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Spatio-temporal regulation and tissue basis of functional innate-adaptive immune cooperation in the lymph node

Research Project

Project/Area Number 16H05204
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionNiigata University

Principal Investigator

Katakai Tomoya  新潟大学, 医歯学系, 教授 (00324682)

Research Collaborator TAKEUCHI Arata  新潟大学, 医歯学系, 准教授 (00360579)
KANDA Yasuhiro  新潟大学, 医歯学系, 助教 (00436768)
OZAWA Madoka  新潟大学, 医歯学系, 特任助手 (50548740)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywordsリンパ節 / ストローマ細胞 / 組織区画 / 髄質 / 免疫細胞動態 / 自然ー獲得免疫連携 / ケモカイン / 自然免疫 / 獲得免疫 / 生体イメージング
Outline of Final Research Achievements

Lymph node is an important organ for immune responses. In this study, we focused on a tissue area, medulla-cortex transitional region (MCT), in which innate and adoptive immune cells are co-localized and unique stromal cells support the tissue. Three dimensional tissue structure, cell composition, molecular expression, and the motility/interaction of immune cells were investigated in detail. Consequently, we found previously less understood tissue subcompartments and unique stromal cell subsets, showing that at least 6 stromal cell subtypes are present in lymph node. These findings also suggest an innate-adoptive immune activation pathway through the medullary side. Moreover, transcription factor NFkB plays crucial role in the differentiation of multiple stromal cell types in the lymph node.

Academic Significance and Societal Importance of the Research Achievements

リンパ節の組織構造とストローマ細胞の関係がより明確になったことから、感染症やアレルギー性疾患、自己免疫疾患、癌などのさまざまな疾患においてこれらがどのように変化し、病態と関連するのかを理解する手がかりとなる。多様なストローマ細胞がリンパ節の各部分で異なる働きを持つことから、それぞれを人為的にコントロールする手段が見つかれば免疫応答を細かく制御することが可能となり、複雑な病気を治療するための基盤となる。リンパ節髄質における免疫応答誘導に関する理解が進めば、新規のワクチン開発などに向けた効果的な免疫惹起の手法が見つかる可能性がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (25 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (4 results)

  • [Int'l Joint Research] Cantonal Hospital St. Gallen(スイス)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Essential Role of Canonical NF-κB Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs2018

    • Author(s)
      Bogdanova Dana、Takeuchi Arata、Ozawa Madoka、Kanda Yasuhiro、Rahman M. Azizur、Ludewig Burkhard、Kinashi Tatsuo、Katakai Tomoya
    • Journal Title

      The Journal of Immunology

      Volume: 201 Issue: 12 Pages: 3580-3586

    • DOI

      10.4049/jimmunol.1800539

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A distinct subset of fibroblastic stromal cells constitutes the cortex-medulla boundary subcompartment of the lymph node2018

    • Author(s)
      Takeuchi A, Ozawa M, Kanda Y, Kozai M, Ohigashi I, Kurosawa Y, Rahman MA, Kawamura T, Shichida Y, Umemoto E, Miyasaka M, Ludewig B, Takahama Y, Nagasawa T, Katakai T.
    • Journal Title

      Frontiers in Immunology

      Volume: 9 Pages: 2196-2196

    • DOI

      10.3389/fimmu.2018.02196

    • NAID

      120006937683

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualizing the Rapid and Dynamic Elimination of Allogeneic T Cells in Secondary Lymphoid Organs2018

    • Author(s)
      Kanda Yasuhiro、Takeuchi Arata、Ozawa Madoka、Kurosawa Yoichi、Kawamura Toshihiko、Bogdanova Dana、Iioka Hidekazu、Kondo Eisaku、Kitazawa Yusuke、Ueta Hisashi、Matsuno Kenjiro、Kinashi Tatsuo、Katakai Tomoya
    • Journal Title

      The Journal of Immunology

      Volume: 201 Issue: 3 Pages: 1062-1072

    • DOI

      10.4049/jimmunol.1700219

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices.2018

    • Author(s)
      Katakai T.
    • Journal Title

      Methods Mol Biol.

      Volume: 1763 Pages: 29-42

    • DOI

      10.1007/978-1-4939-7762-8_4

    • ISBN
      9781493977611, 9781493977628
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リンパ節のストローマ構造とリンパ球動態2018

    • Author(s)
      片貝 智哉
    • Journal Title

      リンパ学

      Volume: 41 Pages: 20-24

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 蛍光イメージングで探るリンパ節の動的細密構造2018

    • Author(s)
      片貝 智哉
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 69 Pages: 326-332

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Journal Article] Extensively reorganized systemic lymph nodes provide a feasible environment for self-reactivity in lupus-prone NZB/NZW F1 mice.2017

    • Author(s)
      Kurosawa Y., Ozawa M., Kanda Y., Takeuchi A., Kawamura T., Narita I., Katakai T.
    • Journal Title

      Int Immunol.

      Volume: 29 Issue: 12 Pages: 567-579

    • DOI

      10.1093/intimm/dxx066

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Essential role of CCL21 in establishment of central self-tolerance in T cells.2017

    • Author(s)
      Kozai M, Kubo Y, Katakai T, Kondo H, Kiyonari H, Schaeuble K, Luther SA, Ishimaru N, Ohigashi I, Takahama Y.
    • Journal Title

      Journal of Experimental Medicine

      Volume: 214 Issue: 7 Pages: 1925-1935

    • DOI

      10.1084/jem.20161864

    • NAID

      120006312717

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microenvironmental control of high-speed interstitial T cell migration in the lymph node.2016

    • Author(s)
      Katakai T, Kinashi T.
    • Journal Title

      Front Immunol

      Volume: 7 Pages: 194-194

    • DOI

      10.3389/fimmu.2016.00194

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] リンパ節の組織微小環境に制御されるT細胞の高速遊走2016

    • Author(s)
      片貝智哉
    • Journal Title

      生化学

      Volume: 88 Pages: 615-620

    • NAID

      40020998185

    • Related Report
      2016 Annual Research Report
  • [Journal Article] リンパ節におけるTリンパ球の動態制御機構2016

    • Author(s)
      片貝智哉
    • Journal Title

      新潟医学会雑誌

      Volume: 130 Pages: 269-274

    • NAID

      120006759537

    • Related Report
      2016 Annual Research Report
  • [Presentation] Live imaging of the allogeneic T cell rejection in secondary lymphoid organs2018

    • Author(s)
      Kanda Y, Takeuchi A, Ozawa M, Kinashi T, Matsuno K, Katakai T
    • Organizer
      第47回日本免疫学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] DRC is a distinct subset of fibroblastic stroll cells construct the cortex-medulla boundary subcompartment and perform specific function in lymph node2018

    • Author(s)
      Takeuchi A, Ozawa M, Kanda Y, Katakai T
    • Organizer
      第47回日本免疫学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Conditional inactivation of canonical NF-kB activity in the fibroblastic stromal cells of secondary lymphoid organs2018

    • Author(s)
      Katakai T, Bogdanova D, Takeuchi A, Ozawa M, Kanda Y, Ludewig B, Kinashi T
    • Organizer
      第47回日本免疫学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fine structure of multilayered lymph node subcompartments and fibroblastic stromal cell subsets2018

    • Author(s)
      Tomoya Katakai
    • Organizer
      CIML/Japan joint symposium for immunology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 二次リンパ器官の発達とストローマ細胞文化におけるNFkBシグナルの意義2018

    • Author(s)
      片貝智哉
    • Organizer
      第5回 先進イメージング医学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リンパ節のストローマ構造とリンパ球動態2017

    • Author(s)
      片貝智哉
    • Organizer
      第41回 日本リンパ学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Six different types of mesenchymal stromal cells provide the framework of multi-layered sub compartments in lymph node.2017

    • Author(s)
      Takeuchi A., Ozawa M., Kanda Y., Ohigashi I., Kawamura T., Umemoto E., Miyasaka M., Ludewig B., Takahama Y., Nagasawa T., Katakai T.
    • Organizer
      第46回 日本免疫学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Live imaging of the allogeneic T cell elimination in secondary lymphoid organs.2017

    • Author(s)
      Kanda Y., Takeuchi A., Ozawa M., Matsuno K., Kinashi T., Katakai T.
    • Organizer
      第46回 日本免疫学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Visualizing the rapid and dynamic elimination of allogeneic T cells in secondary lymphoid organs2016

    • Author(s)
      Kanda Y, Takeuchi A, Ozawa M, Kawamura T, Matsuno K, Kinashi T, Katakai T
    • Organizer
      第45回日本免疫学会総会・学術集会
    • Place of Presentation
      沖縄国際会議場(沖縄県那覇市)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
  • [Remarks] 新潟大学大学院医歯学総合研究科 免疫・医動物学分野

    • URL

      https://www.med.niigata-u.ac.jp/zoo/welcome.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 新潟大学医学部医学科・大学院医歯学総合研究科(医学系)|News&Topics|研究成果

    • Related Report
      2018 Annual Research Report
  • [Remarks] 新潟大学大学院医歯学総合研究科/医学部医学科 免疫・医動物学分野

    • URL

      http://www.med.niigata-u.ac.jp/zoo/welcome.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 新潟大学医歯学総合研究科/医学部医学科 免疫・医動物学分野

    • URL

      http://www.med.niigata-u.ac.jp/zoo/welcome.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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