Heterochromatin dynamics revealed by single nucleosome imaging
| Project/Area Number |
16H04746
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
Maeshima Kazuhiro 国立遺伝学研究所, 遺伝メカニズム研究系, 教授 (00392118)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | クロマチン動態 / 染色体 / ヌクレオソーム / 1分子イメージング |
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| Outline of Final Research Achievements |
How is two meters of genome DNA three-dimensionally organized in the cell as chromatin? For this ten years, we have revealed that chromatin has a dynamic and highly variable configuration like a fluid, rather than a static regular structure like a crystal. If chromatin is fluid-like, what is the organization of heterochromatin, which is assumed to be more condensed, silenced and inactive? We hypothesized that local chromatin dynamics are suppressed in heterochromatin regions, causing a limited access to the regions. We investigated the nucleosome motion in heterochromatin by single nucleosome imaging and fluorescent nucleotide labeling, and proved our hypothesis. We suggested that heterochromatin, whose nucleosomes are crosslinked by some specific chromatin proteins, has lower local mobility and causes lower accessibility of the genome regions, contributing to their silencing.
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| Academic Significance and Societal Importance of the Research Achievements |
個々のヌクレオソームの動きは、クロマチンへのアクセシビリティの制御とも直結し、遺伝子発現調節、DNA 複製、染色体構築のような関連領域に大きなインパクトを与える。その学術的意義は大きい。また、ヒトゲノム情報は私たちの健康や生命に深く関わっている。本研究課題の成果は、「私たちの生命に関わる細胞内のゲノム情報がどのように整理され、アクセスされ、読み出されるのか？」という疑問の解決に直結するものであり、その社会的意義は大きい。
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] The 10-nm Chromatin Fiber and its Relationship to Interphase Chromosome Organization.2018
Author(s)
Hansen, J.C., Connolly, M., McDonald, C.J., Pan, A., Pryamkova, A., Ray, K., Seidel, E., Tamura, S., Rogge, R., Maeshima, K.
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Journal Title
Biochemical Society Transactions
Volume: 46
Issue: 1
Pages: 67-76
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Density imaging of heterochromatin in live cells using orientation-independent-DIC microscopy.2017
Author(s)
Imai, R., Nozaki, T., Tani, T., Kaizu, K., Hibino, K., Ide, S., Tamura, S., Takahashi, K., Shribak, M., and Maeshima, K.
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Journal Title
Molecular Biology of the Cell.
Volume: 28
Issue: 23
Pages: 3349-3359
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Dynamic organization of chromatin domains revealed by super-resolution live-cell imaging.2017
Author(s)
Nozaki, T., Imai, R., Tanbo, M., Nagashima, R., Tamura, S., Tani, T., Joti, Y., Tomita, M., Hibino, K., Wendt, K.S., Okada, Y., Nagai, T., Maeshima, K.
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Journal Title
Molecular Cell.
Volume: 67
Issue: 2
Pages: 282-293
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole-Imidazole Polyamide Probes.2016
Author(s)
Kawamoto, Y.,Sasaki, A., Chandrana, A., Hashiya, K., Ide, S., Bando, T., Maeshima, K., Sugiyama, H.
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Journal Title
Journal of the American Chemical Society
Volume: 138
Issue: 42
Pages: 14100-14107
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Budding yeast chromatin is dispersed in a crowded nucleoplasm.2016
Author(s)
Chen, C., Hwa Lim, H., Shi, J., Tamura, S., Maeshima, K., Surana, U., and Gan, L.
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Journal Title
Molecular Biology of the Cell.
Volume: 27
Issue: 21
Pages: 3357-3368
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Telomere Visualization in Tissue Sections Using Pyrrole-Imidazole Polyamide Probes.2016
Author(s)
Sasaki, A., Ide, S., Kawamoto, Y., Bando, T., Murata, Y., Shimura, M., Yamada, K., Hirata, A., Nokihara, K., Hirata, T., Sugiyama, H., Maeshima, K.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 29261-29261
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nucleosomal arrays self-assemble into supramolecular globular structures lacking 30-nm fibers.2016
Author(s)
Kazuhiro Maeshima, Ryan Rogge, Sachiko Tamura, Yasumasa Joti, Takaaki Hikima, Heather Szerlong, Christine Krause, Jake Herman, Jennifer DeLuca, Tetsuya Ishikawa, Jeffrey C. Hansen
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Journal Title
EMBO J.
Volume: in press
Issue: 10
Pages: 1115-1132
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Chromatin folding and DNA replication inhibition mediated by a highly antitumor-active tetrazolato-bridged dinuclear platinum(II) complex.2016
Author(s)
Imai R, Komeda S, Shimura M, Tamura S, Matsuyama S, Nishimura K, Rogge R, Matsunaga A, Hiratani I, Takata H, Uemura M, Iida Y, Yoshikawa Y, Hansen JC, Yamauchi K, Kanemaki MT, Maeshima K.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 24712-24725
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Book] 少数性生物学2017
Author(s)
前島一博
Total Pages
8
Publisher
日本評論社
ISBN
9784535788169
Related Report
