| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
|
|---|
| Outline of Final Research Achievements |
Genome editing is a new breeding technology that imparts useful traits by deleting bases of targeted genes. In this study, we developed a genome editing technology of Cryptomeria japonica using the CRISPR/Cas9 system and evaluated its efficiency. In addition, we selected target genes for pollen-free C. japonica, and prepared C. japonica whose target genes were edited. Furthermore, in order to make elite tree of C. japonica pollen-free, we constructed a bioresource in which cells capable of plant regeneration by somatic embryogenesis were stored in liquid nitrogen.
|
