• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genome-morphological elucidation of structure and function of intracellular 3 organelle division machinery

Research Project

Project/Area Number 16H04813
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionJapan Women's University

Principal Investigator

KUROIWA Tsuneyoshi  日本女子大学, 理学研究科, 客員教授 (50033353)

Co-Investigator(Kenkyū-buntansha) 永田 典子  日本女子大学, 理学部, 教授 (40311352)
Research Collaborator KUROIWA haruko  
IMOTO yuuta  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords葉緑体分裂マシン / ミトコンドリア分裂マシン / ペルオキシソーム分裂マシン / シゾンゲノム / 細胞小器官 / 葉緑体分裂リング / ミトコンドリア分裂リング / ペルオキシソーム分裂リング / オルガネラ分裂マシン / ゲノム形態学 / 細胞小器官(オルガネラ) / 細胞・組織 / オルガネラ / 分裂マシン / 超微細構造 / ゲノム形態 / PD/MDリング / PODリング / ダイナミンリング
Outline of Final Research Achievements

In addition to cell nuclei, eukaryotic cells are maintained in vital activity by six types of organelles such as mitochondria that divide / proliferate with the cell nucleus. The applicants have so far grasped that mitochondria, chloroplast and peroxisomes divide / proliferate by contraction of the dividing machinery: MD ring, PD ring and POD ring. Therefore, the purpose of this research was to know the composition of each ring, elucidate the commonality, and consider the evolutionary significance. As a result, we clarified that PD and MD rings are a bundle of glucose fibers, and that MD and POD rings function to contract and divide by a common enzyme (dynamo 1). Furthermore, the similarity between MD and PD suggested that both organelles were born from a common ancestor.

Academic Significance and Societal Importance of the Research Achievements

真核細胞は細胞核の他に6種の細胞小器官が生命活動を支えている。ミトコンドリアはエネルギー源ATPを生み出すが、その際に大量のCO2や猛毒の活性酸素を発生させる。これらを無毒化するのがぺルオキシソームである。ミトコンドリア病や癌、ペルオキシソーム病などには、これら細胞小器官の細胞内数が関与している。本研究成果はこうした医療にも応用されよう。一方植物は、葉緑体が光合成によりCO2を固定し温暖化を防ぐと共に、O2と糖(食料)を生み出し、地球環境の維持や生物の生存を支えている。医療にも、植物の生産性の向上にも、分裂マシンの基本機能の解明は必須であり、これを使った応用研究の貢献は大きい。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (24 results)

All 2018 2017 2016

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 9 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomes2018

    • Author(s)
      Imoto Yuuta、Abe Yuichi、Honsho Masanori、Okumoto Kanji、Ohnuma Mio、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Fujiki Yukio
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41467-018-07009-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Holliday junction resolvases mediate chloroplast nucleoid segregation2017

    • Author(s)
      Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y.
    • Journal Title

      Science

      Volume: 356 Issue: 6338 Pages: 631-634

    • DOI

      10.1126/science.aan0038

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017

    • Author(s)
      Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 114 Issue: 50 Pages: 13284-13289

    • DOI

      10.1073/pnas.1715008114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Defining dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae.2017

    • Author(s)
      Imoto, Y., Abe, Y., Okumoto, K., Honsho, M., Kuroiwa, H., Kuroiwa, T., and *Fujiki, Y.
    • Journal Title

      J. Cell Sci.

      Volume: 130 Issue: 5 Pages: 853-867

    • DOI

      10.1242/jcs.199182

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017

    • Author(s)
      Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
    • Journal Title

      Front. Plant Sci.

      Volume: 8 Pages: 343-343

    • DOI

      10.3389/fpls.2017.00343

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] シゾンとメダカモから探る真核生物の増殖の基本原理2017

    • Author(s)
      黒岩常祥
    • Journal Title

      Plant Morophology

      Volume: 29 Pages: 63-71

    • NAID

      130006647165

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Surfing the vegetal pole in a small population: extracellular vertical transmission of an 'intracellular' deep-sea clam symbiont2016

    • Author(s)
      Tetsuro Ikuta, Kanae Igawa, Akihiro Tame, Tsuneyoshi Kuroiwa, Haruko Kuroiwa, Yui Aoki, Yoshihiro Takaki, Yukiko Nagai, Genki Ozawa, Masahiro Yamamoto, Ryusaku Deguchi, Katsunori Fujikura, Tadashi Maruyama, Takao Yoshida
    • Journal Title

      Royal Society Open Science

      Volume: 3 Issue: 5 Pages: 160130-160130

    • DOI

      10.1098/rsos.160130

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i>2016

    • Author(s)
      Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H.
    • Journal Title

      CYTOLOGIA

      Volume: 81 Issue: 1 Pages: 69-76

    • DOI

      10.1508/cytologia.81.69

    • NAID

      130005143755

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Intracellular Structure of the Unicellular Red Alga <i>Cyanidioschyzon merolae</i> in Response to Phosphate Depletion and Resupplementation2016

    • Author(s)
      Yagisawa, F., Kuroiwa, H.,Fujiwara, T. and Kuroiwa, T.
    • Journal Title

      CYTOLOGIA

      Volume: 81 Issue: 3 Pages: 341-347

    • DOI

      10.1508/cytologia.81.341

    • NAID

      130005265019

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 真核細胞の基本構造と機能をシゾンとメダカモの比較ゲノム形態から解く(2)2018

    • Author(s)
      黒岩常祥、黒岩晴子、永田典子、本多珠巳、田草川真理、三角修己、加藤翔一、乾弥生、松永朋子、松永幸大
    • Organizer
      日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ミトコンドリア分裂リング合成遺伝子MDR1の同定と機能の解析.2018

    • Author(s)
      吉田大和、黒岩晴子、嶋田崇史、吉田昌樹、大沼みお、藤原崇之、井元祐太、八木沢芙美、西田啓二、廣岡俊亮、三角修己、茂木祐子、赤壁善彦、松下一信、黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 緑藻メダカモのゲノム解析2017

    • Author(s)
      加藤翔一、松永幸大、三角修己、乾弥生、黒岩晴子、黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] フウロソウ科植物の胚嚢細胞内オルガネラ のダイナミクスについて2017

    • Author(s)
      黒岩晴子、永田典子、黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeの細胞分裂における ESCRTの役割2017

    • Author(s)
      八木沢芙美、藤原崇之、宮城島進也、中村宗一 黒岩晴子、黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シゾンのオーロラキナーゼによるミトコンドリア分裂制御機構の解析2017

    • Author(s)
      松永朋子、加藤翔一、岡村枝里佳、坂本卓也、乾弥生、黒岩常祥、松永幸大
    • Organizer
      日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 真核細胞の基本構造と機能をシゾンとメダカモのゲノム形態から解く2017

    • Author(s)
      黒岩常祥、黒岩晴子、永田典子、田草川真理、三角修己、加藤翔一、乾弥生、 松永朋子、松永幸大
    • Organizer
      日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シゾンとメダカモから探る真核生物の増殖の基本原理2016

    • Author(s)
      黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 単細胞藻類シゾンを用いたオーロラキナーゼによるミトコンドリア分裂制御メカニズムの解明2016

    • Author(s)
      岡村枝里佳 松永朋子 坂本卓也 黒岩常祥 松永幸大
    • Organizer
      日本植物学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ペルオキシソーム分裂装置の構造と機能解析2016

    • Author(s)
      井元祐太 阿部雄一 奥本寛治 本庄雅則 黒岩晴子 黒岩常祥 藤木幸夫
    • Organizer
      日本植物学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 紅藻シゾンと極小緑藻メダカモから真核細胞の誕生・増殖の基本原理を探る2016

    • Author(s)
      黒岩常祥 黒岩晴子 永田典子 三角修己 田草川真理 井元祐太 八木沢芙美 乾弥生 松永幸大
    • Organizer
      日本植物学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 藻類における脂質合成に関わる細胞小器官の機能について2016

    • Author(s)
      黒岩晴子 三角修己 井元祐太 大沼みお 黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolae の構成的プラスミドと抗生物質耐性マーカーの開発2016

    • Author(s)
      大沼みお 藤原崇之 井元祐太 黒岩晴子 宮城島進也 田中寛 黒岩常祥
    • Organizer
      日本植物学会
    • Related Report
      2016 Annual Research Report
  • [Book] Cyanidioschyzon merolae : A NewModel Eukaryote for Cell and Organelle Biology2018

    • Author(s)
      ①Kuroiwa, T.,Miyagishima, S.,Matsunaga, S., Sato, N.,Nozaki, H., Tanaka, K., Misumi, O.
    • Total Pages
      365
    • Publisher
      Springer
    • ISBN
      9789811061004
    • Related Report
      2018 Annual Research Report
  • [Book] Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology2018

    • Author(s)
      Tsuneyoshi Kuroiwa, Sinya Miyagishima, Sachihiro Matsunaga, Naoki Sato, Hisayoshi Nozaki, Kan Tanaka, Osami Misumi Editors
    • Total Pages
      365
    • Publisher
      Springer
    • ISBN
      9789811061004
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi