Symposium on Recourses Recycling and Materials Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Photo-Responsible Cell Culture Vessels using Titanium Oxide films2017

    • Author(s)
      M. Ueda, C. Fujita, M. Ikeda
    • Organizer
      2017 MRS Fall Meeting & Exhibit
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光応答細胞培養器における光応答性の鋭敏化2017

    • Author(s)
      上田正人,藤田智香,池田勝彦
    • Organizer
      日本金属学会2017年春期(第160回)講演大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 背面照射型のTi/TiO2/水溶液/SiO2光応答細胞培養器の試作2017

    • Author(s)
      藤田智香,上田正人,池田勝彦
    • Organizer
      日本金属学会2017年春期(第160回)講演大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 背面照射型TiO2/SiO2光応答細胞培養器の試作とその諸特性2017

    • Author(s)
      山口莉嘉,山本彩乃,藤田智香,上田正人,池田勝彦
    • Organizer
      日本バイオマテリアル学会関西ブロック 第12回若手研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 背面照射型Ti/TiO2/Hanks液/SiO2光応答細胞培養器の試作と光応答鋭敏化2017

    • Author(s)
      藤田智香,上田正人,池田勝彦
    • Organizer
      日本バイオマテリアル学会関西ブロック 第12回若手研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 背面照射型Ti/TiO2/Hanks液/SiO2光応答細胞培養器の試作2017

    • Author(s)
      藤田智香,上田正人,池田勝彦,松垣あいら,中野貴由
    • Organizer
      日本金属学会2017年秋期(第161回)講演大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸化チタン薄膜を利用した光応答型細胞培養器の試作2017

    • Author(s)
      上田正人,山口莉嘉,藤田智香,池田勝彦
    • Organizer
      第39回日本バイオマテリアル学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸化チタンの光化学反応を利用した背面照射型細胞培養器の試作2016

    • Author(s)
      上田正人,池田勝彦,藤田智香
    • Organizer
      日本バイオマテリアル学会シンポジウム2016
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-11-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fabrication of Photo-Responsible Cell Culture Vessels using TiO2 film2016

    • Author(s)
      Masato Ueda, Chika Fujita, Masahiko Ikeda, Aira Matsugaki, Takayoshi Nakano
    • Organizer
      PRICM9
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2016-08-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Utilisation of Photocatalytic Reaction of Titanium Dioxide in Biomedical Materials2016

    • Author(s)
      Masato Ueda, Masahiko Ikeda
    • Organizer
      The 14th Korea / Japan International Symposium on Resources Recycling and Materials Science
    • Place of Presentation
      Gyeong Won Jae Ambassador, Incheon, South Korea
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Utilisation of Titanium dioxide for Photo-responsive Cell Vessels2016

    • Author(s)
      Chika Fujita, Yasuka Yoshida, Masato Ueda, Masahiko Ikeda
    • Organizer
      The 14th Korea / Japan International Symposium on Resources Recycling and Materials Science
    • Place of Presentation
      Gyeong Won Jae Ambassador, Incheon, South Korea
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 関西大学 化学生命工学部 化学・物質工学科 環境材料研究室

    • URL

      http://www.chemmater.kansai-u.ac.jp/ecmate/

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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