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Development of diagnostic tools for growth and stress for salmon aquaculture

Research Project

Project/Area Number 16H04966
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionHokkaido University

Principal Investigator

Shimizu Munetaka  北海道大学, 水産科学研究院, 准教授 (90431337)

Co-Investigator(Kenkyū-buntansha) 棟方 有宗  宮城教育大学, 教育学部, 准教授 (10361213)
森山 俊介  北里大学, 海洋生命科学部, 教授 (50222352)
内田 勝久  宮崎大学, 農学部, 教授 (50360508)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords成長 / ホルモン / サケ科魚類 / 海面養殖 / 指標 / 海水適応能 / 水産学 / ストレス
Outline of Final Research Achievements

This project aimed to understand the mechanism of growth by insulin-like growth factor (IGF)-I and its binding proteins (IGFBPs) and utilize them as growth indices in aquaculture. We produced recombinant salmon IGFBP-1a and -1b, two major circulating forms, and showed they had an inhibitory action on IGF-I. We next established an immunoassay for IGFBP-1a using recombinant proteins as antigen and assay components, and revealed that serum IGFBP-1a is negatively correlated with individual growth rate. Our result suggests that IGFBP-1a is useful as an inverse index of growth in salmon.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義は、インスリン様成長因子結合蛋白(IGFBP)の組換え蛋白を作製する方法とその機能を解析する実験系を確立したてんである。これにより、これまで行われていなかったIGFBPによる魚類の成長調節メカニズムの解析を可能となった。社会的意義として、血中IGFBPの測定系を確立し、2種類のIGFBPが、増養殖業の現場において飼育魚の成長停滞もしくはストレスの指標として有用であることを示した点が挙げられる。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (50 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (7 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (22 results) (of which Int'l Joint Research: 9 results,  Invited: 8 results) Book (12 results) Remarks (1 results)

  • [Int'l Joint Research] ノース研究所(ノルウェー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 冷水性養殖研究所(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Uni Research(ノルウェー)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] NOAA Fisheries/Agricultural Research Service, USDA(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Uni Research(ノルウェー)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] NOAA Fisheries(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Fisheries and Oceans Canada(カナダ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Editing the duplicated insulin-like growth factor binding protein-2b gene in rainbow trout (Oncorhynchus mykiss)2018

    • Author(s)
      Cleveland, B.M., Yamaguchi, G., Radler, L.M., and Shimizu, M.
    • Journal Title

      Scientific Report

      Volume: 8 Issue: 1 Pages: 16054-16054

    • DOI

      10.1038/s41598-018-34326-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Assessment of the timing and degree of smolt development in southern populations of masu salmon2018

    • Author(s)
      Inatani, Y., Ineno, T., Sone, S., Matsumoto, N., Uchida, K., and Shimizu, M.
    • Journal Title

      Journal of Fish Biology

      Volume: 93 Issue: 3 Pages: 490-500

    • DOI

      10.1111/jfb.13647

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison between Atlantic salmon Salmo salar post-smolts reared in open sea cages and in the Preline raceway semi-closed containment aquaculture system2018

    • Author(s)
      Balseiro, P., Moe, O., Gamlem, I., Shimizu, M., Sveier, H., Nilsen, T.O., Kaneko, N., Ebbesson, L., Pedrosa, C., Tronci, V., Nylund, A., and Handeland, S.O.
    • Journal Title

      Journal of Fish Biology

      Volume: 93 Issue: 3 Pages: 567-579

    • DOI

      10.1111/jfb.13659

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Changes in glycogen concentration and gene expression levels of glycogen-metabolizing enzymes in muscle and liver of developing masu salmon2018

    • Author(s)
      Fumiya Furukawa, Shotaro Irachi, Mugen Koyama, Otto Baba, Hajime Akimoto, Sei-ichi Okumura, Hirohiko Kagawa, Katsuhisa Uchida
    • Journal Title

      Comparative Biochemistry and Physiology, Part A

      Volume: 225 Pages: 74-82

    • DOI

      10.1016/j.cbpa.2018.07.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stress up-regulates oxidative burst in juvenile Chinook salmon leukocytes2018

    • Author(s)
      Herron, C., Cogliati, K.M., Dolan, B.P., Munakata, A., Schreck, C.B.
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 80 Pages: 655-659

    • DOI

      10.1016/j.fsi.2018.06.038

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Production of recombinant salmon insulin-like growth factor binding protein-1 subtypes2018

    • Author(s)
      Tanaka Hanae、Oishi Gakuto、Nakano Yusuke、Mizuta Hiroko、Nagano Yuta、Hiramatsu Naoshi、Ando Hironori、Shimizu Munetaka
    • Journal Title

      General and Comparative Endocrinology

      Volume: 257 Pages: 184-191

    • DOI

      10.1016/j.ygcen.2017.06.015

    • NAID

      120006551587

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of seawater reared on the nutritional composition and antioxidant activity of edible muscle in smoltified-landlocked masu salmon (Oncorhynchus masou masou)2018

    • Author(s)
      Tanaka Ryusuke, Uchida Katsuhisa, Ishimaru Mami, Ito Meiko, Matsumoto Naoto, Taoka Yousuke, Hatate Hideo
    • Journal Title

      Journal of Food Measurement and Characterization

      Volume: 12 Issue: 1 Pages: 200-208

    • DOI

      10.1007/s11694-017-9631-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circulating insulin-like growth factor binding proteins in fish: their identities and physiological regulation2017

    • Author(s)
      Shimizu, M., and Dickhoff, W.W.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 252 Pages: 150-161

    • DOI

      10.1016/j.ygcen.2017.08.002

    • NAID

      120006523744

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Insulin-like growth factor binding protein-2b in salmon: its identity, utility as growth index and possible role in growth2019

    • Author(s)
      Shimizu, M., Kizaki, R., Beckman, B.R., and Dickhoff, W.W.
    • Organizer
      Aquaculture 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サケマスの成長指標としてのインスリン様成長因子結合蛋白2018

    • Author(s)
      清水宗敬
    • Organizer
      サケマスの生理・生態学研究の最前線
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] サクラマスの組換えインスリン様成長因子結合蛋白-1の作製2018

    • Author(s)
      長谷川竜也・田中英絵・木﨑亮佑・佐藤萌絵・清水宗敬
    • Organizer
      第12回サケ学研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] サケ科魚類の成長や海水適応能を促す飼料の開発2018

    • Author(s)
      森山俊介
    • Organizer
      サケマスの生理・生態学研究の最前線
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 太平洋サケの銀化変態期可変性の機構を探る2018

    • Author(s)
      棟方有宗
    • Organizer
      サケマスの生理・生態学研究の最前線
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 太平洋サケのストレス応答と回遊行動の分化2018

    • Author(s)
      棟方有宗
    • Organizer
      第43回日本比較内分泌学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Mechanisms and roles of body color changes during smoltification in Pacific salmon2018

    • Author(s)
      Munakata, A.
    • Organizer
      8th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 日本最南限におけるサクラマスの養殖の現状と課題、将来展望2018

    • Author(s)
      内田勝久
    • Organizer
      サケマスの生理・生態学研究の最前線
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] みやざきサクラマスの生産を通じた地域との連携2018

    • Author(s)
      内田勝久
    • Organizer
      国内サーモン養殖による地域振興に向けた課題と展望
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of oral administration of salmon growth hormone on somatic growth of masu salmon (Oncorhynchus masou) fry2018

    • Author(s)
      Ueno, K., Moriyama, S., Miyanishi, H., Kagawa, H., and Uchida, K.
    • Organizer
      8th Annual Seminar between University of Miyazaki and Pukyong National University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海面養殖に適した宮崎県産ヤマメ種苗の選抜育種の試み2018

    • Author(s)
      上野賢・上林大介・宮西弘・香川浩彦・内田勝久
    • Organizer
      平成30年度日本水産学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Endocrine aspects of landlocked masu salmon in freshwater and seawater aquaculture conditions: their smoltification, seawater adaptation, growth and reproduction2018

    • Author(s)
      Ueno, K., Miyanishi, H., Kagawa, H., and Uchida, K.
    • Organizer
      8th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サケ幼稚魚のリアルタイム成長評価のための技術開発2018

    • Author(s)
      清水宗敬
    • Organizer
      平成30年度日本水産学会春季大会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 稚魚の生理状態の評価2018

    • Author(s)
      森山俊介・清水恵子
    • Organizer
      平成30年日本水産学会春季大会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Effects of salmon Gh-added diet on growth and SW adaptability of FW-reared masu salmon (Oncorhynchus masou)2018

    • Author(s)
      Uchida, K., and Ueno, K.
    • Organizer
      2nd Joint Symposium on Aquaculture Science between University of Miyazaki, Japan and National Institute of Fisheries Science, Korea 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サクラマスインスリン様成長因子結合蛋白-1の機能的組換え蛋白の作製2017

    • Author(s)
      田中英絵・中野裕介・水田紘子・平松尚志・清水宗敬
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 血中インスリン様成長因子結合蛋白-1bを用いたシロザケ稚魚の成長評価2017

    • Author(s)
      金子信人・虎尾 充・藤原 真・越野陽介・宮腰靖之・清水宗敬
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Unraveling the roles of circulating insulin-like growth factor binding proteins in fish growth2017

    • Author(s)
      Shimizu, M.
    • Organizer
      International Symposium on Fisheries Sciences for Future Generations
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Assessment of the timing of "smoltification" in masu salmon in Miyazaki, south Japan2017

    • Author(s)
      Inatani, Y., Ineno, T., Sone, S., Matsumoto, N., Uchida, K., and Shimizu, M.
    • Organizer
      10th International Symposium on Salmon Smoltification
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サケ稚魚の初期生活史におけるインスリン様成長因子の機能2017

    • Author(s)
      森山俊介
    • Organizer
      平成29年度岩手県サケマス増殖協会点さけ増殖技術検討会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 陸封型ヤマメの銀化変態に伴う下垂体-甲状腺機能の発現動態2017

    • Author(s)
      伊良知正太郎・竹田 萌・宮西 弘・香川浩彦・内田勝久
    • Organizer
      日本動物学会第88回富山大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Production of recombinant salmon insulin-like growth factor binding protein-1 subtypes2016

    • Author(s)
      Munetaka Shimizu, Hanae Tanaka, Gakuto Oishi, Yusuke Nakano, and Hiroko Mizuta
    • Organizer
      8th International Symposium on Fish Endocrinology
    • Place of Presentation
      Lindholmen Conference Center, Gothenburg, Sweden
    • Year and Date
      2016-06-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] サクラマス その生涯と生活史戦略(10)サクラマスの利活用(章分担)2019

    • Author(s)
      棟方有宗
    • Total Pages
      5
    • Publisher
      生物研究社
    • Related Report
      2018 Annual Research Report
  • [Book] サクラマス その生涯と生活史戦略(9)サクラマスの資源減少(章分担)2018

    • Author(s)
      棟方有宗
    • Total Pages
      4
    • Publisher
      生物研究社
    • Related Report
      2018 Annual Research Report
  • [Book] サクラマス その生涯と生活史戦略(8)サクラマス群の成魚の河川内における生態(章分担)2018

    • Author(s)
      棟方有宗
    • Total Pages
      4
    • Publisher
      生物研究社
    • Related Report
      2018 Annual Research Report
  • [Book] サクラマス その生涯と生活史戦略(7)サクラマス群の稚魚の河川内における生態(章分担)2018

    • Author(s)
      棟方有宗
    • Total Pages
      5
    • Publisher
      生物研究社
    • Related Report
      2018 Annual Research Report
  • [Book] サクラマス その生涯と生活史戦略(6)遡上回遊・産卵行動の生理的調節機構(章分担)2018

    • Author(s)
      棟方有宗
    • Total Pages
      4
    • Publisher
      生物研究社
    • Related Report
      2018 Annual Research Report
  • [Book] サクラマス その生涯と生活史戦略(5)降河回遊行動の生理的調節機構(章分担)2018

    • Author(s)
      棟方有宗
    • Total Pages
      4
    • Publisher
      生物研究社
    • Related Report
      2018 Annual Research Report
  • [Book] アグリバイオ 2 (4)(章分担:サクラマスの回遊多型と種苗生産)2018

    • Author(s)
      棟方有宗
    • Publisher
      北隆館
    • Related Report
      2017 Annual Research Report
  • [Book] 海洋と生物 40 (1)(章分担:サクラマス その生涯と生活史戦略(4))2018

    • Author(s)
      棟方有宗
    • Publisher
      生物研究社
    • Related Report
      2017 Annual Research Report
  • [Book] 月刊アクアネット 237号(章分担:日本最南限でのサクラマス海面養殖の取り組み・五ヶ瀬産の銀化ヤマメを海で育む)2018

    • Author(s)
      内田勝久
    • Publisher
      湊分社
    • Related Report
      2017 Annual Research Report
  • [Book] 海洋と生物 39 (6)(章分担:サクラマス その生涯と生活史戦略(3))2017

    • Author(s)
      棟方有宗
    • Publisher
      生物研究社
    • Related Report
      2017 Annual Research Report
  • [Book] 海洋と生物 39 (5)(章分担:サクラマス その生涯と生活史戦略(2))2017

    • Author(s)
      棟方有宗
    • Publisher
      生物研究社
    • Related Report
      2017 Annual Research Report
  • [Book] 海洋と生物 39 (4)(章分担:サクラマス その生涯と生活史戦略(1))2017

    • Author(s)
      棟方有宗
    • Publisher
      生物研究社
    • Related Report
      2017 Annual Research Report
  • [Remarks] Munetaka Shimizu, Ph.D.

    • URL

      http://www.geocities.jp/fishvitellogenin/ms/Welcome.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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