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Search for aflatoxigenic and atoxigenic fungi in the fields to prevent aflatoxin contamination

Research Project

Project/Area Number 16H05785
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied microbiology
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KUSHIRO Masayo  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, ユニット長 (40353932)

Co-Investigator(Kenkyū-buntansha) 矢部 希見子  福井工業大学, 環境情報学部, 教授 (70158054)
Research Collaborator LOLADZE Alexander  国際トウモロコシ, 小麦改良センター
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsかび毒産生菌 / アフラトキシン / DV-AM法 / 菌類 / カビ毒 / ジクロルボス-アンモニア法 / 応用微生物 / 食品安全性
Outline of Final Research Achievements

Under the cooperation of domestic and foreign researchers, we demonstrated a direct isolation of Aspergillus flavus, a major aflatoxin-producing fungus and succeeded in acquisition of strains with different toxigenesis characteristic. The DV-AM method was shown to be effective for the acquisition of both aflatoxigenic and atoxigenic strains practically. In the same time, we modified the DV-AM method in sensitivity, and using this improved DV-AM method we obtained many aflatoxigenic isolates from soils samples of some cold areas and non-farming areas. As an inexpensive and highly sensitive visible technique, the DV-AM method was introduced in some invited lectures in foreign countries. By an article published in Mycoscience (2017), we won the 16th Hiratsuka Award in the Mycological Society of Japan.

Academic Significance and Societal Importance of the Research Achievements

DV-AM法は、アフラトキシン研究の第一人者である共同研究者の長年の研究成果に端を発する、アフラトキシン産生菌におけるアフラトキシン生合成機構の制御を基盤とした、高感度な毒素産生菌判別法であり、学術的に価値が高い。本研究により、感度がさらに高い改良DV-AM法が開発され、その適用範囲が広くなったことが実証された。改良DV-AM法は、高価な機器や煩雑な技術を必要としない、シンプルかつ安価な可視判別技術として、アフラトキシンによる農産物の被害が問題となっている諸外国で普及しつつあることから、社会的意義も大きい。農作物・食品以外の方面からも関心が寄せられ、DV-AM法のデモを行い教授した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] 国際トウモロコシ・小麦改良センター(CIMMYT)(メキシコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 保健・スポーツ省(Ministry of Health and Sports)(ミャンマー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 国際トウモロコシ・小麦改良センター(CIMMYT)(メキシコ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] 国際トウモロコシ・小麦改良センター(CIMMYT)(メキシコ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Occurrence of aflatoxins in processed chili pepper sold in Myanmar2019

    • Author(s)
      Aye Chaw Nu Nu、Nakagawa Hiroyuki、Kushiro Masayo
    • Journal Title

      JSM Mycotoxins

      Volume: 69 Issue: 1 Pages: 9-13

    • DOI

      10.2520/myco.69-1-4

    • NAID

      130007609196

    • ISSN
      0285-1466, 1881-0128
    • Year and Date
      2019-01-31
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Screening of aflatoxigenic fungi from Thai rice2018

    • Author(s)
      Aye Chaw Nu Nu、Sugiura Yoshitsugu、Kushiro Masayo
    • Journal Title

      Bulletin of the NARO Food Research

      Volume: 2 Pages: 1-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos-Ammonia (DV-AM) Method2018

    • Author(s)
      Kushiro Masayo、Hatabayashi Hidemi、Yabe Kimiko、Loladze Alexander
    • Journal Title

      Toxins

      Volume: 10 Issue: 7 Pages: 263-263

    • DOI

      10.3390/toxins10070263

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Improvement of the Culture Medium for the Dichlorvos-Ammonia (DV-AM) Method to Selectively Detect Aflatoxigenic Fungi in Soil2018

    • Author(s)
      Yabe Kimiko、Ozaki Haruna、Maruyama Takuya、Hayashi Keisuke、Matto Yuki、Ishizaka Marika、Makita Takeru、Noma Syun-ya、Fujiwara Kousuke、Kushiro Masayo
    • Journal Title

      Toxins

      Volume: 10 Issue: 12 Pages: 519-519

    • DOI

      10.3390/toxins10120519

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation of minor aflatoxigenic fungi using dichlorvos-ammonia (DV-AM) method2018

    • Author(s)
      Kushiro Masayo、Hatabayashi Hidemi、Nakagawa Hiroyuki、Yabe Kimiko
    • Journal Title

      JSM Mycotoxins

      Volume: 68 Issue: 1 Pages: 13-18

    • DOI

      10.2520/myco.68-1-4

    • NAID

      130006405060

    • ISSN
      0285-1466, 1881-0128
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 培養による毒素産生型アスペルギルス属かびの検出法-ジクロルボス-アンモニア蒸気法(DV-AM法)2018

    • Author(s)
      久城真代、矢部希見子
    • Journal Title

      ニューフードインダストリー

      Volume: 60(3) Pages: 21-27

    • NAID

      40021488791

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Analysis of the Masked Metabolite of Deoxynivalenol and Fusarium Resistance in CIMMYT Wheat Germplasm2017

    • Author(s)
      Nakagawa Hiroyuki、He Xinyao、Matsuo Yosuke、Singh Pawan、Kushiro Masayo
    • Journal Title

      Toxins

      Volume: 9 Issue: 8 Pages: 238-238

    • DOI

      10.3390/toxins9080238

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Application of newly-developed dichlorvos-ammonia (DV-AM) method to direct isolation of aflatoxigenic fungi from field soils2017

    • Author(s)
      Masayo Kushiro, Hidemi Hatabayashi, Yazhi Zheng, Kimiko Yabe
    • Journal Title

      Mycoscience

      Volume: 58 Issue: 2 Pages: 85-94

    • DOI

      10.1016/j.myc.2016.10.002

    • NAID

      40021141357

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improvement of mobile phase in thin-layer chromatography for aflatoxins and analysis of the effect of dichlorvos in aflatoxigenic fungi2017

    • Author(s)
      Masayo Kushiro, Hidemi Hatabayashi, Hiroyuki Nakagawa, Kimiko Yabe
    • Journal Title

      JSM Mycotoxins

      Volume: 67 Issue: 1 Pages: 5-6

    • DOI

      10.2520/myco.67-1-5

    • NAID

      130005404227

    • ISSN
      0285-1466, 1881-0128
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ジクロルボス-アンモニア(DV-AM)法を用いた養蚕場におけるアフラトキシン生産菌の検出2019

    • Author(s)
      西脇瑛舜, 湯下実穂, 山田悠貴, 矢部希見子
    • Organizer
      日本マイコトキシン学会第83回学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ジクロルボス-アンモニア(DV-AM)法を用いた複数地域におけるアフラトキシン生産菌のスクリーニング2019

    • Author(s)
      山本侑加, 角屋直暉, 舘大樹, 矢部希見子
    • Organizer
      日本マイコトキシン学会第83回学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アフラトキシン生産菌検出法「ジクロルボス-アンモニア(DV-AM)法」の培地条件の改良2018

    • Author(s)
      矢部希見子, 尾嵜春菜, 丸山拓也, 林敬祐, 松任佑機, 石坂麻里香, 牧田健, 野間竣陽, 藤原幸祐, 久城真代
    • Organizer
      日本マイコトキシン学会第82回学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The dichlorvos-ammonia method for visible detection of aflatoxigenic fungi from environments2018

    • Author(s)
      Kimiko Yabe, Masayo Kushiro
    • Organizer
      3rd World Biotechnology Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Isolation of minor aflatoxigenic fungi using dichlorvos-ammonia (DV-AM) metho2018

    • Author(s)
      Chaw Nu Nu Aye, Yoshitsugu Sugiura, Masayo Kushiro
    • Organizer
      日本マイコトキシン学会第81回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ジクロルボス-アンモニア(DV-AM)法によるマイナーなアフラトキシン産生菌の同定2018

    • Author(s)
      久城真代、畑林秀美、中川博之、矢部希見子
    • Organizer
      日本マイコトキシン学会第81回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 培養による毒素産生型アスペルギルス属菌判別法の高度化2017

    • Author(s)
      久城真代
    • Organizer
      公益財団法人飯島藤十郎記念食品科学振興財団第29回学術講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] アフラトキシン生産菌の高感度可視検出法「ジクロルボス-アンモニア(DV-AM)法」の開発2016

    • Author(s)
      矢部希見子
    • Organizer
      日本マイコトキシン学会第79回学術講演会
    • Place of Presentation
      文部科学省研究交流センター(茨城県つくば市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高感度可視検出法「DV-AM法」による圃場土壌からのアフラトキシン生産菌の分離2016

    • Author(s)
      久城真代
    • Organizer
      日本マイコトキシン学会第79回学術講演会
    • Place of Presentation
      文部科学省研究交流センター(茨城県つくば市)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 食品研究部門 食品安全研究領域 食品化学ハザードユニット

    • URL

      http://www.naro.affrc.go.jp/nfri-neo/introduction/chart/0402/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 福井工業大学 情報科学科 環境・食品科学科

    • URL

      http://www.fukui-ut.ac.jp/ut/subject/environment/

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2022-08-05  

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