Development of novel materials by design of artificial proteins
| Project/Area Number |
16H04779
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
Tame Jeremy 横浜市立大学, 生命医科学研究科, 教授 (00336588)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
池上 貴久 横浜市立大学, 生命医科学研究科, 教授 (20283939)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
|
|---|
| Keywords | 人工たんぱく質 / 構造解析 / protein design / artificial protein / 人工タンパク質 / タンパク質設計 / X線構造解析 / ナノバイオテクノロジー / タンパク質構造解析 / 多量体 / ナノクラスター |
|---|
| Outline of Final Research Achievements |
Several novel proteins have been created, and tested for their ability to self-assemble from separate subunits. Various polyoxometalate (POM) clusters and symmetrical artificial proteins of matching symmetry have been combined to build large-scale hybrid assemblies with a protein called Pizza, that we designed and described previously. The POM-Pizza interaction was measured using various biophysical techniques, and crystal structures of the complexes were solved. In a separate project, a novel lectin from a species of mussel was found to bind to the glycan of asialo-GM1. The crystal structure of this protein has been solved, and further work to develop a useful biomedical tool from this protein, by building larger networks with it, is underway.
|
| Academic Significance and Societal Importance of the Research Achievements |
いくつかの癌細胞類は細胞外面にasialo-GM1という糖脂質を提示している。イガイの一種からみつかった新規レクチン(SEVIL)がasialo-GM1の糖鎖部分に結合することがわかった。 すでにSEVILの結晶構造解明に成功しており、このタンパク質から有用な癌検出ツールを開発するためのさらなる研究を進めている。SEVILの糖鎖に対する親和性は、比較的弱いため、SEVILが大きな多量体を形成するようなタンパク質の設計を試みている。これにより、非常に敏感な糖鎖検出ツールを創出できる。
|
Report
(4 results)
Research Products
(19 results)
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] (9)Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium2016
Author(s)
Ohki M, Sugiyama K, Kawai F, Tanaka H, Nihei Y, Unzai S, Takebe M, Matsunaga S, Adachi S, Shibayama N, Zhou Z, Koyama R, Ikegaya Y, Takahashi T, Tame J, Iseki M, Park S-Y.
-
Journal Title
Proc Natl Acad Sci USA
Volume: 113
Issue: 24
Pages: 6689-6664
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
-
-
