Development of a new generation method of transgenic common marmoset, a potential resource for various researches

• Project/Area Number: 16H04650
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Niigata University
• Principal Investigator: Sakimura Kenji 新潟大学, 脳研究所, フェロー (40162325)
• Co-Investigator(Kenkyū-buntansha): 阿部 学 新潟大学, 脳研究所, 准教授 (10334674) ; 夏目 里恵 新潟大学, 脳研究所, 技術職員 (60467082) ; 中務 胞 新潟大学, 脳研究所, 助教 (60641579)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: マーモセット / 異種動物間キメラ / ES細胞 / 胚盤胞補完法 / ＥＳ細胞 / 異種間キメラ / 遺伝子編集 / 遺伝子改変 / コモンマーモセット / 臓器移植

Outline of Final Research Achievements

Creation of genetically modified models of primate marmosets is indispensable to understand human brain functions and diseases, but there have been economic and ethical barriers that have made it difficult to put into practice until very recently. In this study, to overcome these problems, we examined a method of obtaining marmoset fertilized eggs, which is both inexpensive and considerate to animal welfare. We transplanted ovarian tissues obtained from disused and dead individuals into the kidney capsule of nude mice, and with the addition of hormonal administration succeeded in obtaining eggs from the graft. Furthermore, we developed a new method of producing fertilized eggs which can grow to a morula stage, from the acquired eggs.

Academic Significance and Societal Importance of the Research Achievements

多くの研究者が多様な発想で遺伝子改変マーモセットを用いるには経済的・倫理的障壁があり、現実的に困難である。本研究により、マーモセット受精卵を安価かつ動物福祉的にも問題のない手法で取得することが可能になった。この成果は、遺伝子編集などの手法を用いて、疾患モデルを作出するためのブレークスルーになり、ヒト脳疾患理解に大きな貢献をするものである。

Research Products

• [Int'l Joint Research] Institute of Science and Technology(オーストリア)
• [Int'l Joint Research] DANDRITE, Aarhus University(デンマーク)
• [Journal Article] Autophosphorylation of F-actin binding domain of CaMKIIβ is required for fear learning (2019)
  • Author(s): Kim Karam、Suzuki Akio、Kojima Hiroto、Kawamura Meiko、Miya Ken、Abe Manabu、Yamada Ikuko、Furuse Tamio、Wakana Shigenaru、Sakimura Kenji、Hayashi Yasunori
  • Journal Title: Neurobiology of Learning and Memory Volume: 157 Pages: 86-95
  • DOI: 10.1016/j.nlm.2018.12.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas (2019)
  • Author(s): Shimizu Takeshi、Osanai Yasuyuki、Tanaka Kenji F.、Thai Truc Quynh、Abe Manabu、Natsume Rie、Sakimura Kenji、Ikenaka Kazuhiro
  • Journal Title: Journal of Neurochemistry Volume: 2019 Issue: 2 Pages: 158-172
  • DOI: 10.1111/jnc.14657
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Input-specific NMDAR-Dcpendent potentiation of dendritic GABAergic inhibition. (2018)
  • Author(s): Chiu CQ, Martenson JS, Yamazaki M, Natsume R, Sakimura K, Tomita S, Tavalin SJ, Higley MJ.
  • Journal Title: Ncuron. Volume: 97 Issue: 2 Pages: 368-377
  • DOI: 10.1016/j.neuron.2017.12.032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeting the SphK1/S1P/S1PR1 Axis That Links Obesity, Chronic Inflammation, and Breast Cancer Metastasis (2018)
  • Author(s): Nagahashi Masayuki、Yamada Akimitsu、Katsuta Eriko、Aoyagi Tomoyoshi、Huang Wei-Ching、Terracina Krista P.、Hait Nitai C.、Allegood Jeremy C.、Tsuchida Junko、Yuza Kizuki、Nakajima Masato、Abe Manabu、Sakimura Kenji、Milstien Sheldon、Wakai Toshifumi、Spiegel Sarah、Takabe Kazuaki
  • Journal Title: Cancer Research Volume: 78 Issue: 7 Pages: 1713-1725
  • DOI: 10.1158/0008-5472.can-17-1423
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum (2018)
  • Author(s): Uesaka Naofumi、Abe Manabu、Konno Kohtarou、Yamazaki Maya、Sakoori Kazuto、Watanabe Takaki、Kao Tzu-Huei、Mikuni Takayasu、Watanabe Masahiko、Sakimura Kenji、Kano Masanobu
  • Journal Title: Neuron Volume: 97 Issue: 4 Pages: 796-805
  • DOI: 10.1016/j.neuron.2018.01.018
  • Peer Reviewed / Open Access
• [Journal Article] Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum (2018)
  • Author(s): Nakayama Hisako、Abe Manabu、Morimoto Chie、Iida Tadatsune、Okabe Shigeo、Sakimura Kenji、Hashimoto Kouichi
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 2830-2830
  • DOI: 10.1038/s41467-018-05100-z
  • Peer Reviewed / Open Access
• [Journal Article] Deletion of exons encoding carboxypeptidase domain of Nna1 results in Purkinje cell degeneration (pcd) phenotype (2018)
  • Author(s): Zhou Li、Hossain M. Ibrahim、Yamazaki Maya、Abe Manabu、Natsume Rie、Konno Kohtaro、Kageyama Shun、Komatsu Masaaki、Watanabe Masahiko、Sakimura Kenji、Takebayashi Hirohide
  • Journal Title: Journal of Neurochemistry Volume: 147 Issue: 4 Pages: 557-572
  • DOI: 10.1111/jnc.14591
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distribution of Caskin1 protein and phenotypic characterization of its knockout mice using a comprehensive behavioral test battery (2018)
  • Author(s): Katano Tayo、Takao Keizo、Abe Manabu、Yamazaki Maya、Watanabe Masahiko、Miyakawa Tsuyoshi、Sakimura Kenji、Ito Seiji
  • Journal Title: Molecular Brain Volume: 11 Issue: 1 Pages: 63-63
  • DOI: 10.1186/s13041-018-0407-2
  • Peer Reviewed / Open Access
• [Journal Article] Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis. (2017)
  • Author(s): Yamaguchi J, Suzuki C, Nanao T, Kakuta S, Ozawa K, Tanida I, Saitoh T, Sunabori T, Komatsu M, Tanaka K, Aoki S, Sakimura K, Uchiyama Y.
  • Journal Title: Autophagy Volume: 17 Issue: 5 Pages: 1-14
  • DOI: 10.1080/15548627.2017.1314897
  • NAID: 120006881392
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production (2017)
  • Author(s): Kawai Takafumi、Okochi Yoshifumi、Ozaki Tomohiko、Imura Yoshio、Koizumi Schuichi、Yamazaki Maya、Abe Manabu、Sakimura Kenji、Yamashita Toshihide、Okamura Yasushi
  • Journal Title: Journal of Neurochemistry Volume: 142 Issue: 5 Pages: 686-699
  • DOI: 10.1111/jnc.14106
  • Peer Reviewed / Open Access
• [Journal Article] Retrograde BDNF to TrkB signaling promotes synapse elimination in the developing cerebellum. (2017)
  • Author(s): Choo M, Miyazaki T, Yamazaki M, Kawamura M, Nakazawa T, Zhang J, Tanimura A, Uesaka N, Watanabe M, Sakimura K, Kano M.
  • Journal Title: Nat Commun. Volume: 8(1) Issue: 1 Pages: 195-195
  • DOI: 10.1038/s41467-017-00260-w
  • Peer Reviewed / Open Access
• [Journal Article] Netrin-1 Derived from the Ventricular Zone, but not the Floor Plate, Directs Hindbrain Commissural Axons to the Ventral Midline (2017)
  • Author(s): Yamauchi Kenta、Yamazaki Maya、Abe Manabu、Sakimura Kenji、Lickert Heiko、Kawasaki Takahiko、Murakami Fujio、Hirata Tatsumi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 11992-11992
  • DOI: 10.1038/s41598-017-12269-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Orexin modulates behavioral fear expression through the locus coeruleus (2017)
  • Author(s): Soya Shingo、Takahashi Tohru M.、McHugh Thomas J.、Maejima Takashi、Herlitze Stefan、Abe Manabu、Sakimura Kenji、Sakurai Takeshi
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1606-1606
  • DOI: 10.1038/s41467-017-01782-z
  • NAID: 120007128779
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation. (2017)
  • Author(s): Shimizu T, Osanai Y, Tanaka KF, Abe M, Natsume R, Sakimura K, Ikenaka K.
  • Journal Title: Glia Volume: 65 Issue: 2 Pages: 360-374
  • DOI: 10.1002/glia.23096
  • Peer Reviewed
• [Journal Article] Impaired auditory processing and altered structure of the endbulb of Held synapse in mice lacking the GluA3 subunit of AMPA receptors. (2017)
  • Author(s): Garcia-Hernandez S, Abe M, Sakimura K, Rubio ME.
  • Journal Title: Hear Res. Volume: 344 Pages: 284-294
  • DOI: 10.1016/j.heares.2016.12.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pharmacological properties of SAK3, a novel T-type voltage-gated Ca2+ channel enhancer. (2017)
  • Author(s): Yabuki Y, Matsuo K, Izumi H, Haga H, Yoshida T, Wakamori M, Kakehi A, Sakimura K, Fukuda T, Fukunaga K.
  • Journal Title: Neuropharmacology. Volume: 117 Pages: 1-13
  • DOI: 10.1016/j.neuropharm.2017.01.011
  • Peer Reviewed / Open Access
• [Journal Article] PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts. (2017)
  • Author(s): Danno S, Kubouchi K, Mehruba M, Abe M, Natsume R, Sakimura K, Eguchi S, Oka M, Hirashima M, Yasuda H, Mukai H.
  • Journal Title: Genes Cells. Volume: 22 Issue: 2 Pages: 220-236
  • DOI: 10.1111/gtc.12470
  • Peer Reviewed
• [Journal Article] The balance between Cathepsin C and Cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model. (2017)
  • Author(s): Shimizu T, Wisessmith W, Li J, Abe M, Sakimura K, Chetsawang B, Sahara Y, Tohyama K, Tanaka KF, Ikenaka K
  • Journal Title: Glia Volume: in press Issue: 6 Pages: 917-930
  • DOI: 10.1002/glia.23134
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] TARP γ-2 and γ-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area. (2016)
  • Author(s): Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M
  • Journal Title: J Neurosci Volume: 36 Issue: 15 Pages: 4296-4312
  • DOI: 10.1523/jneurosci.4178-15.2016
  • NAID: 120005849916
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] GluD2 endows parallel fiber-Purkinje cell synapses with a high regenerative capacity. (2016)
  • Author(s): Ichikawa R, Sakimura K, Watanabe M
  • Journal Title: J Neurosci, Volume: 36 Issue: 17 Pages: 4846-4858
  • DOI: 10.1523/jneurosci.0161-16.2016
  • NAID: 120005853072
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Trans-synaptic modulation of kainate receptor functions by C1q-like proteins. (2016)
  • Author(s): Matsuda K, Budisantoso T, Mitakidis N, Sugaya Y, Miura E, Kakegawa W, Yamasaki M, Konno K, Uchigashima M, Abe M, Watanabe I, Kano M, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M.
  • Journal Title: Neuron Volume: in press Issue: 4 Pages: 752-767
  • DOI: 10.1016/j.neuron.2016.04.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice. (2016)
  • Author(s): Konno A, Ikegami K, Konishi Y, Yang HJ, Abe M, Yamazaki M, Sakimura K, Yao I, Shiba K, Inaba K, Setou M.
  • Journal Title: J Cell Sci. Volume: 129 Issue: 14 Pages: 2757-2766
  • DOI: 10.1242/jcs.185983
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells. (2016)
  • Author(s): Yang CC, Suzuki M, Yamakawa S, Uno S, Ishii A, Yamazaki S, Fukatsu R, Fujisawa R, Sakimura K, Tsurimoto T, Masai H.
  • Journal Title: Nat Commun. Volume: 7 Issue: 1 Pages: 12135-12135
  • DOI: 10.1038/ncomms12135
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive. (2016)
  • Author(s): Matsumoto-Makidono, Y., Nakayama, H., Yamasaki, M., Miyazaki, T., Kobayashi, K., Watanabe, M., Kano, M., Sakimura, K., Hashimoto, K.
  • Journal Title: Cell Reports Volume: 16 Issue: 4 Pages: 994-1004
  • DOI: 10.1016/j.celrep.2016.06.053
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crucial roles of the endocannabinoid 2-arachidonoylglycerol in the suppression of epileptic seizures. (2016)
  • Author(s): Sugaya Y, Yamazaki M, Uchigashima M, Kobayashi K, Watanabe M, Sakimura K, Kano M
  • Journal Title: Cell Rep Volume: 16 Issue: 5 Pages: 1405-15
  • DOI: 10.1016/j.celrep.2016.06.083
  • Peer Reviewed / Open Access
• [Journal Article] Microglial phospholipase D4 deficiency influences myelination during brain development (2016)
  • Author(s): Chiba T, Otani Y, Yamaguchi Y, Ishibashi T, Hayashi A, Tanaka KF, Yamazaki M, Sakimura K, Baba H.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 92 Issue: 7 Pages: 237-254
  • DOI: 10.2183/pjab.92.237
  • NAID: 130005253188
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access
• [Journal Article] SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression. (2016)
  • Author(s): Mochida, S.*, Hida, Y., Tanifuji, S., Hagiwara, A., Hamada, S., Abe, M., Huan, M., Yasumura, M., Kitajima, I., Sakimura, K., Ohtsuka, T*.
  • Journal Title: Cell Reports Volume: 16 Issue: 11 Pages: 2901-2913
  • DOI: 10.1016/j.celrep.2016.08.020
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse (2016)
  • Author(s): Kono J,:Konno K, Talukder AH, Fuse T, Abe M, Uchida K, Horio S, Sakimura K, Watanabe M, Itoi K
  • Journal Title: Brain Struc Funct Volume: Epub ahead of print Issue: 1 Pages: 1-28
  • DOI: 10.1007/s00429-016-1303-0
  • Peer Reviewed / Open Access
• [Journal Article] Spatiotemporal dynamics of lesion-induced axonal sprouting and its relation to functional architecture of the cerebellum. (2016)
  • Author(s): Dhar M, Brenner JM, Sakimura K, Kano M, Nishiyama H.
  • Journal Title: Nat Commun. Volume: 7 Issue: 1 Pages: 12938-12938
  • DOI: 10.1038/ncomms12938
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SCOP/PHLPP1β mediates circadian regulation of long-term recongnition memory. (2016)
  • Author(s): Kimiko Shimizu, Yodai Kobayashi, Erika Nakatsuji, Shigeki Shimba, Maya Yamazaki, Kenji Sakimura and Yoshitaka Fukada.
  • Journal Title: Nat. Commun. Volume: 7 Issue: 1 Pages: 12926-12926
  • DOI: 10.1038/ncomms12926
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of novel dystonia musculorum mutant mice: Implications for central nervous system abnormality. (2016)
  • Author(s): Horie M, Mekada K, Sano H, Kikkawa Y, Chiken S, Someya T, Saito K, Hossain MI, Nameta M, Abe K, Sakimura K, Ono K, Nambu A, Yoshiki A, Takebayashi H.
  • Journal Title: Neurobiol Dis. Volume: 96 Pages: 271-283
  • DOI: 10.1016/j.nbd.2016.09.016
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve Injury. (2016)
  • Author(s): Katano T, Fukuda M, Furue H, Yamazaki M, Abe M, Watanabe M, Nishida K, Yao I, Yamada A, Hata Y, Okumura N, Nakazawa T, Yamamoto T, Sakimura K, Takao T, Ito S.
  • Journal Title: eNeuro. Volume: 3 Issue: 5 Pages: 0110-16
  • DOI: 10.1523/eneuro.0110-16.2016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Forward-genetics analysis of sleep in randomly mutagenized mice (2016)
  • Author(s): Funato H and Yanagisawa M et al.
  • Journal Title: Nature Volume: 539(7629) Issue: 46 Pages: 378-383
  • DOI: 10.1038/nature20142
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Roles of Cbln1 in Non-Motor Functions of Mice (2016)
  • Author(s): Otsuka S, Konno K, Abe M, Motohashi J, Kohda K, Sakimura K, Watanabe M, Yuzaki M
  • Journal Title: Journal of Neuroscience Volume: 36 Issue: 46 Pages: 11801-11816
  • DOI: 10.1523/jneurosci.0322-16.2016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Conditional Knockout of Cav2.1 Disrupts the Accuracy of Spatial Recognition of CA1 Place Cells and Spatial/Contextual Recognition Behavior. (2016)
  • Author(s): Jung D, Hwang YJ, Ryu H, Kano M, Sakimura K, Cho J.
  • Journal Title: Front Behav Neurosci. Volume: 10 Pages: 214-214
  • DOI: 10.3389/fnbeh.2016.00214
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] C57BL/6系統マウスにおける卵管エレクトロポレーション法を用いた遺伝子編集効率の検討 (2019)
  • Author(s): 夏目里恵、中務胞、崎村建司、阿部学
  • Organizer: 第66回日本実験動物学会総会
• [Presentation] 自然発症ミュータントラットからのナイーブES細胞の樹立 (2019)
  • Author(s): 中務胞、夏目里恵、阿部学、崎村建司
  • Organizer: 第66回日本実験動物学会総会
• [Presentation] The development of gene-manipulated animal models: Aiming to generate better suited mouse, rat and marmoset for the analysis of brain function (2018)
  • Author(s): Sakimura K
  • Organizer: 8th BRI International Symposium
  • Int'l Joint Research
• [Presentation] 卵管膨大部エレクトロポレーション法を用いた遺伝子組換えマウス作製の迅速化 (2018)
  • Author(s): 夏目里恵、阿部学、中務胞、崎村建司
  • Organizer: 第65回日本実験動物学会総会
• [Presentation] Extracerebellar expression of glutamate receptor GluD2 in adult mice and marmosets (2018)
  • Author(s): Watanabe M, Nakamoto C, Sakimura K, Kano M, Konno K
  • Organizer: 11th FENS Forum of Neuroscience
  • Int'l Joint Research
• [Presentation] 遺伝子改変ホモマウスへの過剰排卵処置方法の検討 (2018)
  • Author(s): 中務胞、夏目里恵、阿部学、崎村建司
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] Roles of RNA editing in neuropathic pain after peripheral nerve injury 神経障害性疼痛におけるＲＮＡ編集異常とその役割 (2017)
  • Author(s): 内田仁司、崎村建司、伊藤誠二
  • Organizer: 第90会日本薬理学会
  • Place of Presentation: 長崎ブリックホール（長崎県長崎市）
  • Year and Date: 2017-03-16
  • Int'l Joint Research
• [Presentation] CARD HyperOvaを用いたBNラットの過剰排卵誘起処置の有用性 (2017)
  • Author(s): 中務胞、夏目里恵、崎村建司
  • Organizer: 第64回日本実験動物学会総会
• [Presentation] GluD1欠損マウスにみられるうつ様行動は抗うつ薬により回復する (2017)
  • Author(s): 中本千尋、夏目里恵、阿部学、川村名子、内田仁司、渡辺雅彦、崎村建司
  • Organizer: 第40回日本神経科学大会
• [Presentation] RNA編集酵素ADAR2は神経障害性疼痛に関与する (2017)
  • Author(s): 内田仁司、崎村建司、伊藤誠二
  • Organizer: 第40回日本神経科学大会
• [Presentation] GluD1 deficient mouse as a model animal of depression-like behavior (2017)
  • Author(s): Nakamoto C, Natsume R, Abe M, Kawamura M, Uchida H, Watanabe M, Sakimura K
  • Organizer: 26th ISN-ESN Biennial Meeting
  • Int'l Joint Research
• [Presentation] Regulatory mechanism of hypothalamic corticotropin-releasing factor neurons by the ascending monoaminergic systems (2017)
  • Author(s): Itoi K, Sakimura K
  • Organizer: 第60回日本神経化学会大会
• [Presentation] Aif1-iCre knock-in mouse line: A tool for conditional gene manipulation in microglia (2017)
  • Author(s): Abe M, Peng F, Sakimura K
  • Organizer: SfN's 47th Annual Meeting Neuroscience 2017
  • Int'l Joint Research
• [Presentation] ラット卵巣卵胞内卵子の成熟培養なしでの体外受精 (2017)
  • Author(s): 中務胞、夏目里恵、崎村建司
  • Organizer: 第40回日本分子生物学会年会 (ConBio2017)
• [Presentation] 発生および慢性疼痛下の一次知覚神経におけるRNA編集の変動 (2017)
  • Author(s): 内田仁司、松村伸治、崎村建司、伊藤誠二
  • Organizer: 第40回日本分子生物学会年会 (ConBio2017)
• [Presentation] ラットES細胞を用いた遺伝子改変動物作製の効率化：妊娠維持障害の改善 (2016)
  • Author(s): 中務胞、夏目里恵、崎村建司
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-12-01
  • Int'l Joint Research
• [Presentation] Low-affinity kainate receptor subunit, gluk1, gluK2 and gluK3, are responsible for mouse anxiety-like behavior. (2016)
  • Author(s): Izumi Watanabe-Iida, K.Konno, M.Abe, R.Natsume, M.Watanabe, K.Sakimura
  • Organizer: Neuroscience2016
  • Place of Presentation: SanDiego (USA)
  • Year and Date: 2016-11-13
  • Int'l Joint Research
• [Presentation] Distribution of cortictropin-releasing factor neurons in the mouse brain: a study using cortisotropin-releasing factor modified yellow fluorescent protein knock-in mouse (2016)
  • Author(s): K.Itoi, J.Kono, K. Konno, A.H.Talukder, T.Fuse, K.Uchida, S.Yamagata, S.Horio,M.Abe, M.Watanabe, K.Sakimura
  • Organizer: Neuroscience2016
  • Place of Presentation: SanDiego (USA)
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] デルタ型グルタミン酸受容体サブユニット機能とその量的関連 (2016)
  • Author(s): 中本千尋、川村名子、夏目里恵、阿部学、渡辺雅彦、崎村建司
  • Organizer: 第59回日本神経化学会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-08
  • Int'l Joint Research
• [Presentation] マウス受精卵電気穿孔法を用いたCRISPR/Cas9 システムによる内在性タンパク質へのタグ付加の試み / CRISPR/Cas9-mediated endogenenous protein tagging in mice by zygote electroporation (2016)
  • Author(s): 阿部学、夏目里恵、中務胞、崎村建司
  • Organizer: 第1回 ゲノム編集学会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] GLuD1 and Cbln1 interation plays an important role in synapse formation between particular neurochemical nucleus and dorsolateral geniculate nucleus (2016)
  • Author(s): Kohtaro Konno, Koji Nishikawa, Shinji Yamada, Michisuke Yuzaki, Kenji Sakimura, Masahiko Watanabe
  • Organizer: 第39回日本神経科学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-22
  • Int'l Joint Research
• [Presentation] Mapping of coticortropin-releasing factoe neurons in mouse brain: a study using a modified yellow flourescent protein knock-in mouse (2016)
  • Author(s): Keiichi Itoi, Junko Kono, Kotaro Konno, Talukder H. Ashraf, Katsuya Uchida, Toshimitsu Fuse, Manabu Abe, Rie Natsume, Shuhei Horio, Kenji Sakimura,Masahiko Watanabe
  • Organizer: 第39回日本神経科学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
  • Int'l Joint Research
• [Presentation] GluN3A colocalizes with KV4.3 at non-synaptic climbing fiber-interneuron junctions in the cerebellum (2016)
  • Author(s): Xiaohong Song, Masahiro Fukaya, Miwako Yamasaki, Motokazu Uchigashima, Kohtaro Konno, Taisuke Miyazaki, Keiko Matsuda, Chihiro Nakamoto, Isabel Perez-Otano, Michisuke Yuzaki, Kenji Sakimura, Masahiko Watanabe
  • Organizer: 第39回日本神経科学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
  • Int'l Joint Research
• [Presentation] ラットES細胞を用いた遺伝子改変動物作製の効率化：受容胚系統の比較 (2016)
  • Author(s): 中務胞、夏目里恵、崎村建司
  • Organizer: 第63回日本実験動物学会総会
  • Place of Presentation: ミューザ川崎（神奈川県川崎市）
  • Year and Date: 2016-05-20
  • Int'l Joint Research
• [Remarks] 新潟大学脳研究所Webページ
  • URL: http://www.bri.niigata-u.ac.jp
• [Remarks] メカニカルストレスが脳細胞へ与える影響の一端を解明
  • URL: http://www.bri.niigata-u.ac.jp/result/cellular/000675.html