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Development of a high accuracy detection method for cancer micro-metastasis in lymph nodes

Research Project

Project/Area Number 16H05168
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionTohoku University

Principal Investigator

Gonda Kohsuke  東北大学, 医学系研究科, 教授 (80375435)

Co-Investigator(Kenkyū-buntansha) 亀井 尚  東北大学, 医学系研究科, 教授 (10436115)
梅田 みか (渡辺みか)  東北大学, 大学病院, 准教授 (20292344)
大内 憲明  東北大学, 医学系研究科, 客員教授 (90203710)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywordsがん / リンパ節 / 転移 / 蛍光 / X線 / ナノ粒子 / イメージング / X線 / 癌 / 診断
Outline of Final Research Achievements

In cancer, the sentinel lymph node (SLN) is defined as a first node which receives lymph flow from cancer site. Therefore SLNs become the first location of cancer cells in lymph node metastasis. To achieve early diagnosis of cancer metastasis, it is important to detect location of true SLNs and evaluate accurate histological location of cancer cells in SLN with high accuracy. To develop new SLN diagnostic method, we here prepared multimodal nanoparticles with X-ray absorption and fluorescence. These nanoparticles enabled us to simultaneously-visualize an identical SLN in tumor-bearing mice with X-ray Computed Tomography and fluorescent imaging. Moreover, by estimating nanoparticles distribution in SLN utilizing the measurement of nanoparticles number with both imaging, we succeeded in precisely identifying the inflow of afferent lymph vessels into the sentinel lymph node, where lymph node metastasis begins. These results suggest that this method became better SLN diagnosis.

Academic Significance and Societal Importance of the Research Achievements

がん患部からのリンパ液が最初に流れ着くリンパ節(センチネルリンパ節)に注目し、リンパ節内のがん細胞の有無を高確度で調べることは、がん転移早期診断や根治にとって極めて重要である。本研究では、がんが転移した真のセンチネルリンパ節を蛍光とX線を使って正確に同定するための基盤技術の開発を行った。またリンパ節内に微小転移したがんを逃さず検出する方法の基盤技術開発にも成功した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (19 results)

All 2019 2018 2017 2016 Other

All Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 7 results) Remarks (1 results)

  • [Journal Article] Automated Quantification of Extranuclear ERα using Phosphor-integrated Dots for Predicting Endocrine Therapy Resistance in HR+/HER2? Breast Cancer2019

    • Author(s)
      Guo、Tada、Kitamura、Hamada、Miyashita、Harada-Shoji、Sato、Hamanaka、Tsuboi、Harada、Takano-Kasuya、Okada、Nakano、Ohuchi、Hayashi、Ishida、Gonda
    • Journal Title

      Cancers

      Volume: 11 Issue: 4 Pages: 526-526

    • DOI

      10.3390/cancers11040526

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy2019

    • Author(s)
      Hatoyama Keiichiro、Kitamura Narufumi、Takano-Kasuya Mayumi、Tokunaga Masayuki、Oikawa Takahiro、Ohta Mineto、Hamada Yoh、Tada Hiroshi、Kobayashi Yoshio、Kamei Takashi、Gonda Kohsuke
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 508 Issue: 4 Pages: 1093-1100

    • DOI

      10.1016/j.bbrc.2018.12.016

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in‐vivo fluorescence imaging2018

    • Author(s)
      Shindo Yuta、Inose Tomoya、Kubota Yohsuke、Oikawa Takahiro、Tokunaga Masayuki、Kamei Takashi、Gonda Kohsuke、Kobayashi Yoshio
    • Journal Title

      Materials Chemistry and Physics

      Volume: 220 Pages: 201-207

    • DOI

      10.1016/j.matchemphys.2018.08.073

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fabrication and dual imaging properties of quantum dot/silica core-shell particles immobilized with gold nanoparticles2018

    • Author(s)
      Li Ting-ting、Inose Tomoya、Oikawa Takahiro、Tokunaga Masayuki、Hatoyama Keiichiro、Nakashima Kouichi、Kamei Takashi、Gonda Kohsuke、Kobayashi Yoshio
    • Journal Title

      Materials Technology

      Volume: 33 Issue: 11 Pages: 737-747

    • DOI

      10.1080/10667857.2018.1502504

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermo-triggered Release of CRISPR-Cas9 System by Lipid-Encapsulated Gold Nanoparticles for Tumor Therapy2018

    • Author(s)
      Wang Peng、Zhang Lingmin、Zheng Wenfu、Cong Liman、Guo Zhaorong、Xie Yangzhouyun、Wang Le、Tang Rongbing、Feng Qiang、Hamada Yoh、Gonda Kohsuke、Hu Zhijian、Wu Xiaochun、Jiang Xingyu
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 57 Issue: 6 Pages: 1491-1496

    • DOI

      10.1002/anie.201708689

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 蛍光ナノ粒子を用いたがん組織の高感度定量イメージング2018

    • Author(s)
      権田幸祐、宮下穣、多田寛、中野寧
    • Journal Title

      がん分子標的治療

      Volume: 16 Pages: 59-64

    • Related Report
      2018 Annual Research Report
  • [Journal Article] がん病態の定量的ナノバイオイメージング2018

    • Author(s)
      権田幸祐、古澤直子、中野寧、小林芳男
    • Journal Title

      DDS学会Drug Delivery System誌

      Volume: 33 Pages: 87-97

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 1粒子蛍光イメージングによる高精度がん組織診断法2018

    • Author(s)
      権田幸祐、中野寧
    • Journal Title

      ファルマシア

      Volume: 54 Pages: 36-40

    • NAID

      130006301412

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Quantitative diagnostic imaging of cancer tissues by using phosphor-integrated dots with ultra-high brightness.2017

    • Author(s)
      Gonda Kohsuke
    • Journal Title

      scientific reports

      Volume: - Issue: 1 Pages: 7509-7509

    • DOI

      10.1038/s41598-017-06534-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative diagnosis of HER2 protein expressing breast cancer by single particle quantum dot imaging.2016

    • Author(s)
      Miyashita M, Gonda K, Tada H, Watanabe M, Kamei T, Sasano H, Ishida T, Ohuchi N.
    • Journal Title

      Cancer Medicine

      Volume: 5 Issue: 10 Pages: 2813-2824

    • DOI

      10.1002/cam4.898

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] X-ray computed tomography imaging of a tumor with high sensitivity using gold nanoparticles conjugated to a cancer-specific antibody via polyethylene glycol chains on their surface2016

    • Author(s)
      Tomohiko Nakagawa, Kohsuke Gonda, Takashi Kamei, Liman Cong, Yoh Hamada, Narufumi Kitamura, Hiroshi Tada, Takanori Ishida, Takuji Aimiya, Naoko Furusawa, Yasushi Nakano, Noriaki Ohuchi
    • Journal Title

      Science and Technology of Advanced Materials

      Volume: 17 Issue: 1 Pages: 387-397

    • DOI

      10.1080/14686996.2016.1194167

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 機能性ナノ粒子で迫るがん病態の可視化2018

    • Author(s)
      権田幸祐
    • Organizer
      第8回ナノカーボンバイオシンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 機能性ナノ粒子を用いたがん抗体医薬の薬効イメージング2018

    • Author(s)
      権田幸祐、徳永正之、古澤直子、中野寧
    • Organizer
      第27回日本バイオイメージング学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] がん病態のナノバイオイメージングとDDSへの展開2018

    • Author(s)
      権田幸祐、太田嶺人、根岸洋、徳永正之、古澤直子、中野寧
    • Organizer
      第34回DDS学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Quantitative diagnostic imaging of cancer tissues by using fluorescence nanoparticles with ultra-high brightness.2017

    • Author(s)
      Gonda K.
    • Organizer
      Tohoku University-KIST Joint Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nano-biomaging of cancer and peripheral artery disease using X-ray CT and fluorescence.2017

    • Author(s)
      Ohta M, Tokunaga M, Sakaguchi M, Hatoyama K, Gonda K.
    • Organizer
      A3 Foresight 9th Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛍光1粒子イメージングでがんの病態を可視化する2017

    • Author(s)
      権田幸祐
    • Organizer
      第106回日本病理学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Highly sensitive imaging of cancer with functional nanoparticles.2016

    • Author(s)
      Gonda K, Ohuchi N.
    • Organizer
      252nd American Chemical Society National Meeting
    • Place of Presentation
      Pennsylvania Convention Center, Philadelphia, PA, USA.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 権田研究室ホームページ

    • URL

      http://www.medphys.med.tohoku.ac.jp/gondalab/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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