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Genomics-based breeding strategy to develop Ceratocystis canker resistance lines in fig (Ficus carica)

Research Project

Project/Area Number 16H04878
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionKazusa DNA Research Institute

Principal Investigator

Shirasawa Kenta  公益財団法人かずさDNA研究所, 先端研究開発部, 主任研究員 (60527026)

Co-Investigator(Kenkyū-buntansha) 薬師寺 博  国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, ユニット長 (00355364)
池上 秀利  福岡県農林業総合試験場, 豊前分場, 研究員 (40502414)
Research Collaborator Morita Takeshige  広島県立総合技術研究所, 農業技術センター
Jikumaru Shota  広島県立総合技術研究所, 農業技術センター
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsイチジク / 抵抗性 / 雌雄性
Outline of Final Research Achievements

We established an efficient breeding system for Ceratocystis canker resistance lines in fig (Ficus carica). In this study, we determined high-quality genome sequences of fig and F. erecta which is a donor plant of the resistance, constructed a high-density genetic map based on single nucleotide polymorphism markers, and identified genetic loci for the resistance and sex determinant. As a result, we developed DNA markers linked to the loci to select resistance and female lines, which able to set bear fruits, in fig breeding programs. Through this study, we found out candidate genes for the resistance and sex determinant from the genome information.

Academic Significance and Societal Importance of the Research Achievements

本研究では、近年のイチジク栽培において多大な被害をもたらしている株枯病に対して真性抵抗性を示すイチジクを効率的に育種するための技術開発を行った。先端ゲノム解析技術を利用して、イチジク近縁種のイヌビワが持つ高度株枯病真性抵抗性遺伝子座を短期間、かつ、低コストにイチジクに導入できる技術を確立した。さらに、イチジク属が持つ雌雄性決定遺伝子座を明らかにし、食用価値のある雌性イチジクを幼苗段階で選抜できる方法を開発した。これらの成果により、株枯病真性抵抗性遺伝子を有する雌性イチジク品種が高効率に開発できるようになると期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (3 results)

  • [Journal Article] Ceratocystis canker resistance in BC1 populations of interspecific hybridization of fig (Ficus carica) and F. erecta2019

    • Author(s)
      Yakushiji Hiroshi、Morita Takeshige、Jikumaru Shota
    • Journal Title

      Scientia Horticulturae

      Volume: 252 Pages: 71-76

    • DOI

      10.1016/j.scienta.2019.03.039

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.).2017

    • Author(s)
      Mori K, Shirasawa K, Nogata H, Hirata C, Tashiro K, Habu T, Kim S, Himeno S, Kuhara S, Ikegami H
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 41124-41124

    • DOI

      10.1038/srep41124

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] イチジクRAN1遺伝子上のSNPを検出するDNAマーカーの開発と迅速PCRによる雌雄判別2019

    • Author(s)
      池上秀利・白澤健太・薬師寺博・姫野修一
    • Organizer
      平成31年度園芸学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of RAN1 Orthologue Associated with Sex Determination in Gynodioecious Fig (Ficus carica L.)2018

    • Author(s)
      Kazuki Mori, Kenta Shirasawa, Hitoshi Nogata, Chiharu Hirata, Kosuke Tashiro, Tsuyoshi Habu, Sangwan Kim, Shuichi Himeno, Satoru Kuhara and Hidetoshi Ikegami
    • Organizer
      Plant & Animal Genome Conference XXVI
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] イチジクとイヌビワの種間交雑体 「BC1」が有するイチジク株枯病に対する抵抗性の評価2018

    • Author(s)
      森田剛成・軸丸祥大・薬師寺博
    • Organizer
      日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イチジクの性表現型と関連するRAN1の同定と雌雄判別マーカーの開発.2017

    • Author(s)
      白澤健太、森 一樹、野方 仁、平田千春、田代康介、羽生 剛、金 相完、姫野修一、久原 哲、池上秀利
    • Organizer
      園芸学会
    • Place of Presentation
      日本大学生物資源科学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genomic Information for High-Throughput Fruit Crop Breeding2017

    • Author(s)
      Kenta Shirasawa, Hidetoshi Ikegami, Kanji Isuzugawa, Mitsunobu Ikenaga, Hideki Hirakawa and Sachiko Isobe
    • Organizer
      Plant and Animal Genome Conference ASIA 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] イチジクとイヌビワの種間雑種由来のイチジク株枯れ抵抗性BC1の獲得.2016

    • Author(s)
      薬師寺博、森田剛成、軸丸祥大
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学天白キャンパス
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Remarks] Ficus Genome DataBase

    • URL

      http://ficus.kazusa.or.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 世界で初めてイチジクのゲノム配列の解読に成功~品種開発の加速に期待~

    • URL

      http://www.kazusa.or.jp/j/information/re_info/2017/0127.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 世界で初めてイチジクのゲノム配列の解読に成功

    • URL

      http://www.pref.fukuoka.lg.jp/press-release/itizikugenomu.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-01-27  

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