Molecular mechanism of nicotianamine secretion and its role in plants.

• Project/Area Number: 16H04891
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: Nishizawa Naoko 石川県立大学, 生物資源環境学部, 学長 (70156066)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: ニコチアナミン / ニコチアナミン分泌膜輸送体 / 重金属耐性植物 / 植物の金属栄養 / イネ / Arabidopsis halleri / 重金属耐性 / 植物 / トランスポーター / A.halleri

Outline of Final Research Achievements

Nicotianamine is a chelating substance that is present in all plants and plays an important role in the translocation of essential elements such as iron and zinc in plant body. In this study, we identified new efflux transporter of nicotianamine from a heavy metal-accumulating plant Arabidopsis helleri to secrete nicotianamine into the rhizosphere. This efflux transporter was shown to contribute to the tolerance for excess zinc in the soil. It is expected that it can be used as a method for cleaning up heavy metal-contaminated soil with plants.

Academic Significance and Societal Importance of the Research Achievements

イネ科植物はムギネ酸類を根から分泌し、土壌中の鉄を可溶化して吸収する。ムギネ酸類の生合成中間体であり、金属元素のキレーターであるニコチアナミンは植物体内で働き根圏には分泌されないとされてきた。重金属集積植物からニコチアナミン分泌膜輸送体を単離したことにより、ニコチアナミンも根圏に分泌され、過剰金属元素の無毒化に寄与している可能性が示された。根圏の過剰金属の無毒化に働くニコチアナミンの新たな機能が明らかとなり、植物栄養学分野に新しい展開をもたらした。またイネにおけるニコチアナミン分泌の機能とその果たす役割が明らかになり、必須栄養金属の代謝に新分野を切り開くこととなった。

Research Products

• [Journal Article] Physiological and transcriptomic analysis of responses to different levels of iron excess stress in various rice tissues, Soil Science and Plant Nutrition (2018)
  • Author(s): May Sann Aung, Hiroshi Masuda, Takanori Kobayashi & Naoko K. Nishizawa
  • Journal Title: Soil Science and Plant Nutrition Volume: in press Issue: 3 Pages: 370-385
  • DOI: 10.1080/00380768.2018.1443754
  • Peer Reviewed / Open Access
• [Journal Article] Rice HRZ ubiquitin ligases are crucial for response to excess iron (2018)
  • Author(s): Aung MS, Kobayashi T, Masuda H, Nishizawa NK
  • Journal Title: Physiol. Plant. Volume: in press Issue: 3 Pages: 282-296
  • DOI: 10.1111/ppl.12698
  • NAID: 120006942648
  • Peer Reviewed / Open Access
• [Journal Article] Iron-deficiency response and expression of genes related to iron homeostasis in poplars (2018)
  • Author(s): 3.Hiroshi Masuda, May Sann Aung, Keisuke Maeda, Takanori Kobayashi, Naoki Takata, Toru Taniguchi, Naoko Kishi Nishizawa
  • Journal Title: Journal of Soil Science and Plant Nutrition Volume: 64 Issue: 5 Pages: 576-588
  • DOI: 10.1080/00380768.2018.1480325
  • NAID: 210000012015
  • Peer Reviewed / Open Access
• [Journal Article] An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions. (2017)
  • Author(s): Yamauchi, T., Yoshioka, M., Fukazawa, A., Mori, H., Nishizawa, N.K., Tsutsumi, N., Yoshioka, H. and Nakazono, M.
  • Journal Title: The Plant Cell Volume: 印刷中 Issue: 4 Pages: 775-790
  • DOI: 10.1105/tpc.16.00976
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bioenergy grass [Erianthus ravennae (L.) Beauv.] secretes two members of mugineic acid family phytosiderophores which involved in their tolerance to Fe deficiency (2017)
  • Author(s): Nozoye Tomoko、Aung May Sann、Masuda Hiroshi、Nakanishi Hiromi、Nishizawa Naoko K.
  • Journal Title: Soil Science and Plant Nutrition Volume: 63 Issue: 6 Pages: 543-552
  • DOI: 10.1080/00380768.2017.1394168
  • Peer Reviewed / Open Access
• [Journal Article] The iron-chelate transporter OsYSL9 plays a role in iron (2017)
  • Author(s): Senoura T, Sakashita E, Kobayashi T, Takahashi M, Aung MS, Masuda H, Nakanishi H, Nishizawa NK.
  • Journal Title: Plant Mol Biol Volume: 79 Issue: 4-5 Pages: 1-13
  • DOI: 10.1007/s11103-017-0656-y
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of barley nicotianamine synthase 1 confers tolerance in the sweet potato to iron deficiency in calcareous soil. (2017)
  • Author(s): T. Nozoye, M. Otani, T. Senoura, H. Nakanishi, N.K. Nishizawa
  • Journal Title: Plant and Soil Volume: 印刷中 Issue: 1-2 Pages: 1-14
  • DOI: 10.1007/s11104-016-3134-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil. (2017)
  • Author(s): Hiroshi Masuda, Erika Shimoshi, Tatsuro Hamada, Takeshi Senoura, Takanori Kobayashi, May Sann Aung, Yasuhiro Ishimaru, Yuko Ogo, Naoko K. Nishizawa.
  • Journal Title: PLoS ONE Volume: 12(3) Issue: 3 Pages: e0173441-e0173441
  • DOI: 10.1371/journal.pone.0173441
  • Peer Reviewed / Open Access
• [Journal Article] Paralogs and mutants show that one DMA synthase functions in Fe homeostasis in rice. (2017)
  • Author(s): Khurram Bashir, Tomoko Nozoye, Seiji Nagasaka, Sultana Rasheed, Nanako Miyauchi, Motoaki Seki, Hiromi Nakanishi, Naoko K. Nishizawa.
  • Journal Title: Journal of Experimental Botany Volume: 68 Issue: 7 Pages: 1785-1795
  • DOI: 10.1093/jxb/erx065
  • Peer Reviewed / Open Access
• [Journal Article] Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots. (2016)
  • Author(s): Kobayashi, T. (corresponding author), Itai, R.N., Senoura, T., Oikawa, T., Ishimaru, Y., Ueda, M., Nakanishi, H., Nishizawa, N.K.
  • Journal Title: Plant Molecular Biology Volume: 印刷中 Issue: 4-5 Pages: 533-547
  • DOI: 10.1007/s11103-016-0486-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulating Subcellular Metal Homeostasis: The Key to Crop Improvement. (2016)
  • Author(s): Khurram Bashir, Sultana Rasheed, Takanori Kobayashi, Motoaki Seki, Naoko K. Nishizawa.
  • Journal Title: Frontiers in Plant Science Volume: 7 Pages: 1192-1192
  • DOI: 10.3389/fpls.2016.01192
  • Peer Reviewed / Open Access
• [Journal Article] Identification of mineral-rich rice varieties in Myanmar. (2016)
  • Author(s): May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Yusuke Kakei, Ye Tint Tun, Hiromi Nakanishi, Takashi Yamakawa, Naoko K. Nishizawa.
  • Journal Title: Myanmar Agricultural Research Journal Volume: 3 Pages: 14-29
  • Peer Reviewed / Open Access
• [Journal Article] The detection of endogenous 2’-deoxymugineic acid in olives (Olea europaea L.) indicates the biosynthesis of mugineic acid family phytosiderophores in non-graminaceous plants. (2016)
  • Author(s): M. Suzuki, T. Nozoye, S. Nagasaka, H. Nakanishi, N.K. Nishizawa, S. Mori
  • Journal Title: Soil Science and Plant Nutrition Volume: 62 Issue: 5-6 Pages: 481-488
  • DOI: 10.1080/00380768.2016.1230724
  • Peer Reviewed / Open Access
• [Presentation] Transporters crucial for metal transport and distribution in rice (2017)
  • Author(s): Naoko K. Nishizawa, Takeshi Senoura, Emi Sakashita, Takanori Kobayashi, Michiko Takahashi, Tomoko Nozoye, May Sann Aung, Hiroshi Masuda, Hiromi Nakanishi
  • Organizer: 2017 International Symposium on Rice Functional Genomics (ISRFG2017)
  • Int'l Joint Research / Invited
• [Presentation] HRZ ubiquitin ligases negatively regulate rice iron uptake, transport and accumulation under various iron conditions (2017)
  • Author(s): Takanori Kobayashi, May Sann Aung, Reiko Nakanishi Itai, Takeshi Senoura, Hiroshi Masuda, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa
  • Organizer: ASPB annual meeting in 2017 (Plant Biology 2017)
  • Int'l Joint Research
• [Presentation] An iron-chelate transporter OsYSL9 is involved in iron distribution in developing rice seeds (2017)
  • Author(s): Naoko K. Nishizawa, Takeshi Senoura, Takanori Kobayashi, Emi Sakashita, Hiromi Nakanishi
  • Organizer: ASPB annual meeting in 2017 (Plant Biology 2017)
  • Int'l Joint Research
• [Presentation] Rice HRZ ubiquitin ligases are involved in both iron deficiency and excess responses and jasmonate signaling (2017)
  • Author(s): Takanori Kobayashi, May Sann Aung, Reiko Nakanishi Itai, Takeshi Senoura, Hiroshi Masuda, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] The iron-chelate transporter OsYSL9 is crutial in iron distribution in developing rice grain (2017)
  • Author(s): Naoko K. Nishizawa, Takeshi Senoura, Emi Sakashita, Takanori Kobayashi, Michiko Takahashi, May Sann Aung, Hiroshi Masuda, Hiromi Nakanishi
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] Physiological and transcriptomic analysis of iron excess response in various tissues of rice (2017)
  • Author(s): May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Naoko K. Nishizawa
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] Iron biofortification in important rice varieties obtained by the introduction of multiple genes or ion-beam irradiation (2017)
  • Author(s): Hiroshi Masuda, May Sann Aung, Takanori Kobayashi, Hiromi Nakanishi, Naoko K. Nishizawa
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] Fe Acquisition by Mugineic Acid Family Phytosiderophores in Foxtail Millet (2017)
  • Author(s): Reiko Nakanishi Itai, Naoko K. Nishizawa, Hiromi Nakanishi
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] The metabolome analysis in Fe-deficient olive plants; possible biosynthesis of mugineic acid family phytosiderophores in nongraminaceous plants (2017)
  • Author(s): Motofumi Suzuki, Tomoko Nozoye, Seiji Nagasaka,Hiromi Nakanishi, Naoko K. Nishizawa, Satoshi Mori
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] Characterization of an Fe-deficiency Inducible Protein Kinase Gene (PK) in Rice (2017)
  • Author(s): Fan Wang, Reiko N. Itai, Takeshi Senoura, Naoko K. Nishizawa, Takashi Yamakawa, Hiromi Nakanishi
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] Barley nicotianamine synthase 1 (HvNAS1) gene is usuful candidate to improve nutritional qualities and agricultural productivity in soybean and sweet potato (2017)
  • Author(s): Tomoko Nozoye, Takeshi Senoura, Suyoen Kim, Yuske Kakei, Michiko Takahashi, Motoyasu Otani, Hiromi Nakanishi, Naoko K. Nishizawa
  • Organizer: XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
  • Int'l Joint Research
• [Presentation] イネのユビキチンリガーゼHRZは鉄過剰耐性に関与する (2017)
  • Author(s): メイ サン アウン・小林高範・増田寛志・西澤直子
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] Selection of iron toxicity-tolerant varieties among popular Myanmer rice (2017)
  • Author(s): May Sann Aung・増田寛志・小林高範・西澤直子
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] Characterizing the paralogs and mutants of OsDMAS1 (2017)
  • Author(s): Khurram Bashir・野副朋子・長坂征治・Sultana Rasheed・宮内菜奈子・関原明・中西啓仁・西澤直子
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] 鉄関連遺伝子 Gmferritin、HvNAS1、OsYSL2、IDS3 を同時導入した高鉄米の作出 (2017)
  • Author(s): メイサンアウン・増田寛志・小林高範・西澤直子
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] Characterization of an Fe-deficiency inducible protein kinase gene (PK) in rice (2017)
  • Author(s): Fan Wang・板井玲子・瀬野浦武志・西澤直子・山川隆・中西啓仁
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] The development of low-cadmium rice (2017)
  • Author(s): Naoko K. Nishizawa
  • Organizer: 台湾国立嘉義大学農学研究科セミナー
  • Invited
• [Presentation] Two metal chelators , phytosiderophore and nicotiananine are crucial to maintain metal homeostasis in crops (2017)
  • Author(s): Naoko K. Nishizawa
  • Organizer: 台湾中央研究院植物微生物学研究所セミナー
  • Invited
• [Presentation] The development of low-cadmium rice (2017)
  • Author(s): Naoko K. Nishizawa
  • Organizer: 台湾国立台湾大学生物資源農学研究科セミナー
  • Invited
• [Presentation] イネのユビキチンリガーゼ HRZ は鉄過剰条件でも重要である (2017)
  • Author(s): メイ サン アウン・小林高範・増田寛志・西澤直子
  • Organizer: 第58回日本植物生理学会年会
• [Presentation] Overexpression of barley nicotianamine synthase 1 (HvNAS1) confer tolerance to Fe deficiency on calcareous soil in soybean and sweet potato. (2016)
  • Author(s): Tomoko Nozoye, Takeshi Senoura, Suyeon Kim, Yusuke Kakei, Michiko Takahashi, Motoyasu Otani, Hiromi Nakanishi, Naoko K. Nishizawa.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] Efflux or uptake transporters involved in iron transport and distribution in rice plants. (2016)
  • Author(s): Takeshi Senoura, Tomoko Nozoye, Emi Sakashita, Hiromi Nakanishi, Takanori Kobayashi, Naoko K. Nishizawa.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] HRZ ubiquitin ligases and jasmonate signaling regulate iron deficiency responses in rice roots. (2016)
  • Author(s): Takanori Kobayashi, Reiko Nakanishi Itai, Takeshi Senoura, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] Detection of endogenous 2’-deoxymugineic acid in olive plant indicates biosynthesis of phytosiderophore in non-graminaeceous plants. (2016)
  • Author(s): Motofumi Suzuki, Tomoko Nozoye, Seiji Nagasaka, Hiromi Nakanishi, Naoko K. Nishizawa, Satoshi Mori.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] Producing high iron rice with important rice varieties. (2016)
  • Author(s): Hiroshi Masuda, May Sann Aung, Takanori Kobayashi, Hiromi Nakanishi, Naoko K. Nishizawa.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] Elucidation of the molecular mechanism of iron excess stress response in rice. (2016)
  • Author(s): May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Naoko K. Nishizawa.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] Varieties of mugineic acid family phytosiderophores secreted from Fe-deficient foxtail millet and gene expression related to their biosynthesis. (2016)
  • Author(s): Reiko Nakanishi Itai, Naoko K. Nishizawa, Hiromi Nakanishi.
  • Organizer: 18th International Symposium on Iron Nutrition and Interactions in Plants
  • Int'l Joint Research
• [Presentation] 作物のミネラル獲得戦略を明らかにして環境とヒトの健康に貢献する (2016)
  • Author(s): 西澤直子
  • Organizer: 平成28年度女子栄養大学香友会公開講座
  • Invited
• [Presentation] 鉄トランスポーター OsYSL9 の機能解析 (2016)
  • Author(s): 瀬野浦武志・坂下絵美・小林高範・May Sann Aung・増田寛志・中西啓仁・西澤直子
  • Organizer: 日本土壌肥料学会2016年度佐賀大会
• [Presentation] 三価鉄還元能とムギネ酸類合成能のさらなる強化による鉄欠乏耐性イネの作出 (2016)
  • Author(s): 結城麟太郎・瀬野浦武志・増田寛志・野副朋子・板井玲子・中西啓仁・小林高範・西澤直子
  • Organizer: 日本土壌肥料学会2016年度佐賀大会
• [Presentation] 重イオンビーム照射による鉄・亜鉛栄養価の高いコシヒカリの作出 (2016)
  • Author(s): 増田寛志・May Sann Aung・小林高範・中西啓仁・西澤直子
  • Organizer: 日本土壌肥料学会2016年度佐賀大会
• [Presentation] Molecular mechanism of iron excess response in various parts of rice. (2016)
  • Author(s): May Sann Aung、増田寛志、小林高範、西澤直子
  • Organizer: 日本土壌肥料学会2016年度佐賀大会
• [Presentation] 植物における鉄の役割－植物の鉄吸収メカニズムとその利用 (2016)
  • Author(s): 西澤直子
  • Organizer: 2016年WINEP講演会
  • Invited
• [Remarks] 石川県立大学 生物資源工学研究所 植物細胞工学研究室
  • URL: http://ribb.ishikawa-pu.ac.jp/pct/index.htm
• [Remarks] 石川県立大学 生物資源工学研究所 植物細胞工学研究室ホームページ
  • URL: http://ribb.ishikawa-pu.ac.jp/pct/index.htm