Structural and functional analysis of CRISPR-Cas effector complex
Project/Area Number |
16H04759
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Kyushu University (2019-2020) National Institute of Advanced Industrial Science and Technology (2016-2018) |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | CRISPR-Cas / crRNA / Casタンパク質 / エフェクター複合体 / 原核生物獲得免疫 / ゲノム / 核酸 / 立体構造解析 / III型CRISPR-Cas / Cmr複合体 / RNA-タンパク質複合体 / 遺伝子サイレンシング / 結晶構造解析 / Csm複合体 / CRISPR-Cas系 / 生体分子 / タンパク質-RNA複合体 |
Outline of Final Research Achievements |
CRISPR-Cas system serves as a prokaryotic adaptive immune system. In this study, I determined the crystal structures of Csm2 and Csm3, the Cas proteins that constitute the type III-A CRISPR-Cas effector complex. I constructed the model structure of the whole effector complex of the type III-A, and revealed the function of the Csm2 protein in the complex. Further, this study suggested the important role of Cmr1, one of the protein subunits of the type III-B CRISPR-Cas effector complex, in the 3’ terminal processing of the pre-crRNA of the type III effectors. To elucidate the structural and functional roles of Cmr1 in the 3’ terminal processing of the pre-crRNA, cryoEM analysis of the type III-B CRISPR-Cas effector complexes are currently in progress.
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Academic Significance and Societal Importance of the Research Achievements |
CRISPR-Cas系には多様なタンパク質とRNA分子が関与する。現在、ゲノム編集で利用されているのはクラス2のCas9とCas12である。一方、それ以外の因子は現在のところゲノム編集をはじめあまり活用されていない。さらに、機能が分かっていないCRISPR関連タンパク質も多数存在する。したがって、CRISPR-Cas系に関してさらに理解を深めることは、新規なゲノム編集技術の開発や新たな遺伝子発現調節技術の創出などに応用できる可能性を秘めている。本研究は、III型のエフェクター複合体に焦点を当てたものであり、新たな技術に結びつくことが期待できる研究成果である。
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Report
(5 results)
Research Products
(7 results)
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[Journal Article] Crystal structure and thermodynamic dissection of chitin oligosaccharide binding to the LysM module of chitinase-A from Pteris ryukyuensis2017
Author(s)
Ohnuma, T., Taira, T., Umemoto, N., Kitaoku, Y., Sørlie, M., Numata, T. and Fukamizo, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 494
Issue: 3-4
Pages: 736-741
DOI
Related Report
Peer Reviewed
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[Journal Article] Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase2016
Author(s)
Shinya, S., Nishimura, S., Kitaoku, Y., Numata, T., Kimoto, H., Kusaoke, H., Ohnuma, T. and Fukamizo, T.
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Journal Title
Biochem J.
Volume: 473
Issue: 8
Pages: 1085-1095
DOI
Related Report
Peer Reviewed
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