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Mechanisms associated with resistance of Adoxophyes honmai against baculoviruses

Research Project

Project/Area Number 16H05052
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Nakai Madoka  東京農工大学, (連合)農学研究科(研究院), 教授 (60302907)

Co-Investigator(Kenkyū-buntansha) 佐藤 令一  東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
高務 淳  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (80399378)
石井 一夫  東京農工大学, (連合)農学研究科(研究院), 特任教授 (60449238)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsバキュロウイルス / 抵抗性 / 選抜 / 核多角体病ウイルス / 顆粒病ウイルス / 中腸上皮細胞 / 交差抵抗性 / チャノコカクモンハマキ / 機構解明 / 生物的防除 / チョウ目 / メカニズム
Outline of Final Research Achievements

To elucidate resistance mechanism of insect against baculoviruses, the resistant strain (R-strain)of Adoxophyes honmai (Tortricidae) were selected by continuous exposure to a nucleopolyhedrovirus (Adoxophyes honmai NPV: AdhoNPV). Comparative studies for R-strain and non-selected susceptible strain (S-strain) revealed that plural resistant mechanisms were exists including decreased binding of occlusion derived viruses to midgut epithelial cells.

Academic Significance and Societal Importance of the Research Achievements

害虫の薬剤抵抗性発達は、害虫防除の研究における最も重要な課題のひとつであるため、本研究の成果は、社会的意義がある。害虫の微生物防除資材は、これまで害虫に抵抗性が発達しにくい資材であると考えられてきた。しかし、2005年に欧州でバキュロウイルスに対して抵抗性をもつ野外害虫個体群が世界ではじめて出現した。抵抗性メカニズムの解明は、バキュロウイルス の抵抗性マネージメントに利用できる。また、バキュロウイルスの感染は、宿主昆虫の中腸におけるウイルス粒子の結合により開始される。本研究により確立した抵抗性昆虫個体群は、バキュロウイルス感染機構を解明するモデル昆虫としての学術的価値がある。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (29 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (8 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] Simon Fraser University(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Julius Kuehn Institute(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Simon Fraser University(カナダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Julius Kuehn Institute(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Julius Kuhn Institute(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Alameda County Mosquito Abatement Dist(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Julius Kuehn Institute(Germany)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Simon Fraser University(Canada)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Complete Genome Sequence of an Alphabaculovirus from Choristoneura diversana2020

    • Author(s)
      Jun Takatsuka
    • Journal Title

      Microbiology Resource Announcement

      Volume: 9 Issue: 10

    • DOI

      10.1128/mra.00051-20

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphological properties of the occlusion body of Adoxophyes orana granulovirus2018

    • Author(s)
      Keiko Tsuruta, Joerg T. Wennmann, Yasuhisa Kunimi, Maki N. Inoue, Madoka Nakai
    • Journal Title

      Journal of Invertebrate Pathology

      Volume: 154 Pages: 58-64

    • DOI

      10.1016/j.jip.2018.03.016

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Acquired resistance to a nucleopolyhedrovirus in the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae) after selection by serial viral administration.2017

    • Author(s)
      Nakai, M., Takahashi, K., Iwata, K., Tanaka, K., Koyanagi, J., Ookuma, A., Takatsuka, J, Okuno, S., Kunimi, Y.
    • Journal Title

      Journal of Invertebrate Pathology,

      Volume: 145, Pages: 23-30

    • DOI

      10.1016/j.jip.2017.03.003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Midgut-based resistance to oral infection by a nucleopolyhedrovirus in the laboratory-selected strain of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae).2017

    • Author(s)
      Iwata, K., Haas-Stapleton,, E., Inoue, M.N., Kunimi. Y., Nakai, M. (2017)
    • Journal Title

      Journal of General Virology

      Volume: 98, Issue: 2 Pages: 296-304

    • DOI

      10.1099/jgv.0.000684

    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Step-by-step acquired resistance of Adoxophyes honmai passaged by nucleopolyhedrovirus: resistant mechanism and inheritance trait2019

    • Author(s)
      Moriyasu Tatsuzawa, Jun Takatsuka, Maki N. Inoue, Yasuhisa Kunimi, Madoka Nakai
    • Organizer
      Society for invertebrate pathology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] バキュロウイルス抵抗性チャノコカクモンハマキ系統の抵抗性発達過程の調査2018

    • Author(s)
      立澤杜泰・井上真紀・仲井まどか
    • Organizer
      第13回昆虫病理研究会シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 核多角体病ウイルスにより選抜したチャノコカクモンハマキ個体群の 顆粒病ウイルスへの交差抵抗性とその遺伝様式2018

    • Author(s)
      嶋倉直也 ・ 井上真紀・ 仲井まどか
    • Organizer
      第13回昆虫病理研究会シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 核多角体病ウイルスにより選抜したチャノコカクモンハマキの段階的抵抗性発達と交雑実験による抵抗性遺伝様式の推定2018

    • Author(s)
      仲井まどか・立澤杜泰・四宮啓登・高橋和宏・高務淳・井上真紀・国見裕久
    • Organizer
      第62回日本応用動物昆虫学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] チャノコカクモンハマキ核多角体ウイルス抵抗性系統の抵抗性機構の発達様式調査2018

    • Author(s)
      立澤 杜泰・井上 真紀・仲井 まどか
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Madoka Nakai2018

    • Author(s)
      Ecology of insect viruses: how viruses adapt to their insect hostpopulation
    • Organizer
      50th of Annual Meeting and Golden Jubilee Celebration of the Society for Invertebrate Pathology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Deciphering the genetic factor for morphologygranulovirus occlusionbody2018

    • Author(s)
      Keiko Tsuruta, Joerg T. Wennmann, Maki N. Inoue, YasuhisaKunimi, Johannes A. Jehle, Madoka Nakai
    • Organizer
      50th of Annual Meeting and Golden Jubilee Celebration of the Society for Invertebrate Pathology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of resistance to baculoviruses in a lepidopteran insect2017

    • Author(s)
      Madoka Nakai
    • Organizer
      2017 Spring Conference of the Korean Society for Applied Entomology
    • Place of Presentation
      Gyeongju Korea
    • Year and Date
      2017-04-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 核多角体病ウイルス選抜チャノコカクモンハマキ系統の顆粒病ウイルスに対する交差抵抗性の抵抗性機構と遺伝様式2017

    • Author(s)
      岩田 賢人・鶴田 景子・国見 裕久・井上 真紀・仲井 まどか
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] チャノコカクモンハマキ核多角体ウイルス抵抗性チャノコカクモンハマキ系統のAutographa californica核多角体病ウイルスに対する交差 抵抗性2017

    • Author(s)
      立澤 杜泰 ・岩田 賢人・高務 淳 ・井上 真紀 ・國見 裕久 ・仲井 まどか
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 昆虫のバキュロウイルスに対する 抵抗性メカニズムの解明2016

    • Author(s)
      岩田賢人, 石井一夫, 古崎利紀, 水谷哲也,大松勉, 長井誠, 齋藤康将, 井上真紀, 仲井まどか
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場-ANA クラウンプラザ富山(富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 核多角体病ウイルス抵抗性チャノコカクモンハマキ系統におけるRNA-Seqを用 いた遺伝子発現解析2016

    • Author(s)
      岩田賢人, 石井一夫, 古崎利紀, 水谷哲也, 大松勉, 長井誠,井上真紀, 国見裕久, 仲井まどか
    • Organizer
      第12回 昆虫病理研究会シンポジウム
    • Place of Presentation
      モンタナリゾート(宮城県岩沼市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 核多角体病ウイルスで選抜した抵抗性チャノコカクモンハマキ系統は顆粒病ウイルスに対して異なる抵抗性メカニズムを持つ2016

    • Author(s)
      岩田賢人, Eric Haas-Stapleton, 井上真紀, 国見裕久, 仲井まどか
    • Organizer
      第12回 昆虫病理研究会シンポジウム
    • Place of Presentation
      モンタナリゾート(宮城県岩沼市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of a novel mode of resistance against Cydia pomonella granulovirus in codling moth indicates a highly dynamic adaptation in the host population,2016

    • Author(s)
      Annette Sauer, Petr Nguyen, Eva Fritsch, Karin Undorf-Spahn1, Kento Iwata, Madoka Nakai, David Heckel, Frantisek Marec, Johannes Jehle
    • Organizer
      49th of Annual meeting of the Society for Invertebrate Pathology,
    • Place of Presentation
      Tours, France
    • Year and Date
      2016-07-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Resistance to baculoviruses in the midgut of Adoxophyes honmai,2016

    • Author(s)
      Kento Iwata, Yasuhisa Kunimi, Maki N. Inoue, Madoka Nakai (2016)
    • Organizer
      49th of Annual meeting of the Society for Invertebrate Pathology,
    • Place of Presentation
      Tours, France
    • Year and Date
      2016-07-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] Natural Enemies second edition (Box11.1)2018

    • Author(s)
      Ann E. Hajek, Jorgen Eilenberg
    • Total Pages
      439
    • Publisher
      Cambridge University Press
    • ISBN
      9781107668249
    • Related Report
      2018 Annual Research Report
  • [Remarks] 東京農工大学応用遺伝生態学研究室

    • URL

      http://www.tuat.ac.jp/~insect

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-01-28  

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