Genome-morphological elucidation of structure and function of intracellular 3 organelle division machinery
| Project/Area Number |
16H04813
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Japan Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永田 典子 日本女子大学, 理学部, 教授 (40311352)
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| Research Collaborator |
KUROIWA haruko
IMOTO yuuta
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Keywords | 葉緑体分裂マシン / ミトコンドリア分裂マシン / ペルオキシソーム分裂マシン / シゾンゲノム / 細胞小器官 / 葉緑体分裂リング / ミトコンドリア分裂リング / ペルオキシソーム分裂リング / オルガネラ分裂マシン / ゲノム形態学 / 細胞小器官（オルガネラ） / 細胞・組織 / オルガネラ / 分裂マシン / 超微細構造 / ゲノム形態 / PD/MDリング / PODリング / ダイナミンリング |
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| Outline of Final Research Achievements |
In addition to cell nuclei, eukaryotic cells are maintained in vital activity by six types of organelles such as mitochondria that divide / proliferate with the cell nucleus. The applicants have so far grasped that mitochondria, chloroplast and peroxisomes divide / proliferate by contraction of the dividing machinery: MD ring, PD ring and POD ring. Therefore, the purpose of this research was to know the composition of each ring, elucidate the commonality, and consider the evolutionary significance. As a result, we clarified that PD and MD rings are a bundle of glucose fibers, and that MD and POD rings function to contract and divide by a common enzyme (dynamo 1). Furthermore, the similarity between MD and PD suggested that both organelles were born from a common ancestor.
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| Academic Significance and Societal Importance of the Research Achievements |
真核細胞は細胞核の他に6種の細胞小器官が生命活動を支えている。ミトコンドリアはエネルギー源ATPを生み出すが、その際に大量のCO2や猛毒の活性酸素を発生させる。これらを無毒化するのがぺルオキシソームである。ミトコンドリア病や癌、ペルオキシソーム病などには、これら細胞小器官の細胞内数が関与している。本研究成果はこうした医療にも応用されよう。一方植物は、葉緑体が光合成によりCO2を固定し温暖化を防ぐと共に、O2と糖（食料）を生み出し、地球環境の維持や生物の生存を支えている。医療にも、植物の生産性の向上にも、分裂マシンの基本機能の解明は必須であり、これを使った応用研究の貢献は大きい。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Holliday junction resolvases mediate chloroplast nucleoid segregation2017
Author(s)
Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y.
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Journal Title
Science
Volume: 356
Issue: 6338
Pages: 631-634
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017
Author(s)
Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 114
Issue: 50
Pages: 13284-13289
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Defining dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae.2017
Author(s)
Imoto, Y., Abe, Y., Okumoto, K., Honsho, M., Kuroiwa, H., Kuroiwa, T., and *Fujiki, Y.
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Journal Title
J. Cell Sci.
Volume: 130
Issue: 5
Pages: 853-867
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017
Author(s)
Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
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Journal Title
Front. Plant Sci.
Volume: 8
Pages: 343-343
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Surfing the vegetal pole in a small population: extracellular vertical transmission of an 'intracellular' deep-sea clam symbiont2016
Author(s)
Tetsuro Ikuta, Kanae Igawa, Akihiro Tame, Tsuneyoshi Kuroiwa, Haruko Kuroiwa, Yui Aoki, Yoshihiro Takaki, Yukiko Nagai, Genki Ozawa, Masahiro Yamamoto, Ryusaku Deguchi, Katsunori Fujikura, Tadashi Maruyama, Takao Yoshida
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Journal Title
Royal Society Open Science
Volume: 3
Issue: 5
Pages: 160130-160130
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i>2016
Author(s)
Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., Kuroiwa, H.
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Journal Title
CYTOLOGIA
Volume: 81
Issue: 1
Pages: 69-76
DOI
NAID
ISSN
0011-4545, 1348-7019
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Book] Cyanidioschyzon merolae : A NewModel Eukaryote for Cell and Organelle Biology2018
Author(s)
①Kuroiwa, T.,Miyagishima, S.,Matsunaga, S., Sato, N.,Nozaki, H., Tanaka, K., Misumi, O.
Total Pages
365
Publisher
Springer
ISBN
9789811061004
Related Report
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