| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
CRISPR-Cas system serves as a prokaryotic adaptive immune system. In this study, I determined the crystal structures of Csm2 and Csm3, the Cas proteins that constitute the type III-A CRISPR-Cas effector complex. I constructed the model structure of the whole effector complex of the type III-A, and revealed the function of the Csm2 protein in the complex. Further, this study suggested the important role of Cmr1, one of the protein subunits of the type III-B CRISPR-Cas effector complex, in the 3’ terminal processing of the pre-crRNA of the type III effectors. To elucidate the structural and functional roles of Cmr1 in the 3’ terminal processing of the pre-crRNA, cryoEM analysis of the type III-B CRISPR-Cas effector complexes are currently in progress.
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