Analysis of human sarcoma stem cells and memory T stem cells
Project/Area Number |
16H05451
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
Keywords | 肉腫幹細胞 / 免疫記憶幹細胞 / 代謝 / 癌抗原 / 骨肉腫幹細胞 / がん抗原 / 骨肉腫 / 好気的解糖系 / 免疫学 / 病理学 / 癌 |
Outline of Final Research Achievements |
We identified sarcoma stem cell antigen LIN28B which conferred higher tumor-initiating ability and chemo-resistance to osteosarcoma stem cells. LIN28B suppressed OXPHOS and increased glycolysis dependency in osteosarcoma stem cells. Glycolysis inhibitors could suppress in vitro tumorigenesis of osteosarcoma stem cells. We also identified a novel human memory T stem cells, "Young Memory T cells". Young memory T cells showed the chemo-resistance, differentiation capacity and superior growth ability upon T cell receptor stimulation. T cells recognizing anti-viral antigen and anti-tumor-associated antigen could be induced from young memory T cells derived from sarcoma patients who received high-dose chemotherapy.
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Academic Significance and Societal Importance of the Research Achievements |
骨肉腫をはじめとする高い悪性形質を示す肉腫は時に若年者に発症し,化学療法不応例症例の予後は不良である.本研究成果により,解糖系阻害によって腫瘍増殖能が抑制される可能性,抗がん剤の効果が増強される可能性が示唆された. また免疫記憶幹細胞を制御できれば,がんワクチンやT細胞移入療法による免疫治療の効果をより高められる可能性がある.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Clonal analysis revealed functional heterogeneity in cancer stem-like cell phenotypes in uterine endometrioid adenocarcinoma.2019
Author(s)
Tabuchi Y, Hirohashi Y, Hashimoto S, Mariya T, Asano T, Ikeo K, Kuroda T, Mizuuchi M, Murai A, Uno S, Kawai N, Kubo T, Nakatsugawa M, Kanaseki T, Tsukahara T, Saito T, Torigoe T.
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Journal Title
Exp Mol Pathol
Volume: 106
Pages: 78-88
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long-term survival of a pancreatic cancer patient immunized with an SVN-2B peptide vaccine.2018
Author(s)
Shima H, Kutomi G, Satomi F, Imamura M, Kimura Y, Mizuguchi T, Watanabe K, Takahashi A, Murai A, Tsukahara T, Kanaseki T, Hirohashi Y, Iwayama Y, Tsuruma T, Kameshima H, Sato N, Torigoe T, Takemasa I.
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Journal Title
Cancer Immunol Immunother.
Volume: 67
Issue: 10
Pages: 1603-1609
DOI
Related Report
Peer Reviewed
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[Journal Article] Cancer-associated oxidoreductase ERO1-α promotes immune escape through up-regulation of PD-L1 in human breast cancer.2017
Author(s)
Tanaka T, Kutomi G, Kajiwara T, Kukita K, Kochin V, Kanaseki T, Tsukahara T, Hirohashi Y, Torigoe T, Okamoto Y, Hirata K, Sato N, Tamura Y.
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Journal Title
Oncotarget
Volume: 2016
Issue: 15
Pages: 24706-24718
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] The future of immunotherapy for sarcoma.2016
Author(s)
Tsukahara T , Emori M, Murata K, Mizushima E, Shibayama Y, Kubo T, Kanaseki T, Hirohashi Y, Yamashita T, Sato N, Torigoe T.
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Journal Title
Expert Opin Biol Ther
Volume: 16
Pages: 1049-1057
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Identification of a novel human memory T cell population with the characteristics of stem-like chemo-resistance2016
Author(s)
Murata K, Tsukahara T, Emori M, Shibayama Y, Mizushima E, Matsumiya H, Yamashita K, Kaya M, Hirohashi Y, Kanaseki T, Kubo T, Himi T, Ichimiya S, Yamashita T, Sato N, Torigoe T
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Cancer-associated oxidoreductase ERO1-α drives the production of VEGF via oxidative protein folding and regulating the mRNA level2016
Author(s)
Tanaka T, Kutomi G, Kajiwara T, Kukita K, Kochin V, Kanaseki T, Tsukahara T, Hirohashi Y, Torigoe T, Okamoto Y, Hirata K, Sato N, Tamura Y.
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Journal Title
Br J Cancer
Volume: 114
Pages: 1227-1234
NAID
Related Report
Peer Reviewed
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[Journal Article] Hypoxia augments MHC class I antigen presentation via facilitation of ERO1-α-mediated oxidative folding in murine tumor cells.2016
Author(s)
Kajiwara T, Tanaka T, Kukita K, Kutomi G, Saito K, Okuya K, Takaya A, Kochin V, Kanaseki T, Tsukahara T, Hirohashi Y, Torigoe T, Hirata K, Sato N, Tamura Y.
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Journal Title
Eur J Immunol.
Volume: 46
Pages: 2842-2851
Related Report
Peer Reviewed
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