Mechanisms associated with resistance of Adoxophyes honmai against baculoviruses
| Project/Area Number |
16H05052
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Nakai Madoka 東京農工大学, (連合)農学研究科(研究院), 教授 (60302907)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 令一 東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
高務 淳 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (80399378)
石井 一夫 東京農工大学, (連合)農学研究科(研究院), 特任教授 (60449238)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | バキュロウイルス / 抵抗性 / 選抜 / 核多角体病ウイルス / 顆粒病ウイルス / 中腸上皮細胞 / 交差抵抗性 / チャノコカクモンハマキ / 機構解明 / 生物的防除 / チョウ目 / メカニズム |
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| Outline of Final Research Achievements |
To elucidate resistance mechanism of insect against baculoviruses, the resistant strain （R-strain）of Adoxophyes honmai (Tortricidae) were selected by continuous exposure to a nucleopolyhedrovirus (Adoxophyes honmai NPV: AdhoNPV). Comparative studies for R-strain and non-selected susceptible strain (S-strain) revealed that plural resistant mechanisms were exists including decreased binding of occlusion derived viruses to midgut epithelial cells.
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| Academic Significance and Societal Importance of the Research Achievements |
害虫の薬剤抵抗性発達は、害虫防除の研究における最も重要な課題のひとつであるため、本研究の成果は、社会的意義がある。害虫の微生物防除資材は、これまで害虫に抵抗性が発達しにくい資材であると考えられてきた。しかし、2005年に欧州でバキュロウイルスに対して抵抗性をもつ野外害虫個体群が世界ではじめて出現した。抵抗性メカニズムの解明は、バキュロウイルス の抵抗性マネージメントに利用できる。また、バキュロウイルスの感染は、宿主昆虫の中腸におけるウイルス粒子の結合により開始される。本研究により確立した抵抗性昆虫個体群は、バキュロウイルス感染機構を解明するモデル昆虫としての学術的価値がある。
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Acquired resistance to a nucleopolyhedrovirus in the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae) after selection by serial viral administration.2017
Author(s)
Nakai, M., Takahashi, K., Iwata, K., Tanaka, K., Koyanagi, J., Ookuma, A., Takatsuka, J, Okuno, S., Kunimi, Y.
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Journal Title
Journal of Invertebrate Pathology,
Volume: 145,
Pages: 23-30
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Midgut-based resistance to oral infection by a nucleopolyhedrovirus in the laboratory-selected strain of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae).2017
Author(s)
Iwata, K., Haas-Stapleton,, E., Inoue, M.N., Kunimi. Y., Nakai, M. (2017)
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Journal Title
Journal of General Virology
Volume: 98,
Issue: 2
Pages: 296-304
DOI
Related Report
Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Madoka Nakai2018
Author(s)
Ecology of insect viruses: how viruses adapt to their insect hostpopulation
Organizer
50th of Annual Meeting and Golden Jubilee Celebration of the Society for Invertebrate Pathology
Related Report
Int'l Joint Research / Invited
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[Presentation] Identification of a novel mode of resistance against Cydia pomonella granulovirus in codling moth indicates a highly dynamic adaptation in the host population,2016
Author(s)
Annette Sauer, Petr Nguyen, Eva Fritsch, Karin Undorf-Spahn1, Kento Iwata, Madoka Nakai, David Heckel, Frantisek Marec, Johannes Jehle
Organizer
49th of Annual meeting of the Society for Invertebrate Pathology,
Place of Presentation
Tours, France
Year and Date
2016-07-27
Related Report
Int'l Joint Research
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