The molecular sysytem determining differene among regnerative abilities in species
Project/Area Number |
16H04794
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | Tottori University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
荻野 肇 広島大学, 両生類研究センター, 教授 (10273856)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | 再生現象 / 心臓 / 肝臓 / イベリアトゲイモリ / マウス / イモリ / 心臓肝臓 イモリマウス / 肝臓 イモリマウス / イモリマウス / 細胞周期 / 器官形成 / 器官再生 / 遺伝子発現制御 |
Outline of Final Research Achievements |
We found that cyclin D1, the first cell cycle regulator, is expressed in the mouse liver and newt heart which can regenerate , but not in mouse heart which can not regenerate, suggesting that the expression determines the difference of regenerative abilities. We also produced transgenic mice and newts by which expression of cycin D1 can be monitored. A comprehensive transcriptome resource for Iberian ribbed newt was established. In addition, we showed that the newt genes can be efficiently disrupted by CRISPR/cas 9 system. These results enable knockout experiments for given genes, and enhance future research employing newts as a model animal for regeneration research as well as for investigations in other areas.
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Academic Significance and Societal Importance of the Research Achievements |
生物が種や組織によって再生できる、できないの違いの分子機構を知ることは再生現象のしくみを理解するため、また、私たちヒトで再生できない組織を再生できる再生医療への発展に重要である。本研究の成果では、イモリとマウス、肝臓と心臓と種や組織が異なっていても共通してサイクリンD1の発現の有無が増殖を伴う再生能に関わることを示した。この結果は再生の機構として損傷後にこの遺伝子を発現させる機構が必ずあることを示している。また、ヒトの再生医療としてこれまで再生できない組織を再生させるためにはこの遺伝子と下流の細胞周期進行因子の安定した発現や活性化を適切に制御することで実現できる可能性を示した。
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Report
(4 results)
Research Products
(81 results)
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[Journal Article] Comparative analysis demonstrates cell type-specific conservation of SOX9 targets between mouse and chicken.2019
Author(s)
Yamashita S, Kataoka K, Yamamoto H, Kato T, Hara S, Yamaguchi K, Renard-Guillet C, Katou Y, Shirahige K, Ochi H, Ogino H, Uchida T, Inui M, Takada S, Shigenobu S, and Asahara H
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 12560-12560
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Draft genome of Dugesia japonica provides insights into conserved regulatory elements of the brain restriction gene nou-darake in planarians.2018
Author(s)
5.An, Y., Kawaguchi, A., Zhao, C., Toyoda, A., Sharifi-Zarchi, A., Mousavi, S. A., Bagherzadeh, R., Inoue, T., Ogino, H., Fujiyama, A., Chitsaz, H., Baharvand, H. and Agata, K.
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Journal Title
Zoological Lett.
Volume: 4
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Conservatism and variability of gene expression profiles among homeologous transcription factors in Xenopus laevis.2017
Author(s)
Watanabe, M., Yasuoka, Y., Mawaribuchi, S., Kuretani, A., Ito, M., Kondo, M., Ochi, H., Ogino, H., Fukui, A., Taira, M. and Kinoshita, T.
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Journal Title
Dev. Biol.
Volume: 426
Issue: 2
Pages: 301-324
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] The functional domain-localized mutations hidden in the allotetraploid genome of Xenopus laevis.2019
Author(s)
Tanouchi, M., Iwata, Y., Igawa, T., Sakagami, K., Suzuki, N. and Ogino, H.
Organizer
第52回日本発生生物学会年会
Related Report
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[Presentation] The functional domain-localized mutations hidden in the allotetraploid genome of Xenopus laevis2018
Author(s)
Tanouchi, M., Iwata, Y., Igawa, T., Sakagami, K., Suzuki, N. and Ogino, H.
Organizer
第41回日本分子生物学会年会
Related Report
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[Presentation] Geneolgy and pedigrees of inbreeding strains of Xenopus tropicalis2018
Author(s)
Igawa, T., Kashiwagi, A., Kashiwagi, K., Suzuki, N., Watanabe, A., Suzuki, A., Noble, A., Guille, M., Simpson, D. E., Horb, M. E., Fujii, T., Sumida, M. and Ogino, H.
Organizer
17th International Xenopus Conference
Related Report
Int'l Joint Research
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[Presentation] The hypomorphic mutations hidden in the allotetraploid genome of Xenopus laevis.2018
Author(s)
Tanouchi, M., Ochi, H., Kawaguchi, A., Igawa, T., Iwata, Y., Sakagami, K. and Ogino, H.
Organizer
第70回日本細胞生物学会・第51回日本発生生物学会合同大会
Related Report
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[Presentation] The wild-type Xenopus laevis is an asymptomatic carrier of aniridia-like pax6 mutations.2018
Author(s)
Iwata, Y., Tanouchi, M., Igawa, T., Sakagami, K., Ochi, H. and Ogino, H.
Organizer
第70回日本細胞生物学会・第51回日本発生生物学会合同大会
Related Report
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[Presentation] Modification of cell differentiation competence by histone H3K27 demethylases and their involvement in tissue regeneration in Xenopus2016
Author(s)
Ogino, H., Sudou, N., Kawaguchi, A., Ochi, H., Kamiguchi, S. and Kasahara, H.
Organizer
ICZ/ZSJ Joint Meeting Symposium
Place of Presentation
OIST (Onna-son, Okinawa)
Year and Date
2016-11-15
Related Report
Invited
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