Study on the residual green alga-derived nucleomorphs in dinoflagellates
| Project/Area Number |
16H04826
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Inagaki Yuji 筑波大学, 計算科学研究センター, 教授 (50387958)
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| Co-Investigator(Kenkyū-buntansha) |
谷藤 吾朗 独立行政法人国立科学博物館, 動物研究部, 研究員 (70438480)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 細胞内共生 / 二次共生 / ヌクレオモルフ / ゲノム縮退 / 渦鞭毛藻 |
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| Outline of Final Research Achievements |
Nucleomorphs (NMs) are relic endosymbiont nuclei so far found only in two algal groups, cryptophytes and chlorarachniophytes, which have been studied extensively to model the evolutionary process integrating an endosymbiont alga into be a host-governed plastid (organellogenesis). To deepen our knowledge on organellogenesis, we need novel NM-bearing algae, we here investigated the genome and transcriptome data of two previously undescribed dinoflagellates, strains MGD and TGD, with green alga-derived plastids as well as the NMs. Our detailed analyses revealed that the overall trend in gene transfer from the NM to host genome is common among the two dinoflagellates investigated in this study, chlorarachniophytes and cryptophytes. Furthermore, DNA transfer between the host and endosymbiont nuclei was found to be in progress in both MGD and TGD systems.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞内共生体が葉緑体として確立する過程は、共生藻の痕跡核（NM)をもつクロララクニオン藻とクリプト藻をモデルとして研究されてきた。しかし、上記2系統では、葉緑体化に必要な共生藻－宿主間での遺伝子転移（EGT）は完了している。従って、葉緑体獲得過程をより深く理解するためにはNMをもつ新たな系統が必要であり、本研究ではNMをもつ未記載渦鞭毛藻2種のゲノム・トランスクリプトーム解析を行った。本研究では渦鞭毛藻2種ではEGTが未完了であり、葉緑体獲得過程を研究する上で新たなモデルとなるうることを明らかにした。今後渦鞭毛藻2種の研究により、葉緑体獲得過程の理解がより進展すると期待できる。
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Fates of Evolutionarily Distinct, Plastid- type Glyceraldehyde 3-phosphate Dehydrogenase Genes in Kareniacean Dinoflagellates2018
Author(s)
Kamikawa, R., Yazaki, E., Tahara, M., Sakura, T., Matsuo, E., Nagamune, K., Hashimoto, T., Inagaki, Y.
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Journal Title
J. Eukaryot. Microbiol.
Volume: 印刷中
Issue: 5
Pages: 669-678
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase2018
Author(s)
Zhonova Kristina, Petrzelkova Romana, Valach Matus, Yazaki Euki, Tikhonenkov Denis V., Butenko Anzhelika, Janouskovec Jan, Hrda Stepanka, Klimes Vladimir, Gurger Gertraud, Inagaki Yuji, Keeling Patrick J., Hampl Vladimir, Flegontov Pavel, Yurchenko Vyacheslav, Elias Marek.
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 5239-5239
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Global Kinetoplastea phylogeny inferred from a large-scale multigene alignment including parasitic species for better understanding transitions from a free-living to a parasitic lifestyle2017
Author(s)
Yazaki, E., Ishikawa, Sohta. A., Kume, K., Kumagai, A., Kamaishi, T., Tanifuji, G., Hashimoto, T., and Inagaki, Y.
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Journal Title
Genes & Genetic Systems
Volume: 92
Issue: 1
Pages: 35-42
DOI
NAID
ISSN
1341-7568, 1880-5779
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Organelles that illuminate the origins of Trichomonas hydrogenosomes and Giardia mitosomes2017
Author(s)
Leger M, Kolisko M, Ryoma Kamikawa, Stairs CW, Kume K, Cepicka I, Silberman JD, Andersson JO, Xu F, Yabuki A, Zhang Q, Takishita K, Inagaki Y, Simpson AGB, Hashimoto T, Roger AJ
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Journal Title
Nature Ecology and Evolution
Volume: 1
Issue: 4
Pages: 0092-0092
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] A Non-photosynthetic Diatom Reveals Early Steps of Reductive Evolution in Plastids2017
Author(s)
Kamikawa R, Moog D, Zauner S, Tanifuji G, Ishida K, Miyashita H, Mayama S, Hashimoto T, Maier UG, Archibald JM, Inagaki Y
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Journal Title
Molecular Biology and Evolution
Volume: 34
Issue: 9
Pages: 2355-2366
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ungulate malaria parasites2016
Author(s)
Templeton TJ, Asada M, Jiratanh M, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea S, Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 23230-23230
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Orphan protistology，accelerating in Japan2018
Author(s)
Takashi Shiratori，Akinori Yabuki，Euki Yazaki，Yuki Nishimura，Moriya Ohkuma，Katsunori Fujikura，Tetsuo Hashimoto，Yuji Inagaki，Ken-ichiro Ishida
Organizer
Joint meeting of the Japan Society of Protistology and the Korean Society of Protozoologists
Related Report
Int'l Joint Research
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[Presentation] Molecular tinkering in the evolution of the membrane attachment mechanisms of the Rheb GTPase2017
Author(s)
Zhonova Kristina, Petrzelkova Romana, Yazaki Euki, Nishimura Yuki, Valach Matus, Hrda Stepanka, Klimes Vladimir, Inagaki Yuji, Burger Gertraud, Hampl Vladimir, Yurchenko Vyacheslav, Elias Marek.
Organizer
ICOP2017
Related Report
Int'l Joint Research
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[Presentation] Ungulate malaria parasites2016
Author(s)
Templeton Thomas J.、Asada Masahito、Jiratanh Montakan、Ishikawa Sohta A.、Tiawsirisup Sonthaya、Sivakumar Thillaiampalam、Namangala Boniface、Takeda Mika、Mohkaew Kingdao、Ngamjituea Supawan、Inoue Noboru、Sugimoto Chihiro、Inagaki Yuji、Suzuki Yasuhiko、Yokoyama Naoaki、Kaewthamasorn Morakot、Kaneko Osamu
Organizer
15th Awaji International Forum on Infection and Immunity
Related Report
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[Presentation] Loss of the Calvin Benson cycle in non-photosynthetic plastids2016
Author(s)
Ryoma Kamikawa, Stefan Zauner, Daniel Moog, Goro Tanifuji, Ken-ichiro Ishida, Shigeki Mayama, Tetsuo Hashimoto, John M Archibald, Andrew J Roger, Uwe-G Maier, Hideaki Miyashita, Yuji Inagaki
Organizer
13th International Colloquium on Endocytobiology and Symbiosis
Related Report
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