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Development of novel materials by design of artificial proteins

Research Project

Project/Area Number 16H04779
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionYokohama City University

Principal Investigator

Tame Jeremy  横浜市立大学, 生命医科学研究科, 教授 (00336588)

Co-Investigator(Kenkyū-buntansha) 池上 貴久  横浜市立大学, 生命医科学研究科, 教授 (20283939)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords人工たんぱく質 / 構造解析 / protein design / artificial protein / 人工タンパク質 / タンパク質設計 / X線構造解析 / ナノバイオテクノロジー / タンパク質構造解析 / 多量体 / ナノクラスター
Outline of Final Research Achievements

Several novel proteins have been created, and tested for their ability to self-assemble from separate subunits. Various polyoxometalate (POM) clusters and symmetrical artificial proteins of matching symmetry have been combined to build large-scale hybrid assemblies with a protein called Pizza, that we designed and described previously. The POM-Pizza interaction was measured using various biophysical techniques, and crystal structures of the complexes were solved. In a separate project, a novel lectin from a species of mussel was found to bind to the glycan of asialo-GM1. The crystal structure of this protein has been solved, and further work to develop a useful biomedical tool from this protein, by building larger networks with it, is underway.

Academic Significance and Societal Importance of the Research Achievements

いくつかの癌細胞類は細胞外面にasialo-GM1という糖脂質を提示している。イガイの一種からみつかった新規レクチン(SEVIL)がasialo-GM1の糖鎖部分に結合することがわかった。 すでにSEVILの結晶構造解明に成功しており、このタンパク質から有用な癌検出ツールを開発するためのさらなる研究を進めている。SEVILの糖鎖に対する親和性は、比較的弱いため、SEVILが大きな多量体を形成するようなタンパク質の設計を試みている。これにより、非常に敏感な糖鎖検出ツールを創出できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (19 results)

All 2019 2018 2017 2016

All Journal Article (12 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 12 results,  Open Access: 7 results,  Acknowledgement Compliant: 5 results) Presentation (6 results) (of which Int'l Joint Research: 6 results,  Invited: 6 results) Book (1 results)

  • [Journal Article] Computational design of symmetrical eight-bladed β-propeller proteins2019

    • Author(s)
      Noguchi Hiroki、Addy Christine、Simoncini David、Wouters Staf、Mylemans Bram、Van Meervelt Luc、Schiex Thomas、Zhang Kam Y. J.、Tame Jeremy R. H.、Voet Arnout R. D.
    • Journal Title

      IUCrJ

      Volume: 6 Issue: 1 Pages: 46-55

    • DOI

      10.1107/s205225251801480x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular basis of hemoglobin adaptation in the high-flying bar-headed goose2018

    • Author(s)
      Natarajan Chandrasekhar、Jendroszek Agnieszka、Kumar Amit、Weber Roy E.、Tame Jeremy R. H.、Fago Angela、Storz Jay F.
    • Journal Title

      PLOS Genetics

      Volume: 14 Issue: 4 Pages: e1007331-e1007331

    • DOI

      10.1371/journal.pgen.1007331

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The crystal structure and oligomeric form of Escherichia coli l , d -carboxypeptidase A2018

    • Author(s)
      Meyer Karen、Addy Christine、Akashi Satoko、Roper David I.、Tame Jeremy R.H.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 499 Issue: 3 Pages: 594-599

    • DOI

      10.1016/j.bbrc.2018.03.195

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies2018

    • Author(s)
      Noguchi Hiroki、Mylemans Bram、De Zitter Elke、Van Meervelt Luc、Tame Jeremy R.H.、Voet Arnout
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 497 Issue: 4 Pages: 1038-1042

    • DOI

      10.1016/j.bbrc.2018.02.168

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Seeing the light with BLUF proteins2017

    • Author(s)
      Sam-Yong Park and Jeremy R. H. Tame
    • Journal Title

      Biophysical Reviews

      Volume: 9 Issue: 2 Pages: 169-176

    • DOI

      10.1007/s12551-017-0258-6

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular mechanism of photoactivation of a light-regulated adenylate cyclase.2017

    • Author(s)
      Ohki M, Sato-Tomita A, Matsunaga S, Iseki M, Tame JRH, Shibayama N, Park SY.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 114 Pages: 8562-8567

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Direct observation of conformational population shifts in crystalline human hemoglobin.2017

    • Author(s)
      Shibayama N, Ohki M, Tame JRH, Park SY.
    • Journal Title

      J Biol Chem.

      Volume: 292 Pages: 18258-18269

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Computational design of a symmetrical β-trefoil lectin with cancer cell binding activity2017

    • Author(s)
      Daiki Terada , Arnout R. D. Voet, Hiroki Noguchi, Kenichi Kamata, Mio Ohki, Christine Addy, Yuki Fujii, Daiki Yamamoto, Yasuhiro Ozeki , Jeremy R. H. Tame & Kam Y. J. Zhang
    • Journal Title

      Scientific REPOrTs

      Volume: 7 Issue: 1 Pages: 5943-5943

    • DOI

      10.1038/s41598-017-06332-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Protein-templated synthesis of metal-based nanomaterials.2017

    • Author(s)
      Voet AR, Tame JRH
    • Journal Title

      Curr Opin Biotechnol

      Volume: 46 Pages: 14-19

    • DOI

      10.1016/j.copbio.2016.10.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evolution-Inspired Computational Design of Symmetric Proteins.2017

    • Author(s)
      Voet AR, Simoncini D, Tame JRH, Zhang KY.
    • Journal Title

      Methods Mol Biol

      Volume: 1529 Pages: 309-322

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types.2016

    • Author(s)
      Terada D, Kawai F, Noguchi H, Unzai S, Hasan I, Fujii Y, Park SY, Ozeki Y, Tame JRH
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 28344-28344

    • DOI

      10.1038/srep28344

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] (9)Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium2016

    • Author(s)
      Ohki M, Sugiyama K, Kawai F, Tanaka H, Nihei Y, Unzai S, Takebe M, Matsunaga S, Adachi S, Shibayama N, Zhou Z, Koyama R, Ikegaya Y, Takahashi T, Tame J, Iseki M, Park S-Y.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 113 Issue: 24 Pages: 6689-6664

    • DOI

      10.1073/pnas.1517520113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Protein design for new frameworks and networks2019

    • Author(s)
      Jeremy Tame
    • Organizer
      The Second International workshop by the 174th committee JSPS on Symbiosis of Biology and Nanodevices
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Photoactivation of a light-regulated adenylate cyclase2018

    • Author(s)
      Jeremy Tame
    • Organizer
      The Astbury Conversation UK
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A vision of the future2018

    • Author(s)
      Jeremy Tame
    • Organizer
      BIO NANO 2018 Krakow Poland
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Photoactivation of a light-regulated adenylate cyclase2018

    • Author(s)
      Tame JRH
    • Organizer
      Leeds University, UK Astbury Conversation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Seeing the light with BLUF proteins2017

    • Author(s)
      Jeremy Tame
    • Organizer
      JCUP VIII
    • Place of Presentation
      大手町サンスカイルーム(東京都千代田区)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Aspects of Protein Design2017

    • Author(s)
      Tame JRH
    • Organizer
      Symposium  KU University
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Approaches to Entropy2019

    • Author(s)
      Jeremy R. H. Tame
    • Total Pages
      202
    • Publisher
      Springer
    • ISBN
      9789811323157
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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