Structural biology study on the interactions between lectins and branched glycans

• Project/Area Number: 16H04758
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Tohoku Medical and Pharmaceutical University (2018-2019); Institute of Physical and Chemical Research (2016-2017)
• Principal Investigator: Yamaguchi Yoshiki 東北医科薬科大学, 薬学部, 教授 (90323451)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 糖鎖 / レクチン / X線結晶構造解析 / NMR / 分子動力学 / ダイナミクス / βグルカン / レクチン受容体 / SEC-MALS / オリゴマー / イオンモビリティ / 衝突断面積 / 分子動力学計算

Outline of Final Research Achievements

Branched glycans attached to proteins play many diverse roles in protein quality control and cell-cell communication etc. Many of the glycan functions are attained through interactions with lectin receptors. However, we still do not know much about the 3D structure and interaction in detail. In this study, we aimed to understand the role of each glycan branch in interacting with lectin receptors. To attain this, we used “branched” glycan unit, not “linear” oligosaccharide as lectin ligands. Here we revealed that lectin receptors are categorized into two types, one binds to both branches irrespective of the branch position, and the other selectively binds to one of the glycan branches.

Academic Significance and Societal Importance of the Research Achievements

糖鎖の分岐構造の生物学的意義については、その多価性によるレクチン受容体とのみかけの親和性の増大が従来報告されてきた。しかしながら、分岐構造の性質や生物学的役割の違についての理解はあまり進んでいなかった。本研究の遂行により、レクチン受容体との相互作用における分岐糖鎖の各分枝の役割の一旦を構造生物学的に解明することに成功した。得られた知見は、生理活性を示す糖鎖誘導体の開発（ワクチン、アジュバント）やレクチン受容体をターゲットとした創薬研究への貢献につながる。

Research Products

• [Journal Article] Lectin recognition and signaling mechanism in relation to glycan diversity (2018)
  • Author(s): 長江 雅倫、山口 芳樹
  • Journal Title: 生化学 Volume: 90 Issue: 5 Pages: 651-663
  • DOI: 10.14952/SEIKAGAKU.2018.900651
  • NAID: 40021722701
  • ISSN: 0037-1017
  • Year and Date: 2018-10-25
  • Peer Reviewed
• [Journal Article] Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs (2018)
  • Author(s): Nakajima Kazuki、Kizuka Yasuhiko、Yamaguchi Yoshiki、Hirabayashi Yoshio、Takahashi Kazuo、Yuzawa Yukio、Taniguchi Naoyuki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 1 Pages: 401-407
  • DOI: 10.1016/j.bbrc.2017.10.173
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Bisecting GlcNAc restricts conformations of branches in model N -glycans with GlcNAc termini (2018)
  • Author(s): Hanashima Shinya、Suga Akitsugu、Yamaguchi Yoshiki
  • Journal Title: Carbohydrate Research Volume: 456 Pages: 53-60
  • DOI: 10.1016/j.carres.2017.12.002
  • Peer Reviewed
• [Journal Article] Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase (2018)
  • Author(s): Re Suyong、Watabe Shigehisa、Nishima Wataru、Muneyuki Eiro、Yamaguchi Yoshiki、MacKerell Alexander D.、Sugita Yuji
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1644-1644
  • DOI: 10.1038/s41598-018-20012-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of a Golgi GPI-N-acetylgalactosamine transferase with tandem transmembrane regions in the catalytic domain (2018)
  • Author(s): Hirata Tetsuya、Mishra Sushil K.、Nakamura Shota、Saito Kazunobu、Motooka Daisuke、Takada Yoko、Kanzawa Noriyuki、Murakami Yoshiko、Maeda Yusuke、Fujita Morihisa、Yamaguchi Yoshiki、Kinoshita Taroh
  • Journal Title: Nature Coomunications Volume: 9 Issue: 1 Pages: 405-405
  • DOI: 10.1038/s41467-017-02799-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis of protein arginine rhamnosylation by glycosyltransferase EarP (2018)
  • Author(s): Sengoku Toru、Suzuki Takehiro、Dohmae Naoshi、Watanabe Chiduru、Honma Teruki、Hikida Yasushi、Yamaguchi Yoshiki、Takahashi Hideyuki、Yokoyama Shigeyuki、Yanagisawa Tatsuo
  • Journal Title: Nature Chemical Biology Volume: 14 Issue: 4 Pages: 368-374
  • DOI: 10.1038/s41589-018-0002-y
  • Peer Reviewed
• [Journal Article] Molecular basis for diversification of yeast prion strain conformation (2018)
  • Author(s): Ohhashi Yumiko、Yamaguchi Yoshiki、Kurahashi Hiroshi、Kamatari Yuji O.、Sugiyama Shinju、Uluca Boran、Piechatzek Timo、Komi Yusuke、Shida Toshinobu、M?ller Henrik、Hanashima Shinya、Heise Henrike、Kuwata Kazuo、Tanaka Motomasa
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 10 Pages: 2389-2394
  • DOI: 10.1073/pnas.1715483115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spontaneous intracranial hypotension is diagnosed by a combination of lipocalin-type prostaglandin D synthase and brain-type transferrin in cerebrospinal fluid (2018)
  • Author(s): Yuta Murakami, Koichi Takahashi, Kyoka Hoshi, Hiromi Ito, Mayumi Kanno, Kiyoshi Saito, Kenneth Nollet, Yoshiki Yamaguchi, Masakazu Miyajima, Hajime Arai, Yasuhiro Hashimoto, Tatsuo Mima
  • Journal Title: BBA General Subjects Volume: 1862 Issue: 8 Pages: 1835-1842
  • DOI: 10.1016/j.bbagen.2018.03.027
  • Peer Reviewed
• [Journal Article] Biological role of site-specific O-glycosylation in cell adhesion activity and phosphorylation of osteopontin (2018)
  • Author(s): Oyama Midori、Kariya Yoshinobu、Kariya Yukiko、Matsumoto Kana、Kanno Mayumi、Yamaguchi Yoshiki、Hashimoto Yasuhiro
  • Journal Title: Biochemical Journal Volume: 475 Issue: 9 Pages: 1583-1595
  • DOI: 10.1042/bcj20170205
  • Peer Reviewed
• [Journal Article] High affinity sugar ligands of C-type lectin receptor langerin. (2018)
  • Author(s): Ota F, Hirayama T, Kizuka Y, Yamaguchi Y, Fujinawa R, Nagata M; Ismanto HS, Lepenies, Aretz J, Rademacher C, Seeberger PH; Angata T, Kitazume S, Yoshida K, Betsuyaku T, Kida K Yamasaki S, and Taniguchi N
  • Journal Title: BBA - General Subjects Volume: - Issue: 7 Pages: 1592-1601
  • DOI: 10.1016/j.bbagen.2018.04.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] J(77Se,1H) and J(77Se,13C) couplings of seleno-carbohydrates obtained by 77Se satellite 1D 13C spectroscopy and 77Se selective HR-HMBC spectroscopy (2018)
  • Author(s): Uzawa Jun、Shimabukuro Junpei、Suzuki Tatsuya、Imamura Akihiro、Ishida Hideharu、Ando Hiromune、Yamaguchi Yoshiki
  • Journal Title: Magnetic Resonance in Chemistry Volume: 56 Issue: 9 Pages: 836-846
  • DOI: 10.1002/mrc.4746
  • Peer Reviewed / Open Access
• [Journal Article] Rapid increase of 'brain-type' transferrin in cerebrospinal fluid after shunt surgery for idiopathic normal pressure hydrocephalus: a prognosis marker for cognitive recovery. (2018)
  • Author(s): Murakami Y, Matsumoto Y, Hoshi K, Ito H, Fuwa TJ, Yamaguchi Y, Nakajima M, Miyajima M, Arai H, Nollet K, Kato N, Nishikata R, Kuroda N, Honda T, Sakuma J, Saito K, Hashimoto Y.
  • Journal Title: J Biochem. Volume: 164 Issue: 3 Pages: 205-213
  • DOI: 10.1093/jb/mvy043
  • NAID: 40021678439
  • Peer Reviewed / Open Access
• [Journal Article] Acceptor range of endo-β-N-acetylglucosaminidase mutant endo-CC N180H: from monosaccharide to antibody (2018)
  • Author(s): Manabe Shino、Yamaguchi Yoshiki、Abe Junpei、Matsumoto Kana、Ito Yukishige
  • Journal Title: Royal Society Open Science Volume: 5 Issue: 5 Pages: 171521-171521
  • DOI: 10.1098/rsos.171521
  • Peer Reviewed / Open Access
• [Journal Article] β-Glucan-induced cooperative oligomerization of Dectin-1 C-type lectin-like domain (2018)
  • Author(s): Dulal Hari P、Adachi Yoshiyuki、Ohno Naohito、Yamaguchi Yoshiki
  • Journal Title: Glycobiology Volume: 28 Issue: 8 Pages: 612-623
  • DOI: 10.1093/glycob/cwy039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Analysis of protein landscapes around N-glycosylation sites from the PDB repository for understanding the structural basis of N-glycoprotein processing and maturation (2018)
  • Author(s): Suga Akitsugu、Nagae Masamichi、Yamaguchi Yoshiki
  • Journal Title: Glycobiology Volume: 28 Issue: 10 Pages: 774-785
  • DOI: 10.1093/glycob/cwy059
  • Peer Reviewed
• [Journal Article] Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V (2018)
  • Author(s): Nagae Masamichi、Kizuka Yasuhiko、Mihara Emiko、Kitago Yu、Hanashima Shinya、Ito Yukishige、Takagi Junichi、Taniguchi Naoyuki、Yamaguchi Yoshiki
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 3380-3380
  • DOI: 10.1038/s41467-018-05931-w
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis of the Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni : A Putative Cause of Guillain?Barr? Syndrome (2018)
  • Author(s): Yoshida Fumi、Yoshinaka Hiroki、Tanaka Hidenori、Hanashima Shinya、Yamaguchi Yoshiki、Ishihara Mikio、Saburomaru Miyuki、Kato Yuki、Saito Risa、Ando Hiromune、Kiso Makoto、Imamura Akihiro、Ishida Hideharu
  • Journal Title: Chemistry A European Journal Volume: 25 Issue: 3 Pages: 796-805
  • DOI: 10.1002/chem.201804862
  • Peer Reviewed / Open Access
• [Journal Article] Tau Filaments and the Development of Positron Emission Tomography Tracers (2018)
  • Author(s): Goedert Michel、Yamaguchi Yoshiki、Mishra Sushil K.、Higuchi Makoto、Sahara Naruhiko
  • Journal Title: Frontiers in Neurology Volume: 9 Pages: 70-70
  • DOI: 10.3389/fneur.2018.00070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Implication of C-type lectin receptor langerin and keratan sulfate disaccharide in emphysema (2018)
  • Author(s): Kizuka Yasuhiko、Mishra Sushil、Yamaguchi Yoshiki、Taniguchi Naoyuki
  • Journal Title: Cellular Immunology Volume: 333 Pages: 80-84
  • DOI: 10.1016/j.cellimm.2018.07.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biophysical Analyses for Probing Glycan-Protein Interactions (2018)
  • Author(s): Nagae Masamichi、Yamaguchi Yoshiki
  • Journal Title: Adv. Exp. Med. Biol. Volume: 1104 Pages: 119-147
  • DOI: 10.1007/978-981-13-2158-0_7
  • ISBN: 9789811321573, 9789811321580
  • Peer Reviewed / Open Access
• [Journal Article] Distinct roles for each N-glycan branch interacting with mannose-binding type Jacalin-related lectins Orysata and Calsepa. (2017)
  • Author(s): Nagae M, Mishra SK, Hanashima S, Tateno H, Yamaguchi Y
  • Journal Title: Glycobiology Volume: 27(12) Issue: 12 Pages: 1120-1133
  • DOI: 10.1093/glycob/cwx081
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A keratan sulfate disaccharide prevents inflammation and the progression of emphysema in murine models. (2017)
  • Author(s): Gao C, Fujinawa R, Yoshida T, Ueno M, Ota F, Kizuka Y, Hirayama T, Korekane H, Kitazume S, Maeno T, Ohtsubo K, Yoshida K, Yamaguchi Y, Lepenies B, Aretz J, Rademacher C, Kabata H, Hegab AE, Seeberger PH, Betsuyaku T, Kida K, Taniguchi N.
  • Journal Title: American Journal of Physiology Lung Cellular and Molecular Physiology Volume: 312 Issue: 2 Pages: L268-L276
  • DOI: 10.1152/ajplung.00151.2016
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sialylation of extracellular superoxide dismutase (EC-SOD) enhances furin-mediated cleavage and secretion (2017)
  • Author(s): Ota Fumi、Kizuka Yasuhiko、Nakano Miyako、Yamaguchi Yoshiki、Kitazume Shinobu、Ookawara Tomomi、Taniguchi Naoyuki
  • Journal Title: Glycobiology Volume: 27 Issue: 12 Pages: 1081-1088
  • DOI: 10.1093/glycob/cwx087
  • Peer Reviewed / Open Access
• [Journal Article] An Alkynyl-Fucose Halts Hepatoma Cell Migration and Invasion by Inhibiting GDP-Fucose-Synthesizing Enzyme FX, TSTA3 (2017)
  • Author(s): Kizuka Yasuhiko、Nakano Miyako、Yamaguchi Yoshiki、Nakajima Kazuki、Oka Ritsuko、Sato Keiko、Ren Chien-Tai、Hsu Tsui-Ling、Wong Chi-Huey、Taniguchi Naoyuki
  • Journal Title: Cell Chemical Biology Volume: 24 Issue: 12 Pages: 1467-1478
  • DOI: 10.1016/j.chembiol.2017.08.023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Synthesis of a biotinylated keratan sulfate tetrasaccharide composed of dimeric Galβ1-4GlcNAc6Sβ (2017)
  • Author(s): Takeda-Okuda Naoko、Yamaguchi Yoshiki、Uzawa Jun、Tamura Jun-ichi
  • Journal Title: Carbohydrate Research Volume: 452 Pages: 97-107
  • DOI: 10.1016/j.carres.2017.09.005
  • Peer Reviewed / Open Access
• [Journal Article] A unique glycan-isoform of transferrin in cerebrospinal fluid: A potential diagnostic marker for neurological diseases (2017)
  • Author(s): Hoshi Kyoka、Matsumoto Yuka、Ito Hiromi、Saito Kiyoshi、Honda Takashi、Yamaguchi Yoshiki、Hashimoto Yasuhiro
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1861 Issue: 10 Pages: 2473-2478
  • DOI: 10.1016/j.bbagen.2017.07.005
  • Peer Reviewed
• [Journal Article] Novel missense mutation in DLL4 in a Japanese sporadic case of Adams-Oliver syndrome. (2017)
  • Author(s): Taniguchi-Ikeda M, Nagasaka M, Inagaki H, Ouchi Y, Kurokawa D, Yamana K, Harada R, Nozu K, Sakai Y, Mishra SK, Yamaguchi Y, Morikoka I, Toda T, Kurahashi H, Iijima K.
  • Journal Title: Journal of Human Genetics Volume: 未定 Issue: 9 Pages: 851-855
  • DOI: 10.1038/jhg.2017.48
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches (2017)
  • Author(s): K. Usachev, Y. Yamaguchi, M. Takamatsu, N. Pavlova, V. Klochkov, A. Kurbangalieva, T. Murase, T. Shimoda, K. Tanaka
  • Journal Title: Analyst Volume: 142 Issue: 16 Pages: 2897-2900
  • DOI: 10.1039/c7an00817a
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disruption of the structural and functional features of surfactant protein A by acrolein in cigarette smoke (2017)
  • Author(s): Takamiya Rina、Uchida Koji、Shibata Takahiro、Maeno Toshitaka、Kato Masaki、Yamaguchi Yoshiki、Ariki Shigeru、Hasegawa Yoshihiro、Saito Atsushi、Miwa Soichi、Takahashi Hiroki、Akaike Takaaki、Kuroki Yoshio、Takahashi Motoko
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 8304-8304
  • DOI: 10.1038/s41598-017-08588-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reactivity of anti-HNK-1 antibodies to branched O-mannose glycans associated with demyelination (2017)
  • Author(s): K. Sakuda, Y. Kizuka, Y. Yamaguchi, K. Tanaka, K. Ogiwara, T. Segawa, Y. Hagiwara, I. Matsuo, H. Ogawa, N. Taniguchi, S. Kitazume
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 487 Issue: 2 Pages: 450-456
  • DOI: 10.1016/j.bbrc.2017.04.085
  • Peer Reviewed / Open Access
• [Journal Article] Crystallographic analysis of murine p24γ2 Golgi dynamics domain (2017)
  • Author(s): Nagae M, Liebschner D, Yamada Y, Morita-Matsumoto K, Matsugaki N, Senda T, Fujita M, Kinoshita T, Yamaguchi Y
  • Journal Title: Proteins Volume: 85 Issue: 4 Pages: 764-70
  • DOI: 10.1002/prot.25242
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 3D structural analysis of protein O-mannosyl kinase, POMK, a causative gene product of dystroglycanopathy (2017)
  • Author(s): M.Nagae, S.K.Mishra, M.Neyazaki, R.Oi, A.Ikeda, N.Matsugaki, S.Akashi, H.Manya, M.Mizuno, H.Yagi, K.Kato, T.Senda, T.Endo, T.Nogi, and Y.Yamaguchi
  • Journal Title: Genes Cells Volume: - Issue: 4 Pages: 348-359
  • DOI: 10.1111/gtc.12480
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of blood-brain barrier function by quotient alpha2 macroglobulin and its relationship with interleukin-6 and complement component 3 levels in neuropsychiatric systemic lupus erythematosus (2017)
  • Author(s): Asano Tomoyuki、Ito Hiromi、Kariya Yoshinobu、Hoshi Kyoka、Yoshihara Akioh、Ugawa Yoshikazu、Sekine Hideharu、Hirohata Shunsei、Yamaguchi Yoshiki、Sato Shuzo、Kobayashi Hiroko、Migita Kiyoshi、Ohira Hiromasa、Hashimoto Yasuhiro、Watanabe Hiroshi
  • Journal Title: PLOS ONE Volume: 12 Issue: 10 Pages: e0186414-e0186414
  • DOI: 10.1371/journal.pone.0186414
  • Peer Reviewed / Open Access
• [Journal Article] 1,5-Diazacyclooctanes, as Exclusive Oxidative Polyamine Metabolites, Inhibit Amyloid-β(1-40) 10. 1,5-Diazacyclooctanes, as Exclusive Oxidative Polyamine Metabolites, Inhibit Amyloid-β(1-40) Fibrillization (2016)
  • Author(s): A. Tsutsui, T. Zako, T. Bu, Y. Yamaguchi, M. Maeda, K. Tanaka
  • Journal Title: Adv. Sci. Volume: 3 Issue: 10 Pages: 1600082-1600082
  • DOI: 10.1002/advs.201600082
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Aglycon diversity of brain sterylglucosides: Structure determination of cholesteryl- and sitosterylglucoside. (2016)
  • Author(s): Akiyama H, Nakajima K, Itoh Y, Sayano T, Ohashi Y, Yamaguchi Y, Greimel, P, Hirabayashi Y.
  • Journal Title: J Lipid Res. Volume: 57 Issue: 11 Pages: 2061-72
  • DOI: 10.1194/jlr.m071480
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The muscular dystrophy gene TMEM5encodes a ribitol β1-4 Xylosyltransferase required for the functional glycosylation of dystroglycan. (2016)
  • Author(s): Manya, H., Yamaguchi, Y., Kanagawa, M., Kobayashi, K., Tajiri, M., Akasaka-Manya, K., Kawakami, H., Mizuno, M., Wada, Y., Toda, T., and Endo, T.
  • Journal Title: J. Biol. Chem Volume: 291 Issue: 47 Pages: 24618-24627
  • DOI: 10.1074/jbc.m116.751917
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 3D Structure and Interaction of p24β and p24δ Golgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport. (2016)
  • Author(s): Nagae M, Hirata T, Morita-Matsumoto K, Theiler R, Fujita M, Kinoshita T, Yamaguchi Y.
  • Journal Title: J Mol Biol. Volume: 428 Issue: 20 Pages: 4087-99
  • DOI: 10.1016/j.jmb.2016.08.023
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Polyclonal IgM and IgA block in vitro complement deposition mediated by anti-ganglioside antibodies in autoimmune neuropathies (2016)
  • Author(s): Sudo Makoto、Miyaji Kazuki、Späth Peter J.、Morita-Matsumoto Kana、Yamaguchi Yoshiki、Yuki Nobuhiro
  • Journal Title: International Immunopharmacology Volume: 40 Pages: 11-15
  • DOI: 10.1016/j.intimp.2016.08.019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 6-Azido-6-deoxy-L-idose as a hetero-bifunctional spacer for the synthesis of zido-containing chemical probes (2016)
  • Author(s): Hamagami, H., Kumazoe, M., Yamaguchi,Y., Fuse,S., Tachibana, H., and Tanaka, H.
  • Journal Title: Chemistry - Eur. J. Volume: 22 Issue: 36 Pages: 12884-12890
  • DOI: 10.1002/chem.201602044
  • Peer Reviewed
• [Journal Article] High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery. (2016)
  • Author(s): Kizuka Y, Funayama S, Shogomori H, Nakano M, Nakajima K, Oka R, Kitazume S, Yamaguchi Y, Sano M, Korekane H, Hsu TL, Lee HY, Wong CH, Taniguchi N.
  • Journal Title: Cell Chemical Biology Volume: 23 Issue: 7 Pages: 782-792
  • DOI: 10.1016/j.chembiol.2016.06.010
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Enhancement of solubility and yield of a β-glucan receptor Dectin-1 C-type lectin-like domain in Escherichia coli with a solubility-enhancement tag. Protein (2016)
  • Author(s): Dulal HP, Nagae M, Ikeda A, Morita-Matsumoto K, Adachi Y, Ohno N, Yamaguchi Y.
  • Journal Title: Expr Purif. Volume: 123 Pages: 97-104
  • DOI: 10.1016/j.pep.2016.04.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The α-Glycosidation of Partially Unprotected N-Acetyl and N-Glycolyl Sialyl Donors in the Absence of a Nitrile Solvent Effect (2016)
  • Author(s): Taku Aoyagi, Shuichi Ohira, Shinichiro Fuse, Jun Uzawa, Yoshiki Yamaguchi, Hiroshi Tanaka
  • Journal Title: Chemistry A Europian Jounal Volume: 22 Issue: 20 Pages: 6968-6973
  • DOI: 10.1002/chem.201601031
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Crystal structure of human dendritic cell inhibitory receptor (DCIR) C-type lectin domain reveals the binding mode with N-glycan (2016)
  • Author(s): Nagae M, Ikeda A, Hanashima S, Kojima T, Matsumoto N, Yamamoto K, Yamaguchi Y.
  • Journal Title: FEBS Lett. Volume: Mar 26 Issue: 8 Pages: 1-9
  • DOI: 10.1002/1873-3468.12162
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Subgroup differences in “brain-type” transferrin and alpha-synuclein in Parkinson's disease and multiple system atrophy (2016)
  • Author(s): A Yoshihara, M Fukatsu, K Hoshi, Y Hashimoto, et. al
  • Journal Title: The Journal of Biochemistry Volume: 159 Issue: 2 Pages: 87-91
  • DOI: 10.1093/jb/mvw015
  • Description: 未定
  • Peer Reviewed
• [Presentation] タンパク質凝集体とリガンドのNMR相互作用解析と計算化学の役割 (2018)
  • Author(s): 山口芳樹
  • Organizer: よこはまNMR研究会 第５９回ワークショップ
  • Invited
• [Presentation] 均一な抗体－薬物複合体（ADC）の開発に向けて－糖鎖改変技術の利用－ (2018)
  • Author(s): 山口芳樹
  • Organizer: 第４３回理研イブニングセミナー
• [Presentation] 糖鎖がもたらす生物機能の探求 (2018)
  • Author(s): 山口芳樹
  • Organizer: 徳島大学セミナー
• [Presentation] 糖鎖リガンドの動的構造とレクチン受容体との相互作用 (2018)
  • Author(s): 山口芳樹
  • Organizer: 若手NMR研究会
• [Presentation] βグルカン受容体のリガンド結合メカニズム (2018)
  • Author(s): 山口芳樹
  • Organizer: 日本医真菌学会シンポジウム
  • Invited
• [Presentation] レクチン受容体による糖鎖認識とシグナリングの構造生物学 (2018)
  • Author(s): 山口芳樹
  • Organizer: 日本生化学会大会シンポジウム
  • Invited
• [Presentation] Regulation of glycan structure and its recognition by lectin receptors (2018)
  • Author(s): Yoshiki Yamaguch
  • Organizer: 10th ACGG, Tainan
  • Int'l Joint Research / Invited
• [Presentation] ありふれた糖鎖がシグナルになる秘密：CLEC-2の立体構造から学ぶ (2017)
  • Author(s): 山口芳樹
  • Organizer: 第3回レクチン利用技術研究会
  • Invited
• [Presentation] Mammalian C-type lectin receptors: ligand recognition and signaling (2017)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: ACADEMIA Sinica symposium
  • Int'l Joint Research / Invited
• [Presentation] Cooperative ligand binding of a C-type lectin like receptor Dectin-1 (2017)
  • Author(s): Hari P. Dulal, Shinya Hanashima and Yoshiki Yamaguchi
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, 6th Annual Symposium
  • Int'l Joint Research
• [Presentation] 抗体-薬物複合体（ADC）の均一合成の試み： 現状の問題点と今後 (2017)
  • Author(s): 山口芳樹
  • Organizer: 44回BMSコンファレンス（BMS2017)
  • Invited
• [Presentation] 糖鎖改変を利用した抗体・薬物複合体の合成の試み (2017)
  • Author(s): 山口芳樹
  • Organizer: バイオ医療セミナー
  • Invited
• [Presentation] ENGaseを用いた抗体への糖鎖導入の条件検討 (2017)
  • Author(s): 山口芳樹
  • Organizer: 第3回ENGase研究会
• [Presentation] Dectin-1のβ-グルカン結合に伴う協同的なオリゴマー化の解析 (2017)
  • Author(s): デュラル ハリ、安達禎之、大野尚仁、山口芳樹
  • Organizer: 第36回 日本糖質学会年会
• [Presentation] Cooperative ligand binding of a C-type lectin like receptor Dectin-1 (2017)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: Glyco 24
  • Int'l Joint Research
• [Presentation] Looking for glycan structure code from a structural biology approach (2017)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: 江南大学セミナー
  • Invited
• [Presentation] Cooperative ligand binding of a C-type lectin like receptor Dectin-1 (2017)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: Society For Glycobiology, Portland
  • Int'l Joint Research
• [Presentation] 溶液NMR法によるtau凝集体と低分子化合物の相互作用解析 (2017)
  • Author(s): 山口芳樹、松本加奈、佐原成彦
  • Organizer: 第56回NMR討論会
• [Presentation] Structural Glycobiology: Glycan Structure, Dynamics and Interaction (2017)
  • Author(s): Yoshiki Yamaguchi
  • Organizer: Systems Glycobiology and Beyond
  • Int'l Joint Research
• [Presentation] ありふれた糖鎖がシグナルになる秘密： CLEC-2の立体構造から学ぶ (2017)
  • Author(s): 山口芳樹
  • Organizer: 第3回レクチン利用技術研究会
  • Invited
• [Presentation] Ion mobility spectrometry separation of isomeric N-glycan structures (2016)
  • Author(s): Yamaguchi, Y., Hirose, K., Nishima, W., Re, S., and Sugita, Y.
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, 5th Annual Symposium
  • Int'l Joint Research
• [Presentation] Understanding glycan dynamics and interaction by NMR and X-ray crystallography (2016)
  • Author(s): Yamaguchi, Y.
  • Organizer: 6th Charles Warren Workshop 2016
  • Int'l Joint Research / Invited
• [Presentation] Glycan Dynamics analyzed by combined NMR and X-ray crystallography (2016)
  • Author(s): Yamaguchi, Y.
  • Organizer: 8th Asian Community of Glycoscience and Glycotechnology, Wuxi, China, ACGG
  • Int'l Joint Research / Invited
• [Presentation] Mammalian C-type lectin receptors: ligand recognition and signaling (2016)
  • Author(s): Yamaguchi, Y.
  • Organizer: Third RIKEN-Academia Sinica Joint Conference
  • Int'l Joint Research / Invited
• [Presentation] An Approach to Detect a Metastable Minor Conformation of Branched N-glycans by Combined Analysis of Solution NMR and X-ray Crystallography (2016)
  • Author(s): Yamaguchi, Y.
  • Organizer: ICMRBS2016 International Conference on Magnetic Resonance in Biological Systems
  • Int'l Joint Research
• [Presentation] 分岐型糖鎖の非対称性とダイナミクス (2016)
  • Author(s): 山口芳樹
  • Organizer: 第35回 日本糖質学会年会
  • Invited
• [Presentation] レクチンを利用したN-結合型糖鎖の準安定構造の解析 (2016)
  • Author(s): 山口芳樹
  • Organizer: 第17回若手NMR研究会
• [Presentation] 生体内糖鎖認識の基盤と次世代セラノスティクス (2016)
  • Author(s): 山口芳樹、田中克典
  • Organizer: 第14回糖鎖科学コンソーシアムシンポジウム
  • Invited
• [Presentation] 予想もできなかった糖鎖認識の巧妙さ：バイセクト糖鎖結合レクチンを中心に (2016)
  • Author(s): 山口芳樹
  • Organizer: 第2回レクチン利用技術研究会
  • Invited
• [Book] βグルカンの基礎研究と応用・利用の動向 (2018)
  • Author(s): 山口芳樹
  • Total Pages: 280
  • Publisher: シーエムシー出版
  • ISBN: 9784781313405
• [Book] NMR in Glycoscience and Glycotechnology (2017)
  • Author(s): Yoshiki Yamaguchi, Hirokazu Yagi and Koichi Kato
  • Total Pages: 402
  • Publisher: Royal Society of Chemistry
  • ISBN: 9781782623106
• [Patent(Industrial Property Rights)] 認知症の診断マーカー及びそれを用いた認知症罹患鑑別方法 (2016)
  • Inventor(s): 橋本康弘、星京香、伊藤浩美、本多たかし、荒井啓行、古川勝敏他
  • Industrial Property Rights Holder: 橋本康弘、星京香、伊藤浩美、本多たかし、荒井啓行、古川勝敏他
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016