| Project/Area Number |
16H04779
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Yokohama City University |
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Principal Investigator |
Tame Jeremy 横浜市立大学, 生命医科学研究科, 教授 (00336588)
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| Co-Investigator(Kenkyū-buntansha) |
池上 貴久 横浜市立大学, 生命医科学研究科, 教授 (20283939)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Keywords | 人工たんぱく質 / 構造解析 / protein design / artificial protein / 人工タンパク質 / タンパク質設計 / X線構造解析 / ナノバイオテクノロジー / タンパク質構造解析 / 多量体 / ナノクラスター |
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| Outline of Final Research Achievements |
Several novel proteins have been created, and tested for their ability to self-assemble from separate subunits. Various polyoxometalate (POM) clusters and symmetrical artificial proteins of matching symmetry have been combined to build large-scale hybrid assemblies with a protein called Pizza, that we designed and described previously. The POM-Pizza interaction was measured using various biophysical techniques, and crystal structures of the complexes were solved. In a separate project, a novel lectin from a species of mussel was found to bind to the glycan of asialo-GM1. The crystal structure of this protein has been solved, and further work to develop a useful biomedical tool from this protein, by building larger networks with it, is underway.
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| Academic Significance and Societal Importance of the Research Achievements |
いくつかの癌細胞類は細胞外面にasialo-GM1という糖脂質を提示している。イガイの一種からみつかった新規レクチン(SEVIL)がasialo-GM1の糖鎖部分に結合することがわかった。 すでにSEVILの結晶構造解明に成功しており、このタンパク質から有用な癌検出ツールを開発するためのさらなる研究を進めている。SEVILの糖鎖に対する親和性は、比較的弱いため、SEVILが大きな多量体を形成するようなタンパク質の設計を試みている。これにより、非常に敏感な糖鎖検出ツールを創出できる。
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